Effects of Elevated CO_2 on Consumption and Biochemical Substances of Flea Beetle,Agasicles hygrophila
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摘要: 在实验室条件下,分别测定了高CO2浓度(750μL·L-1)和对照CO2浓度(370μL·L-1)2个不同CO2浓度下莲草直胸跳甲幼虫取食量的变化,比较3龄幼虫体内营养物质含量和消化酶活性等指标的变化。结果表明,高CO2浓度处理下,莲草直胸跳甲幼虫取食量显著增加;3龄幼虫体内蛋白含量较对照CO2浓度处理幼虫的蛋白显著增加,总氨基酸含量差异不显著;莲草直胸跳甲3龄幼虫体内消化酶(淀粉酶和胃蛋白酶)的酶活力显著降低。高CO2浓度影响莲草直胸跳甲幼虫的取食量、营养物质含量和消化酶活性,对其生长发育有一定的影响。Abstract: The consumption of flea beetle,A.hygrophila,was examined in different CO2concentration(750μL·L-1and 370μL·L-1)in closed-dynamic CO2 chamber.And the nutrient compositions and digestive enzymes activities of flea beetle were both measured and analyzed.The results showed that significantly higher consumption of A.hygrophilalarva was observed in elevated CO2 relative to 370μL·L-1.The protein increased in the 3th star larva of A.hygrophilain750μL·L-1 compared with 370μL·L-1 significantly,and there was no significant difference in total amino acid between the two treatments.However,the activities of amylase and pepsin activities were both declined in elevated CO2 compared with370μL·L-1.Elevated CO2 can affect the consumption,nutrient compositions and digestive enzymes activities of A.hygrophilalarva,and also on the growth and development of A.hygrophila.
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Key words:
- Agasicles hygrophila /
- elevated CO2 concentration /
- consumption /
- nutrient /
- digestive enzymes
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