摘要:
为了解2012-2014年福建省流行PEDV毒株E和M基因的变异情况,对19株PEDV的E基因和18株PEDV的M基因进行克隆和序列分析。结果表明:与CV777标准毒株相比较,福建流行PEDV毒株的E和M基因存在编码的部分氨基酸变异。19株PEDV E基因的核苷酸之间的同源性为97.4%~100.0%,与attenuated DR13弱毒株和CV777标准株同源性分别为97.4%~100.0%和97.0%~99.6%。18株PEDV M基因的核苷酸之间的同源性为97.4%~100.0%,与attenuated DR13弱毒株和CV777标准株同源性分别为97.2%~99.6%和97.5%~98.4%。核苷酸遗传进化树表明福建流行PEDV毒株的E基因与CV777亲缘关系较近,而M基因与2008年泰国流行的KU05CB08毒株亲缘关系较近,是个新的基因型,可能源于泰国。
Abstract:
To investigate the proteinvariationsin the porcine epidemic diarrhea virus(PEDV),19 Eand 18 M genes from the viruses collected from different farms in Fujian in 2012-2014 were cloned and sequenced.The results indicated that the PEDVEand Mgenes were conservative.There were individual variants foundonlyonamino acids,as compared with CV777 strain.On the nucleotide sequences,the E geneswere 97.4%-100.0% homogenous among themselves,97.4%-100.0% homogenous with attenuated DR13 strain,and 97.0%-99.6% homogenous with CV777 strain.For the M genes,they were 97.4%-100.0% homogenous among the 18 of them,97.2%-99.6% homogenous with attenuated DR13,and 97.5%-98.4% homogenous with CV777.A phylogenetic analysis indicated that the Egenes of the 19 PEDVs were closely related to CV777,and the Mgenes of the 18 PEDVs closely related to KU05CB08.The M gene wasa new variant,which might bear an origin of strains from Thailand.
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