小菜蛾气味结合蛋白PxylOBP13基因克隆与序列分析

覃江梅; 程小娟; 魏辉; 蔡立君; 尤民生

doi:10.19303/j.issn.1008-0384.2016.04.009

小菜蛾气味结合蛋白PxylOBP13基因克隆与序列分析

doi: 10.19303/j.issn.1008-0384.2016.04.009

覃江梅^1,2,3,4,
程小娟^1,2,3,5,
魏辉⁶,
蔡立君^1,2,3,4, ,,
尤民生^1,2,3,4, ,

1.
福建农林大学应用生态研究所, 福建福州 350002;
2.
闽台特色作物病虫生态防控协同创新中心, 福建福州 350002;
3.
农业部闽台作物有害生物综合治理重点实验室, 福建福州 350002;
4.
福建农林大学植物保护学院, 福建福州 350002;
5.
福建农林大学生命科学学院, 福建福州 350002;
6.
福建省农业科学院植物保护研究所, 福建福州 350013

基金项目:

国家自然科学基金项目(31301666、31230061、31320103922);福建省自然科学基金(2014J05031)

详细信息

作者简介:
覃江梅(1990-),女,硕士生,研究方向:农业昆虫与害虫防治(E-mail:qinjiangmei0325@163.com)

通讯作者:
尤民生(1954-),男,教授,研究方向:农业昆虫防治与综合治理(E-mail:msyou@iae.fjau.edu.cn);蔡立君(1982-),女,博士,研究方向:农业昆虫防治与综合治理(E-mail:cai-lijun@live.cn)

尤民生(1954-),男,教授,研究方向:农业昆虫防治与综合治理(E-mail:msyou@iae.fjau.edu.cn);蔡立君(1982-),女,博士,研究方向:农业昆虫防治与综合治理(E-mail:cai-lijun@live.cn)

中图分类号: Q96
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出版历程
- 收稿日期: 2016-01-23
- 修回日期: 2016-02-25
- 刊出日期: 2016-04-28

Cloning and Sequence Analysis of an Odorant Binding Protein Gene PxylOBP13 in the Diamondback Moth, Plutella xylostella (Lepidoptera:Plutellidae)

QIN Jiang-mei^1,2,3,4,
CHENG Xiao-juan^1,2,3,5,
WEI Hui⁶,
CAI Li-jun^{1,2,3,4
, ,},
YOU Min-sheng^{1,2,3,4
, ,}

1.
Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
3.
Key Laboratory of Integrated Pest Management of Fujian and Taiwan, China Ministry of Agriculture, Fuzhou, Fujian 350002, China;
4.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
5.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
6.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 小菜蛾是为害十字花科蔬菜的重要害虫, 干扰其嗅觉识别功能是实现小菜蛾有效治理的重要途径。本研究克隆并鉴定了1个小菜蛾气味结合蛋白基因, 命名为PxylOBP13(GenBank登录号为KT156679);该基因开放阅读框全长387bp, 编码128个氨基酸;预测蛋白分子量为14.33KDa, 等电点为7.55, N端无信号肽序列, 有3个疏水性区域, 具有气味结合蛋白家族的保守结构域和6个保守的半胱氨酸残基, 与6种鳞翅目昆虫的气味结合蛋白氨基酸序列一致性达50%以上。研究结果可为进一步研究小菜蛾气味结合蛋白功能, 阐明小菜蛾嗅觉机制, 制定科学有效的治理措施提供依据。
- 小菜蛾 /
- 气味结合蛋白 /
- 克隆 /
- 序列分析
Abstract: The diamondback moth (DBM), Plutella xylostella (L.) is a key insect pest on cruciferous plants all over the world. Olfactory perception is critical for survival and reproduction in insects, and correspondingly provides a new target to control DBM. Hereby, an odorant binding protein (OBP) gene from P.xylostella,PxylOBP13 (GenBank accession number:KT156679) was cloned; the bio-information characteristics were analyzed; and a phylogenetic tree was constructed. The results showed that the full-length of open reading frame of PxylOBP13 is 387 bp and encodes 128 amino acids; PxylOBP13 contains 6 conserved cysteine residues and the conserved domain of odorant binding protein family, and 3 hydrophobic regions which are potential binding sites for hydrophobic odorants. The protein molecular mass was predicted as 14.33 kDa with an isoelectric point of 7.55, but the N-terminal without signal peptide; the similarity of sequence between PxylOBP13 and six lepidopteran insects can reach above 50%. The results can provide theoretical support for further research on the function of OBPs in DBM and development of effective management of DBM based on olfactory mechanism.
- Plutella xylostella /
- odorant binding protein /
- cloning /
- sequence analysis

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参考文献(8)

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