Effects of Probiotics Fermentation Products on Meat Production and Serum Indices of Mindong Goats
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摘要: 为研究益生菌(酵母菌和乳酸菌)发酵产物对闽东山羊生产性能和血液生化指标的影响,选取64头年龄、性别和体况相近的闽东山羊,随机分成试验组和对照组(分圈饲养),每组32头,公母各半,饲喂1个月,测定64头闽东山羊试验后体重变化;试验组和对照组各随机抽取16头采血,测定各项血清生化指标。结果表明,试验组的期末重和平均日增重显著(P < 0.05)高于对照组;试验组的白蛋白含量、白球比和碱性磷酸酶含量极显著(P < 0.01)降低,谷丙转氨酶含量、总胆固醇含量、高密度脂蛋白含量和低密度脂蛋白含量显著(P < 0.05)降低,球蛋白含量和IgA含量极显著(P < 0.01)升高;酶联法测定IgE和IgM的含量极显著(P < 0.01)升高,IgA显著(P < 0.05)升高。说明酵母菌和乳酸菌发酵提取混合物能够提高闽东山羊的生产性能、机体蛋白的代谢效率和免疫力。Abstract: Effects of including fermentation products of yeasts and lactic acid bacteria(LAB) in feed on the meat production and serum indices of Mindong goats were studied.Thirty-two male and 32 female goats of similar age and physical conditions were randomly and evenly divided into a treatment and a control group for a month-long feeding test.At the end, blood samples from 16 randomly picked goats in each group were collected for serum analysis.The results showed that the averaged final body weight and daily weight gain of the goats in the treatment group were significantly higher than those of control(P < 0.05).In the treatment goats, the serum albumin, globulin ratio, and alkaline phosphatase were extremely significantly lower(P < 0.01), glutamic-pyruvic transaminase, total cholesterol, high-density lipoprotein, and low-density lipoprotein significantly lower(P < 0.05), while the immune globulin and IgA extremely significantly higher(P < 0.01) than control. As tested by the enzyme-linked method, the immune globulin IgE and IgM in the treatment goats significantly increased at P < 0.01 level, while IgA significantly increased at P < 0.05 over control. It indicated that the dietetic inclusion of mixture of the yeast and LAB fermentation extracts could improve the meat production, metabolic efficiency and immunity of Mindong goats.
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Key words:
- probiotics /
- yeast /
- lactic acid /
- fermentation products /
- meat production /
- serum indices /
- Mindong goat
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表 1 基础日粮组成和营养水平(风干基础)
Table 1. Ingredients and nutritional compositions of experimental diets (on dry basis)
日粮组成 含量 营养水平 含量 干草/ % 65 消化能/(MJ·kg-1DW) 10.8 玉米/ % 15 粗脂肪/ % 2.70 豆粕/ % 8 中性洗涤纤维/ % 58.00 豆渣/ % 5 酸性洗涤纤维/ % 32.00 麸皮/ % 4.5 粗蛋白/ % 8.80 氯化钠/ % 0.5 磷酸二氢钙/ % 1 预混料/ % 1 表 2 不同处理对闽东山羊体重的影响
Table 2. Effect of treatment diet on body weight of Mindong goats
项目 对照组 试验组 初重/ kg 6.91±1.55 7.08±1.45 末重/ kg 8.59±1.48b 9.84±1.46a 平均日增重/(g·d-1) 56.00±11.51b 92.13±11.83a 注:同一行数据后不同大写字母表示差异极显著(P < 0.01),不同小写字母表示差异显著(P < 0.05)。下表同。 表 3 不同处理对闽东山羊血清生化指标的影响
Table 3. Effects of treatment diet on serum biochemical indicators of Mindong goats
项目 对照组 试验组 总蛋白/(g·L-1) 70.57±2.13 69.65±5.96 白蛋白/(g·L-1) 28.42±2.33A 21.7±3.78B 球蛋白/(g·L-1) 42.15±3.28B 47.97±3.47A 白蛋白/球蛋白 0.68±0.09A 0.45±0.07B IgM/(g·L-1) 0.03±0.01 0.03±0.01 IgG/(g·L-1) 0.02±0.01 0.02±0.00 IgA/(g·L-1) 0.03±0.04b 0.10±0.00a 谷氨酰转肽酶/(U·L-1) 34.28±11.69 37.63±5.19 碱性磷酸酶/(U·L-1) 220.53±100.20A 74.73±19.15B 谷丙转氨酶/(U·L-1) 46.32±5.21a 37.17±7.09b 谷草转氨酶/(U·L-1) 101.52±20.37 109.35±13.89 谷草转氨酶/谷丙转氨酶 2.24±0.62 3.04±0.75 肌酸激酶/(U·L-1) 399.00±161.12 443.17±119.86 乳酸脱氢酶/(U·L-1) 302.45±52.03 263.65±10.82 总胆固醇/(mmol·L-1) 4.34±2.66a 2.12±0.67b 甘油三酯/(mmol·L-1) 0.21±0.12 0.18±0.07 高密度脂蛋白/(mmol·L-1) 1.78±0.49a 1.25±0.25b 低密度脂蛋白/(mmol·L-1) 2.47±2.22a 0.79±0.44b 表 4 不同处理对闽东山羊免疫球蛋白的影响
Table 4. Effect of treatment diet on immunoglobulin in Mindong goats
项目 对照组 试验组 IgE/(μg·L-1) 86.34±2.30B 107.49±2.16A IgA/(μg·L-1) 113.59±2.38b 121.99±3.09a IgM/(μg·L-1) 13.75±0.40B 20.18±0.39A IgG/(μg·L-1) 1253.20±32.89 1288.62±36.52 -
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