Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings
-
摘要: 自胰腺泛黄的雏半番鸭中分离获得1株病毒（命名为FJ1605株），经RT-PCR检测为鸭1型甲肝病毒，通过鸭胚中和试验发现该株病毒可被鸭1型甲肝病毒亚型（DHAV-1a）高免血清特异性中和，确定该株病毒为鸭1型甲肝病毒亚型。对该株病毒VP1基因进行分子特征分析，发现其核苷酸大小为714 bp，与GenBank登录的DHAV-1a同源性为98.1%~99.7%，与DHAV-1 FJ1220毒株同源性最高达99.7%；而与鸭2型甲肝病毒（DHAV-2）、鸭3型甲肝病毒（DHAV-3）VP1基因的核苷酸同源性仅分别为65.7%和68.3%左右。基于VP1基因的遗传进化分析表明，FJ1605分离株属鸭1型甲肝病毒亚型谱系。以该株病毒对7日龄雏半番鸭进行人工感染试验，可完全复制出同于临床病例的病变。以上结果显示，雏半番鸭也可感染DHAV-1a，且表现为胰腺泛黄。Abstract: A strain of duck hepatitis A virus 1 subtype a (DHAV-1a), coded as FJ1605, was isolated from the mule ducklings with pancreatitis. It was identified by RT-PCR analysis and a neutralization test in duck embryos that showed the strain specifically being neutralized by the anti-DHAV-1a hyperimmune serum. Molecular characteristics of VP1 gene of the virus was analyzed to indicate the nucleotide to be 714 bp in length with a homology with those of other DHAV-1a isolates ranging from 98.1% to 99.7% and sharing the greatest similarity to strain FJ1220.On the other hand, the homology between FJ1605 and DHAV-2 was merely 65.7%; and, that between FJ1605 and DHAV-3, approximately 68.3%. Phylogenetic analysis on the VP1 gene also showed FJ1605 to be in the viral lineage of DHAV-1a. Furthermore, an animal infection test clearly displayed the same clinical symptoms as those shown inclinical cases. Thus, it was concluded that the pancreatitis in the mule ducklings was caused by DHAV-1a.
-
Key words:
- DHAV-1a /
- mule duckling /
- VP1 gene
-
图 2 FJ1605 VP1基因核苷酸序列与其他鸭肝炎病毒的同源性
Figure 2. Homology of VP1 gene nucleotides of FJ1605 and other duck hepatitis virus strains
图 3 FJ1605毒株VP1基因遗传进化分析
Figure 3. Phylogenetic analysis on VP1 gene isolated from FJ1605
图 4 DHAV-1a VP1蛋白的亲水性、抗原指数及表面展示概率分析
Figure 4. Analyses on hydrophilicity, antigenic index and surface display probability of VP1 protein
表 1 参考毒株相关信息
Table 1. Information on reference viruses
登录号 名称 基因型 分离地 分离时间 KF924552 MPZJ1206 DHAV-1a 中国 2012 KC904272 FJ1220 DHAV-1a 中国 2012 KX507161 JX1405 DHAV-1a 中国 2014 KX507163 FJ1513 DHAV-1a 中国 2015 JQ804521 Du/CH/LGD/111238 DHAV-1a 中国 2011 DQ249299 03D DHAV-1 中国 2005* JX390984 FZ99 DHAV-1 中国 1999 GU9446771 MY DHAV-1 中国 2006 JQ316452 X DHAV-1 中国 2011* DQ864514 C80 DHAV-1 中国 2006* JX390983 FZ05 DHAV-1 中国 2005 DQ226541 R85952 DHAV-1 美国 2005* DQ219396 DRL62 DHAV-1 韩国 2005* JX390982 FZ86 DHAV-1 中国 1986 EF427899 CL DHAV-1 中国 2007* EF067924 90D DHAV-2 中国 2007* JF828996 Du/CH/LJS/090905 DHAV-3 中国 2009 EU755009 G DHAV-3 中国 1999 DQ812093 AP-04114 DHAV-3 韩国 2003 注：*表示基因序列提交时间。 
下载: 导出CSV
-
[1] OWENS R A, DI S, LI S F, et al. Virus Taxonomy:Ninth Report of the International Committee on Taxonomy of Viruses[C]//Ninth Report of the International Committee on Taxonomy of Viruses, 2011:1221-1234. [2] SAIF Y M. 禽病学[M]. 苏敬良, 高福, 索勋, 译. 11版. 北京: 中国农业出版社, 2005: 376-389. [3] 傅光华, 陈红梅, 黄瑜, 等.雏番鸭胰腺型鸭1型甲肝病毒分离鉴定及VP1基因分析[J].福建农业学报, 2012, 27(9):43-45. http://www.fjnyxb.cn/CN/abstract/abstract1994.shtml [4] 傅光华, 黄瑜, 傅秋玲, 等.致胰腺泛黄鸭1型甲肝病毒全基因组分子特征[J].微生物学报, 2014, 54(9):1082-1089. http://www.cnki.com.cn/Article/CJFDTOTAL-WSXB201409014.htm [5] 陈珍, 傅秋玲, 陈红梅, 等.胰腺型、经典型1型甲肝病毒对雏鸭的致病性差异[J].福建农业学报, 2013, 28(10):939-942. doi: 10.3969/j.issn.1008-0384.2013.10.002 [6] 傅秋玲, 陈珍, 黄瑜, 等.鸭1型甲肝病毒亚型的鉴定[J].中国兽医杂志, 2015, 51(7):36-38. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZGXJ201611001103.htm [7] 殷震, 刘景华.动物病毒学:第2版[M].北京:科学出版社, 1997:343-354. [8] 傅秋玲, 刘伟, 黄瑜, 等.鸭1型甲肝病毒亚型VP1蛋白单克隆抗体的研制及鉴定[J].中国畜牧兽医, 2017, 44(2):554-560. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZGXJ201611001103.htm [9] 郭玉璞, 蒋金书.鸭病[M].北京:北京农业大学出版社, 1988:30-31. [10] 陈红梅, 施少华, 程龙飞, 等.鹅源鸭1型甲肝病毒的分离与鉴定[J].福建农业学报, 2012, 27(11):1165-1168. doi: 10.3969/j.issn.1008-0384.2012.11.004 [11] DING C, ZHANG D. Molecular analysis of duck hepatitis virus 1[J]. Virology, 2007, 361:9-17. doi: 10.1016/j.virol.2007.01.007 [12] KIM M C, KWON Y K, JOH S J, et al. Molecular analysis of duck hepatitis virus type 1 reveal s a novel lineage close to t he genus parechovirus in t he family Picornaviridae[J]. J Gen Virol, 2006, 87:3307-3316. doi: 10.1099/vir.0.81804-0 [13] TSENG C H, KNOWLES N J, TSAI H J. Molecular analysis of duck hepatitis virus type 1 indicates t hat it should be assigned to a new genus[J]. Virus Res, 2007, 123:190-203. doi: 10.1016/j.virusres.2006.09.007 [14] KIM M C, KWON Y K, JOH S J, et al. Recent Koren isolates of duck hepatitis virus revealed t he presence of a new geno2 and serotype when compared to duck hepatitis virus type 1 type strain[J]. Arch Virol, 2007, 152:2059-2072. doi: 10.1007/s00705-007-1023-0 [15] FU Y, PAN M, WANG X Y, et al. Molecular detection and typing of duck hepatitis A virus directly from clinical specimens[J]. Vet Microbiol, 2008, 131(3/4):247-257. http://europepmc.org/abstract/med/18462894 -
点击查看大图
图(4) / 表(1)
计量
- 文章访问数: 1301
- HTML全文浏览量: 169
- PDF下载量: 283
- 被引次数: 0
