Molecular Epidemiology of Enzootic Nasal Tumor Virus on Goats in Fujian
-
摘要: 为明确2014-2017年福建省多地区羊发生的一种以流稀薄鼻液为主要症状疾病的病原，对该病进行流行病学调查、肿瘤组织切片观察，特异性PCR诊断以及核酸序列分析，结果显示该病在流行病学、临床症状、病理变化、组织病理变化上与国内外已报道的病例表现基本一致，RT-PCR鉴定结果为ENTV-2，其中7个分离株与ENTV-2CHN2（分离于中国）处于同一进化树分支，1个分离株与ENTV-SC（分离于中国）、ENTV-Shaanxi（分离于中国）处于同一进化树分支。说明近几年福建省流行的羊肿瘤病的病原为ENTV-2，为福建省加强该病的防治奠定基础。Abstract: The pathogen that caused a disease characterized by thin nasal discharge on goats in Fujian during 2014-2017 was investigated.An epidemiological survey of the disease was conducted with tumor tissue biopsy, specific PCR diagnosis, and nucleic acid sequence analysis. It was found that the disease had basically the sam eepidemiology, clinical symptoms, pathology, and histopathology as the similar cases reported domestically and abroad. RT-PCR identified the pathogen to be ENTV-2. Among the various isolated viruses, 7 were in the same phylogenetic tree branch as ENTV-2 CHN2, and one in the branch as ENTV-SC and ENTV-Shaanxi, which were all isolated in China.The results confirmed ENTV-2 to be the culprit of the disease in goats found in Fujian Province. Hopefully, with the information, a prevention measure and/or cure for the disease could be developed in the near future.
-
Key words:
- goat /
- enzootic nasal tumor /
- molecular epidemiology
-
图 2 羊头部病理解剖
注：鼻甲骨出血，筛骨萎缩。鼻腔中有腺瘤，呈菜花状、结节状或类圆柱状，周围无包膜，外观呈粉红色、表面不平、质地软脆，额窦内有积液或胶冻样渗出物。
Figure 2. Pathological anatomy of goat head
图 3 羊鼻内肿瘤部分病例PCR结果
注：M为DNA Marker 2000；NC为空白对照；1~5、6~16为部分病例。
Figure 3. PCR detection onenzootic nasal tumor virus of goats in clinic samples
-
[1] VITELLOZZI G, MUGHETTI L, PALMARINI M, et al. Enzootic intranasal tumour of goats in Italy[J]. journal of veterinary medicine series b-infectious diseases and veterinary public health, 1993, 40(7):459-468. http://europepmc.org/abstract/MED/8284960 [2] COUSENS C, MINGUIJON E, DALZIEL R G, et al. Complete sequence of enzootic nasal tumor virus, a retrovirus associated with transmissible intranasal tumors of sheep[J].Journal of virology, 1999, 73(5):3986-3993. http://europepmc.org/articles/PMC104177 [3] ORT N AURORA, COUSENS CHRISTINA, MINGUIJ N ESMERALDA, et al. Characterization of enzootic nasal tumour virus of goats:complete sequence and tissue distribution[J]. Journal of General Virology, 2003, 84(8):2245-2252. doi: 10.1099/vir.0.19125-0 [4] DELAS HERAS M, ORT N A, COUSENS C, et al. Enzootic nasal adenocarcinoma of sheep and goats[J]. Current Topics in Microbiology and Immunology, 2003, 275:201-203. http://www.ncbi.nlm.nih.gov/pubmed/12596900 [5] KAWASAKO K, OKAMOTO M, KUROSAWA T, et al. Enzootic intranasal tumour virus infection in apparently healthy sheep in Japan[J].The Veterinary Record, 2005, 157(4):118-120. doi: 10.1136/vr.157.4.118 [6] SCOCCO P, MARIOTTI F, CECCARELLI P, et al. Origin of enzootic intranasal tumor in the goat (Capra hircus):a glycohistochemical approach[J]. Veterinary Pathology, 2001, 38(1):98-104. doi: 10.1354/vp.38-1-98 [7] PERL S, YAKOBSON B, ORGAD-KLOPFER U, et al. Enzootic nasal tumour of sheep[J]. Veterinary Quarterly, 1987, 9(2):118-122. doi: 10.1080/01652176.1987.9694088 [8] SCOTT R, WALSH, NICOLLE M. et al. Full-length genome sequence analysis of enzootic nasal tumor virus reveals an unusually high degree of genetic stability[J].Virus Research, 2010, 151:74-87. doi: 10.1016/j.virusres.2010.04.002 [9] 林曦, 郝先谱, 赵振华, 等.山羊鼻内腺瘤和腺癌的病理学研究[J].畜牧兽医学报, 1995, 26(5):456-461. http://www.cnki.com.cn/Article/CJFDTOTAL-XMSY505.013.htm [10] 雷红宇, 苏建明, 宁玲忠, 等.一起山羊地方性鼻内肿瘤的调查与诊断[J].动物医学进展, 2006, 27(2):112-114. http://www.cnki.com.cn/Article/CJFDTOTAL-DYJZ201702030.htm [11] 刘菲, 冯迎春, 颜其贵, 等.四川一起山羊地方性鼻内肿瘤疫情的诊断[J].畜牧与兽医, 2011, 43(6):83-86. http://www.cnki.com.cn/Article/CJFDTOTAL-XMYS201106026.htm [12] 余远迪, 韦雷飞, 黄秀君, 等. 4例山羊鼻内肿瘤的诊断[J].动物医学进展, 2014, 35(4):129-131. http://www.cnki.com.cn/Article/CJFDTOTAL-DYJZ201404031.htm [13] PALMARINI M, MURA M, SPENCER T. Endogenous betaretroviruses of sheep:teaching new lessons in retroviral interference and adaptation[J]. Gen Virol, 2004, 85(1):1-13. doi: 10.1099/vir.0.19547-0 [14] SPENCER T, MURA M, GRAY C, et al. Receptor usage and fetal expression of ovine endogenous betaretroviruses:implications for coevolution of endogenous and exogenous retroviruses[J]. Virol, 2003, 77(1):749-753. doi: 10.1128/JVI.77.1.749-753.2003 [15] LIU SHAN-LU, MILLER A DUSTY. Transformation of madin-darby canine kidney epithelial cells by sheep retrovirus envelope proteins[J]. Journal of Virology, 2004, 79(2):927-933. http://www.ncbi.nlm.nih.gov/pubmed/15613321 -
下载:
点击查看大图
图(6)
计量
- 文章访问数: 1395
- HTML全文浏览量: 203
- PDF下载量: 136
- 被引次数: 0
