Aromatic Volatiles in Lentinula edodes Determined by Gas Chromatography-Ion Mobility Spectrometry
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摘要: 利用气相色谱-离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)技术分析不同香菇干样挥发性组分的指纹图谱。根据离子迁移数据利用热图聚类和主成分分析不同样品间的差异。热图聚类分析可将27份香菇材料分为2大类;PCA分析表明,前2个主坐标的方差贡献率分别为30.2%和26.9%。结果表明气相色谱偶联离子迁移谱技术对构建香菇挥发性物质指纹图谱及挥发性组分数据库具有潜在的应用价值。Abstract: Gas chromatography-ion mobility spectrometry (GC-IMS) was applied to analyze the aromatic components in 27 Lentinula edodes. The measurement data were subsequently subjected to the principal component, clustering and heatmap analyses. Accordingly, the mushrooms were clustered into two groups. Two principal components of the volatiles contributed 30.2% and 26.9% of the total variance among the mushroom samples. The analytical method and the established fingerprints on the chemical components would be useful for future studies on L. edodes.
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Key words:
- Lentinula edodes /
- volatile components /
- ion mobility spectrometry /
- gas chromatography
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表 1 挥发性组分的定性
Table 1. Volatiles identified by GC-IMS
物质名称 CAS号 保留时间
/s保留指数 漂移时间
/ms环己酮 C108941 223.162 885.3 1.1543 顺式-3-己烯-1-醇 C928961 210.023 866.8 1.6604 2-甲基丁酸-甲基丁酯 C868575 142.379 753.5 1.5251 乙酸乙酯 C141786 84.148 583.6 1.3291 甲基丙醛 C78842 81.549 571.4 1.2826 -
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