Process and Antioxidant Activity of Flavonoid Extract from Abelmoschus esculentus
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摘要: 采用微波辅助提取法提取黄秋葵中的黄酮类化合物，通过单因素和正交试验确定最佳条件：体积分数70%乙醇为提取溶剂，温度70℃，料液比1:40（g·mL-1），时间40 min，黄酮提取率2.78%，相对标准偏差RSD为0.635%，该方法提取率较高、稳定可行；采用DPPH和ABTS自由基法测定提取的黄酮类化合物抗氧化活性，IC50分别为1.21、1.06 mg·mL-1，表现为较强的抗氧化活性。Abstract: A microwave-assisted processes to extract flavonoids from Abelmoschus esculentus was optimized by a single factor and orthogonal experiment. By using 70% ethanol as the solvent, A. esculentus in a solid-liquid ratio of 1:40 (g·mL-1)was extracted at 70℃ for 40 minutes. The yield of flavonoids was 2.78% with a relative standard deviation of 0.635%. The resulting extract was tested for its antioxidant activity by means of DPPH and ABTS radical scavenging capabilities to show IC50at 1.21 mg·mL-1 and 1.06 mg·mL-1, respectively. It appeared that the current method could render a high yield on flavonoids that showed a substantial antioxidative activity.
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Key words:
- microwave /
- Abelmoschus esculeutus /
- flavonoids /
- extraction /
- antioxidant activity
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表 1 正交试验因素水平
Table 1. Factors and levels of orthogonal design
水平 温度/℃ 提取时间/min 乙醇体积分数/% 料液比/(g·mL-1) 1 60 20 60 1: 30 2 70 30 70 1: 40 3 80 40 80 1: 50 
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表 2 正交试验方案及其结果
Table 2. Designs and results of orthogonal experiment
因素 温度/℃ 提取时间/min 乙醇体积分数/% 料液比/(g·mL-1) 黄酮类化合物提取率/% 1 60 20 60 1: 30 1.9957 2 60 30 70 1: 40 2.4719 3 60 40 80 1: 50 2.1644 4 70 20 70 1: 50 2.5851 5 70 30 80 1: 30 2.4683 6 70 40 60 1: 40 2.7201 7 80 20 80 1: 40 2.3788 8 80 30 60 1: 50 2.0325 9 80 40 70 1: 30 2.2571 k1 2.211 2.320 2.249 2.240 k2 2.591 2.324 2.438 2.524 k3 2.223 2.381 2.337 2.261 R 0.380 0.061 0.189 0.284
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