Bioinformatics of Specific Gene Mmc-3740 of Mycoplasma mycoides subsp. capri
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摘要: 为研究丝状支原体山羊亚种Mmc-3740基因编码蛋白的结构与功能，运用生物信息学软件，对该基因编码蛋白的二级结构、跨膜区域、信号肽等生物信息进行预测及分析。结果显示，该基因编码蛋白拥有α螺旋、延伸链、随机卷曲结构；有信号肽，亚细胞定位在细胞外或者细胞质内，可能为分泌蛋白，具有较高的免疫原性，抗原表位偏高的区域主要集中在氨基酸序列的第28~51、60~67、80~130位处；蛋白分子式为C607H1000N184O261S5，分子量15.21 kD，等电点（PI值）为4.42，在微生物体内半衰期较短，为不稳定蛋白。该结果为Mmc-3740编码蛋白的进一步研究提供基础数据。Abstract: Structure and function of Mmc-3740 encoded protein of Mycoplasma mycoides subsp. capri were studied based on the bioinformatics of the secondary structure, transmembrane region and signal peptide of the gene. The results showed that the protein was an alpha helix with extended random coil, and that the signal peptide located either extracellular or in cytoplasmic with high antigen index and immunogenicity.The area that showed high antigen index and immunogenicity concentrated in the regions of 28-51, 60-67 and 80-130 amino acid sequences.The molecular formula of the protein was C607H1000N184O261S5 with a molecular weight of 15.21 kD and an isoelectric point of 4.42.The half-life of Mmc-3740 in microorganisms appeared to be short and unstable.
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Key words:
- Mycoplasma mycoides subsp.capri /
- Mmc-3740 gene /
- bioinformatics
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图 1 Mmc-3740编码蛋白α螺旋、延伸链、随机卷曲结构分布
Figure 1. Distribution of alpha helix, extension chain and random coil structure of Mmc-3740
表 1 Mmc-3740编码蛋白二级结构预测结果
Table 1. Predicted Mmc-3740 secondary structure
结构 α-螺旋 310-螺旋 π-螺旋 β-折叠桥 延伸链 β-转角 弯曲区 随机卷曲 模糊区 其他 碱基数 30 0 0 0 18 0 0 101 0 0 占比/% 20.13 0.00 0.00 0.00 12.08 0.00 0.00 67.79 0.00 0.00 
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表 2 SOSUI预测结果
Table 2. SOSUI prediction
N端 跨膜区 C端 类型 长度 结果 4 LLTLLGSISIVAS TASVAIACTD 26 一级结构 23 该氨基酸序列是一种膜蛋白，其中有1个跨膜螺旋
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表 3 CELLO预测结果
Table 3. CELLO prediction
CELLO预测定位 数值 细胞外 1.902 * 细胞质 1.295 * 周质 0.993 外膜 0.451 内膜 0.359 注：标有*号为可信值。
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表 4 Mmc-3740编码蛋白氨基酸组成
Table 4. Amino acids of Mmc-3740
氨基酸 数量 占比/% 丙氨酸Ala (A) 7 4.90 精氨酸Arg (R) 2 1.40 天冬酰胺Asn (N) 15 10.40 天冬氨酸Asp (D) 9 6.20 半胱氨酸Cys (C) 1 0.70 谷氨酰胺Gln (Q) 5 3.50 谷氨酸Glu (E) 16 11.10 甘氨酸Gly (G) 7 4.90 组氨酸His (H) 2 1.40 异亮氨酸Ile (I) 6 4.20 亮氨酸Leu (L) 6 4.20 赖氨酸Lys(K) 10 6.90 甲硫氨酸Met (M) 4 2.80 苯丙氨酸Phe (F) 0 0.00 脯氨酸Pro (P) 3 2.10 丝胺酸Ser (S) 32 22.20 苏氨酸Thr (T) 14 9.70 色氨酸Trp (W) 0 0.00 酪氨酸Tyr (Y) 0 0.00 颉氨酸Val (V) 5 3.50 吡咯赖氨酸Pyl (O) 0 0.00 硒半胱氨酸Sec (U) 0 0.00
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