9个新育甜玉米自交系遗传聚类和主要农艺性状配合力分析

张扬; 廖长见; 林建新; 卢和顶; 陈山虎

doi:10.19303/j.issn.1008-0384.2019.01.003

9个新育甜玉米自交系遗传聚类和主要农艺性状配合力分析

doi: 10.19303/j.issn.1008-0384.2019.01.003

福建省农业科学院作物研究所, 福建福州 350013

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2017R1026-1

国家重点研发计划子课题 2018YFD0100106

国家重点研发计划子课题 2018YFD0200707-5

福建省特色旱作物品种选育工程技术研究中心闽科计[2017]13号

福建省财政专项——福建省农业科学院科技创新团队项目 STIT2017-2-6

福建省农业科学院科技创新项目 A2015-1

福建省农业科学院科技创新项目 PC2018-3

福建省农业科学院科技创新项目 AB2017-9

福建省农业科学院对外合作项目 DEC201821213

详细信息

作者简介:
张扬(1985-), 男, 硕士, 助理研究员, 主要从事玉米遗传育种及栽培技术研究(E-mail:zywheat@163.com)

通讯作者:
陈山虎(1962-), 男, 研究员, 主要从事玉米遗传育种及栽培技术研究(E-mail:ymfaas@163.com)

中图分类号: S513
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出版历程
- 收稿日期: 2018-05-28
- 修回日期: 2018-09-20
- 刊出日期: 2019-01-28

Combining Ability on Main Agronomic Traits and Genetic Clustering of Nine New Breeds of Sweet Corns

Crop Research Institute, Fujian Academy of Agricutural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 目的利用遗传聚类和配合力分析来了解新育成9个甜玉米自交系的遗传背景和配合力，为组配新品种提供科学依据。方法以TS01-1、BS02-1、GS132、AS67、SS1等5个白粒自交系为母本，闽甜系688、闽甜系G73、闽甜系197、X90-34等4个高配合力黄粒自交系为父本，按5×4不完全双列杂交设计组配20个杂交组合，并对其株高、穗位高、穗长、穗粗、穗行数、行粒数、百粒重、出籽率、带苞叶穗重和去苞叶穗重等10个性状的配合力进行研究。结果从去苞叶穗重的一般配合力值得出，供试甜玉米自交系的配合力大小顺序，白粒自交系为AS67 > GS132 > TS01-1 > BS02-1 > SS1，黄粒自交系为闽甜系688 > X90-34 >闽甜系G73 >闽甜系197。从特殊配合力值得出，杂交组合AS67×闽甜系688的特殊配合力值最大，实际测产产量也最高。从分子水平对9个亲本的亲缘关系进行聚类分析，将9个亲本分成黄粒和白粒两个群。结论黄、白粒自交系间杂交具有一定的杂种优势；聚类分析结果与试验设计的父母本划分一致。
- 甜玉米 /
- 白色籽粒 /
- 黄色籽粒 /
- 自交系 /
- 配合力 /
- 遗传聚类
Abstract: Objective Genetic backgrounds and combining abilities of 9 newly bred sweet corn inbred lines were studied. Method The inbred lines included 5 white sweet corn female parents P1 (i.e., TS01-1, BS02-1, GS132, AS67 and SS1) and 4 sweet corn male parents P2 (i.e., Mintianxi 688, Mintianxi G73, Mintianxi 197 and X90-34). They were cross-bred into 20 two-tone crosses by a 5×4 incomplete diallel design. The combining ability on 10 traits including plant height, ear location, panicle length, ear girth, ear row count, grains per row, hundred-grain weight, kernel ratio, panicle weight with and without husk, of each hybrid was determined. Result Based on the de-husk panicle weight, the general combining abilities of the white sweet corn inbred lines were in the order of AS67 > GS132 > TS01-1 > BS02-1 > SS1, and those of the yellow sweet corns Mintianxi 688 > X90-34 > Mintianxi G73 > Mintianxi 197. AS67×Mintianxi 688 exhibited the highest specific combining ability with the greatest actual yield among all. The genetic cluster analysis classified the 9 parents into yellow-and white-kernel groups. Conclusion The yellow-kernel and white-kernel sweet corn inbred lines crossed between the 9 parents showed superior heterosis, and the classifications were consistent between the parents and the experimental design.
- sweet corn /
- inbred lines /
- combining ability /
- genetic clustering /

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图 1 9个自交系聚类分析

Figure 1. Neighbor-joining trees of 9 inbred lines based on genetic distance

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表 1 40对核心引物

Table 1. 40 pairs of core primers

编号 Number	标记 Marker
1	bnlg439w1
2	umc1335y5
3	umc2007y4
4	bnlg1940k7
5	umc2105k3
6	phi053k2
7	phi072k4
8	bnlg2291k4
9	umc1705w1
10	bnlg2305k4
11	bnlg161k8
12	bnlg1702k1
13	umc1545y2
14	umc1125y3
15	bnlg240k1
16	phi080k15
17	phi065k9
18	umc1492y13
19	umc1432y6
20	umc1506k12
21	umc1147y4
22	bnlg1671y17
23	phi96100y1
24	umc1536k9
25	bnlg1520k1
26	umc1489y3
27	bnlg490y4
28	umc1999y3
29	umc2115k3
30	umc1429y7
31	bnlg249k2
32	phi299852y2
33	umc2160k3
34	umc1936k4
35	bnlg2235y5
36	phi233376y1
37	umc2084w2
38	umc1231k4
39	phi041y6
40	umc2163w3

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表 2 9个自交系10个主要性状配合力方差分析结果(F值)

Table 2. Analysis of variance on combining ability on yield traits of inbred corns (F-value)

变异来源 Variation source	自由度 Translation freedom	株高 Plant height	穗位高 Ear high	穗长 Panicle length	穗粗 Ear thick	穗行数 Ear rows	行粒数 Grains per row	百粒重 Hundred-grain weight,	出籽率 Kernel ratio	穗重(带苞叶) Panicle weight(with husk)	穗净重 Panicle weight
区组Block	2	3.791	42.569^*	0.674	0.036	2.467	3.791	5.958^**	0.007^**	0.001	0.021
杂交种Hybrids	19	22.697^**	238.348^**	15.079^**	0.171^**	6.018^**	22.697^**	0.267	0.003	0.035^**	0.039
P₁	4	4.368	151.977	31.028	0.373^*	4.167	4.367	1.917^**	0.003	0.026	0.028
P₂	3	0.999	156.923	3.206	0.076	4.867	0.999	0.037	0.003	0.008	0.013
P₁×P₂	12	34.232^**	287.495^*	12.731^**	0.128^**	6.922^**	34.232^**	0.141^*	0.003^*	0.045^**	0.049^*
误差Error	38	5.558	12.004	0.349	0.041	1.344	5.557	0.250	0.001	0.004	0.028
注：、分别表示差异达5%和1%显著水平。 Note: , ** respectively indicate differences significant levels of 5% and 1%.

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表 3 9个自交系主要性状一般配合力(GCA)相对效应值

Table 3. Relative effect of general combining ability(GCA)on yield traits of 9 inbred lines

亲本 Parent	株高 Plant height	穗位高 Ear high	穗长 Panicle length	穗粗 Ear thick	穗行数 Ear rows	行粒数 Grains per row	百粒重 Hundred-grain weight	出籽率 Kernel ratio	穗重(带苞叶) Panicle weight(with husk)	穗净重 Panicle weight
T1	-5.47	2.71	-7.98	1.21	-0.92	-0.23	-1.89	-0.56	-4.81	1.05
T2	2.44	6.18	-7.61	4.12	4.59	-1.41	-1.48	3.50	-0.58	-5.60
T3	1.74	-1.92	4.69	0.44	-1.83	-1.25	9.20	-0.32	3.25	5.90
T4	-2.34	-5.96	10.53	-5.40	-3.67	2.81	-0.66	-2.99	4.27	4.97
T5	3.63	-1.01	0.37	-0.36	1.83	0.08	-5.17	0.37	-2.13	-6.32
M1	-0.32	1.41	-1.94	-1.06	-3.85	-1.06	0.16	2.34	-0.36	2.04
M2	-0.95	-5.69	0.17	1.14	3.49	0.44	-1.81	-2.20	2.10	-2.22
M3	-0.73	-0.05	3.17	-1.34	-0.92	0.50	-0.49	-0.63	-2.31	-3.67
M4	2.00	4.33	-1.40	1.26	1.28	0.13	2.13	0.49	0.57	3.85

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表 4 20个组合主要性状特殊配合力(SCA)相对效应值

Table 4. Estimates on specific combining ability (SCA) on yield traits of 20 hybrids

组合 Hybridized combination	株高 Plant height	穗位高 Ear high	穗长 Panicle length	穗粗 Ear thick	穗行数 Ear rows	行粒数 Grains per row	百粒重 Hundred-grain weight	出籽率 Kernel ratio	穗重(带苞叶) Panicle weight(with husk)	穗净重 Panicle weight
T1×M1	3.03	-3.48	2.34	1.45	-3.49	5.98	1.89	-1.76	4.30	0.51
T1×M2	1.96	-7.34	-1.92	-3.17	0.18	-4.27	10.43	-2.32	-3.85	-0.85
T1×M3	-6.30	0.52	-2.17	0.62	-2.75	5.36	-10.59	3.06	-5.18	1.75
T1×M4	1.31	10.30	1.74	1.10	6.06	-7.08	-1.72	1.02	4.73	-1.41
T2×M1	-2.66	-4.66	-8.61	-3.68	-8.99	-12.53	-8.37	-5.35	-15.06	-13.48
T2×M2	0.22	-11.87	3.00	-0.91	-5.32	6.28	5.09	2.43	3.92	-17.55
T2×M3	3.76	5.81	2.87	5.95	6.42	5.28	8.70	-0.53	11.65	28.73
T2×M4	-1.32	10.72	2.74	-1.36	7.89	0.97	-5.42	3.45	-0.50	2.30
T3×M1	8.56	21.00	-5.42	-0.27	8.44	-6.13	-5.91	3.10	-8.24	-10.55
T3×M2	7.12	13.75	-16.53	6.69	4.77	-7.31	-0.66	3.94	-1.59	-1.76
T3×M3	-6.80	-16.40	8.00	-3.84	-1.83	6.69	1.31	0.97	5.71	6.38
T3×M4	-8.88	-18.36	13.94	-2.58	-11.38	6.75	5.25	-8.01	4.13	5.94
T4×M1	-9.54	-2.10	8.92	1.06	6.61	7.94	2.30	4.38	13.36	11.78
T4×M2	-5.73	-1.59	9.68	0.36	-0.73	-1.69	-0.66	-4.51	3.71	8.69
T4×M3	9.24	5.11	-12.58	-5.39	-11.01	-15.19	2.96	-1.00	-16.88	-21.55
T4×M4	6.03	-1.43	-6.02	3.97	5.14	8.94	-4.60	1.13	-0.19	1.09
T5×M1	0.61	-10.77	2.76	1.45	-2.57	4.73	10.10	-0.37	5.65	11.74
T5×M2	-3.57	7.04	5.76	-2.97	1.10	6.98	-14.20	0.46	-2.19	11.48
T5×M3	0.11	4.95	3.87	2.65	9.17	-2.14	-2.38	-2.50	4.71	-15.30
T5×M4	2.85	-1.23	-12.40	-1.13	-7.71	-9.58	6.49	2.41	-8.17	-7.92

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