Breeding of High-yield, High-starch Cassava, Guire No. 11
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摘要:
目的 选育高产、高淀粉、广适性的鲜食加工兼用型双高木薯杂交新品种。 方法 以高产低粉的新选048为母本,高产高粉、低氢氰酸的桂热891为父本,采用人工开花调控技术进行诱导并杂交获得F1代,经单株评选试验、各级系比试验和生产性试验,鉴定、筛选出优良品种桂热11号。 结果 在生产性试验中,桂热11号平均鲜薯产量39.68 t·hm−2,分别比对照华南205(SC205)和华南9(SC9)增产28.38%、52.03%,其鲜薯淀粉含量32.4%,比对照SC205高3.7百分点,与SC9相持平;桂热11号干物质率40.70%,比SC205高4.7个百分点;氢氰酸含量低,平均为22.2 mg·kg−1 。桂热11号鲜薯含蛋白质0.75 g·hg−1 、总糖1.50 g·hg−1 、粗纤维1.00%、维生素C 37.20 mg·hg−1、胡萝卜素123 μg·hg−1,于2020年11月3日通过“热带作物品种”审定(热品审2020002)。 结论 桂热11号高产高淀粉,氢氰酸含量低,适应性广,是丰产稳产性好的鲜食加工兼用型优良品种,具有耐旱耐寒和较抗螨、不易落叶等特点,适合在广西、海南和江西等木薯主产区推广种植。 Abstract:Objective A high-yield, high-starch, wide-adaptability cassava cultivar was bred in Guangxi province for fresh consumption or further processing. Method A newly bred F1 progeny of crossing between the high-yield, low-starch Xinxuan 048 as female parent and the high-yield, high-starch, 1ow-hydrocyanic acid Guire 891 as male parent was generated by artificial pollination and induction. After seedling selection and a series of primary, intermediate, and advanced tests as well as regional and production trials, Guire No. 11 (GR11) was selected. Result As shown in the production trial, GR11 delivered an average tuber yield of 39.68 t·hm−2, which was 28.38% and 52.03% higher than SC No. 205 and SC No. 9, respectively. The tubers had a starch content of 32.4%, which was 3.7% higher than SC No. 205 and same as SC No. 9; a dry matters content of 40.7%, which was 4.7% higher than SC No. 205; an HCN content of 22.2 mg·kg−1 (based on FW); a rich content of nutrients (based on FW), such as 0.75 g·hg−1 on protein, 1.5 g·hg−1 on total sugar, 1.0% on crude fiber, 37.2 mg·hg−1 on vitamin C, and 123 μg·hg−1 on carotene. The new cultivar received the tropical crop variety certification on November 3, 2020. Conclusion The high and stable yield GR11 with wide adaptability was resistant to drought, cold, and mites, and its leaves did not fall off easily. It could be adequately cultivated in current cassava-producing areas in Guangxi, Hainan, and Jiangxi provinces for either fresh consumption, further processing, or as a raw material for manufacturing starch products. -
Key words:
- Cassava /
- Guire No.11 /
- yield /
- quality /
- breeding selection
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表 1 2015–2017年GR11高级系比试验
Table 1. Advanced performance trail results on GR11 in 2015-2017
年份
Year品种
Variety鲜薯产量
Fresh root yield/(t·hm−2)比对照增产
Increasing rate/%淀粉含量
Starch content/%Ⅰ Ⅱ Ⅲ 平均
AverageSC205
(CK)SC9
(CK)Ⅰ Ⅱ Ⅲ 平均
Average2015 GR11 33.91 39.50 35.86 36.42±1.6 a 86.31 71.48 26.3 25.3 27.0 26.2±0.5 a SC205(CK) 20.80 18.60 19.25 19.55±0.7 b − − 20.7 20.5 19.0 20.1±0.5 b SC9(CK) 21.39 20.34 21.99 21.24±0.5 b − − 27.1 28.1 27.6 27.6±0.3 a 2016 GR11 36.40 35.42 48.12 39.98±4.1 a 92.12 76.20 29.0 28.3 29.8 29.0±0.4 a SC205(CK) 21.53 19.79 21.10 20.81±0.5 b − − 25.8 25.6 26.0 25.8±0.1 c SC9(CK) 21.80 23.05 23.23 22.69±0.4 b − − 27.0 26.8 27.5 27.1±0.2 b 2017 GR11 37.90 38.27 42.35 39.51±1.4 a 38.52 72.74 27.4 28.9 30.9 29.1±1.0 a SC205(CK) 24.42 29.87 31.26 28.52±2.1 b − − 26.5 25.9 26.5 26.3±0.2 b SC9(CK) 24.82 23.46 20.34 22.87±1.3 b − − 26.7 27.2 26.4 26.8±0.2 b 注:(1)表中不同小写字母表示处理间差异显著(P<0.05)。(2)“-”表示此项无。
Note: (1)Different lowercase letters in the table indicate significant differences at 0.05 levels in Duncan test.(2)"-"indicates that this item is absent.表 2 2017–2018年GR11区域性试验
Table 2. Regional trail results on GR11 in 2017–2018
年份
Year试验地点
Site鲜薯产量
Fresh root yield/(t·hm−2)比对照增产
Increasing rate/%淀粉含量
Starch content/%GR11 SC205(CK) SC9(CK) SC205(CK) SC9(CK) GR11 SC205(CK) SC9(CK) 2017 南宁 Nanning 39.36 19.68 22.87 100.00 72.10 29.1 26.2 26.8 2018 南宁 Nanning 40.47 28.52 23.36 41.90 73.24 29.4 26.3 27.7 平均 Average 39.92 24.10 23.12 70.95 72.67 29.3 26.3 27.3 2017 武鸣 Wuming 42.46 39.59 29.20 7.25 45.41 27.9 25.3 27.6 2018 武鸣 Wuming 41.51 37.23 26.38 11.50 57.35 29.2 26.5 28.6 平均 Average 41.99 38.41 27.79 9.37 51.38 28.6 25.9 28.1 2017 桂平 Guiping 41.30 34.43 23.34 19.95 76.95 30.0 27.2 29.7 2018 桂平 Guiping 41.61 37.51 21.53 10.93 93.27 28.4 26.6 28.3 平均 Average 41.46 35.97 22.44 15.44 85.11 29.2 26.9 29.0 2017 南昌 Nanchang 43.54 39.47 24.73 10.31 76.06 29.9 27.8 29.2 2018 南昌 Nanchang 42.27 38.19 27.73 10.68 52.43 30.2 28.2 28.5 平均 Average 42.91 38.83 26.23 10.50 64.25 30.1 28.0 28.9 2017 白沙 Baisha 39.29 35.39 28.33 11.02 38.69 28.5 26.2 28.0 2018 白沙 Baisha 40.38 36.22 28.31 11.49 42.64 29.1 25.9 27.8 平均 Average 39.84 35.81 28.32 11.25 40.66 28.8 26.1 27.9 表 3 2018–2019年GR11生产性试验
Table 3. Production trail results on GR11 in 2018–2019
年份
Year试验点
Site鲜薯产量
Fresh root yield/(t·hm−2)比对照增产
Increasing rate/%GR11 SC205(CK) SC9(CK) SC205(CK) SC9(CK) 2018 南宁 Nanning 40.76 28.28 22.02 44.13 85.10 2019 南宁 Nanning 42.24 31.17 31.95 35.51 32.21 平均 Average 41.50 29.73 26.99 39.82 58.66 2018 武鸣 Wuming 42.36 38.68 24.86 9.51 70.39 2019 武鸣 Wuming 41.78 32.04 32.82 30.40 27.30 平均 Average 42.07 35.36 28.84 19.96 48.85 2018 桂平 Guiping 36.39 32.52 22.34 11.90 62.89 2019 桂平 Guiping 39.38 32.07 29.81 22.79 32.10 平均 Average 37.89 32.30 26.08 17.35 47.50 2018 南昌 Nanchang 41.73 29.06 22.02 43.60 89.51 2019 南昌 Nanchang 27.60 17.55 17.37 57.26 58.89 平均 Average 34.67 23.31 19.70 50.43 74.20 2018 白沙 Baisha 41.19 35.21 27.95 16.98 47.37 2019 白沙 Baisha 43.34 32.48 29.84 33.44 45.24 平均 Average 42.27 33.85 28.90 25.21 46.31 表 4 2018–2019年GR11与对照SC205的淀粉和干物质率对比结果
Table 4. Contents of starch and dry matters of GR11 and SC205 tested in 2018–2019
年份
Year试验点
Site淀粉含量
Starch content/(g·hg−1 )干物质率
Dry matter content/%氢氰酸
HCN/(mg·kg−1 )GR11 SC205(CK) GR11 SC205(CK) GR11 SC205(CK) 2018 南昌 Nanchang 30.3 26.9 39.6 34.6 20.5 - 2019 桂平 Guiping 34.5 30.5 41.8 37.5 23.9 - 注:“-”表示未测定,下同。
Note: “-” indicates that this item is not measured, the same below.表 5 2015–2019年GR11鲜薯产量及淀粉含量
Table 5. Tuber yield and starch content of GR11 tested in 2015-2019
年份
Year鲜薯产量
Fresh root yield/(t·hm−2)比对照增产
Increasing rate/%淀粉含量
Starch content/%GR11 SC205(CK) SC9(CK) SC205(CK) SC9(CK) GR11 SC205(CK) SC9(CK) 2015 36.42 19.55 21.24 86.29 71.47 26.2 20.1 27.6 2016 39.98 20.81 22.69 92.12 76.20 29.0 25.8 27.1 2017 40.35 31.12 24.28 29.68 66.19 29.1 26.0 27.2 2018 40.77 34.14 24.65 19.42 65.40 29.8 26.8 - 2019 38.87 29.06 28.36 33.76 37.06 34.5 30.5 - 平均Average 39.28 26.94 24.24 45.83 62.01 29.7 25.8 27.3 表 6 桂热11号分子指纹模式图谱
Table 6. Molecular fingerprints of GR11
编号
Number品种
Variety引物SSR6
Primer SSR6引物SSR10
Primer SSR10a b a b 1 SC9 ◆ ◆ − ◆ 2 SC205 − ◆ ◆ − 3 GR891 − ◆ ◆ − 4 NZ199 − ◆ ◆ − 5 XX048 ◆ ◆ ◆ ◆ 6 GR11 − ◆ ◆ ◆ 7 GR10 − ◆ − ◆ 8 561 ◆ ◆ − ◆ 注:“◆”表示该位点有特征条带,“−”表示该位点无条带。
Note: “◆” indicates loci with characteristic bands; “−” loci without bands.表 7 2017–2019年GR11抗逆性表现
Table 7. Stress resistance of GR11 shown in 2017–2019
抗逆性
Stress resistance2017 2018 2019 GR11 SC205 SC9 GR11 SC205 SC9 GR11 SC205 SC9 耐寒性 Cold tolerance S M M S M M S M M 耐旱性 Drought tolerance S M M S M M S M M 细菌性枯萎病抗性 Resistance to bacterial blight S R R S R R S R R 朱砂叶螨抗性 Resistance to tetranychus cinnabarinus MR S R MR S R MR S R 注: (1)M-耐寒性、耐旱性中等;(2)S-耐寒性、耐旱性强;(3)S-细菌性枯萎病、朱砂叶螨感病;(4)R-抗细菌性枯萎病、朱砂叶螨病;(5)MR-中抗细菌性枯萎病、朱砂叶螨。
Note: (1) M: moderate cold and drought tolerance. (2) S: strong cold and drought tolerance. (3) S: susceptible to bacterial blight and Tetranychus cinnabarinus infestation. (4) R: resistant to bacterial blight and T. cinnabarinus infestation. (5) MR: moderately resistant to bacterial blight and T. cinnabarinus infestation. -
[1] 闫庆祥, 李开绵, 黄洁, 等. 木薯种质资源主要性状描述与数据采集研究 [J]. 安徽农业科学, 2013, 41(26):10600−10601, 10639. doi: 10.3969/j.issn.0517-6611.2013.26.013YAN Q X, LI K M, HUANG J, et al. Description and data collection in main traits of cassava germplasm resource [J]. Journal of Anhui Agricultural Sciences, 2013, 41(26): 10600−10601, 10639.(in Chinese) doi: 10.3969/j.issn.0517-6611.2013.26.013 [2] 张振文, 古碧. 9个木薯品种酒精加工特性综合评价 [J]. 西南农业学报, 2014, 27(2):807−812.ZHANG Z W, GU B. Comprehensive assessment of ethanol processing characteristics for cassava [J]. Southwest China Journal of Agricultural Sciences, 2014, 27(2): 807−812.(in Chinese) [3] FAO. 联合国粮农组织统计数据库[EB/OL]. [2019-08-02]. http://www.fao.org/faostat/zh/#data. [4] 叶剑秋, 李开绵, 陈丽珍, 等. 木薯新品种华南7号的选育 [J]. 热带作物学报, 2007(01):24−29. doi: 10.3969/j.issn.1000-2561.2007.01.006YE J Q, LI K M, CHEN L Z, et al. Breeding and selection of new cassava variety SC7 [J]. Chinese Journal of Tropical Crops, 2007(01): 24−29.(in Chinese) doi: 10.3969/j.issn.1000-2561.2007.01.006 [5] 叶剑秋, 黄洁, 陈丽珍, 等. 木薯新品种华南8号的选育 [J]. 热带作物学报, 2006, 27(4):19−24. doi: 10.3969/j.issn.1000-2561.2006.04.005YE J Q, HUANG J, CHEN L Z, et al. Breeding of a new cassava variety SC 8 [J]. Chinese Journal of Tropical Crops, 2006, 27(4): 19−24.(in Chinese) doi: 10.3969/j.issn.1000-2561.2006.04.005 [6] 叶剑秋, 郑永清, 薛茂富, 等. 木薯新品种‘华南10号’的选育 [J]. 热带作物学报, 2011, 32(10):1799−1803. doi: 10.3969/j.issn.1000-2561.2011.10.002YE J Q, ZHENG Y Q, XUE M F, et al. Breeding of the new cassava cultivar SC 10 [J]. Chinese Journal of Tropical Crops, 2011, 32(10): 1799−1803.(in Chinese) doi: 10.3969/j.issn.1000-2561.2011.10.002 [7] 叶剑秋, 王明, 肖鑫辉, 等. 木薯新品种华南13号的选育 [J]. 热带农业工程, 2017, 41(4):1−8.YE J Q, WANG M, XIAO X H, et al. Breeding of a new cassava cultivar SC No. 13 [J]. Tropical Agricultural Engineering, 2017, 41(4): 1−8.(in Chinese) [8] 卢诚, 陈新, 周新成, 等. 木薯新品种‘华南16号’的选育 [J]. 热带作物学报, 2020, 41(9):1756−1761. doi: 10.3969/j.issn.1000-2561.2020.09.005LU C, CHEN X, ZHOU X C, et al. Breeding of a new cassava cultivar ‘South China No. 16’ [J]. Chinese Journal of Tropical Crops, 2020, 41(9): 1756−1761.(in Chinese) doi: 10.3969/j.issn.1000-2561.2020.09.005 [9] 林雄, 李开绵, 黄洁, 等. 木薯新品种华南5号选育报告 [J]. 热带农业科学, 2001, 21(5):15−19. doi: 10.3969/j.issn.1009-2196.2001.05.004LIN X, LI K M, HUANG J, et al. The breeding report of a new cassava variety SC NO. 5 [J]. Chinese Journal of Tropical Agriculture, 2001, 21(5): 15−19.(in Chinese) doi: 10.3969/j.issn.1009-2196.2001.05.004 [10] 罗兴录. 木薯新品种新选048选育与应用 [J]. 中国农学通报, 2009, 25(24):501−505.LUO X L. Report on applying and breeding of new cassava cultivar Xinxuan 048 [J]. Chinese Agricultural Science Bulletin, 2009, 25(24): 501−505.(in Chinese) [11] 李军, 田益农. 木薯新品种GR911的选育 [J]. 广西热作科技, 1999(4):1−4.LI J, TIAN Y N. Breeding of a new cassava variety GR911 [J]. Guangxi Science & Technology of Tropical Crops, 1999(4): 1−4.(in Chinese) [12] 李军, 田益农, 盘欢, 等. 木薯品种桂热4号的选育及栽培要点 [J]. 南方农业学报, 2014, 45(7):1183−1187. doi: 10.3969/j:issn.2095-1191.2014.7.1183LI J, TIAN Y N, PAN H, et al. Breeding of a new cassava variety GR4 and its cultivation technologies [J]. Journal of Southern Agriculture, 2014, 45(7): 1183−1187.(in Chinese) doi: 10.3969/j:issn.2095-1191.2014.7.1183 [13] 黄洁, 单荣芝, 李开绵, 等. 优质鲜食木薯新品种华南9号 [J]. 中国热带农业, 2006(5):47−48. doi: 10.3969/j.issn.1673-0658.2006.05.030HUANG J, SHAN R Z, LI K M, et al. A new high-quality and fresh-edible cassava variety SC No. 9 [J]. China Tropical Agriculture, 2006(5): 47−48.(in Chinese) doi: 10.3969/j.issn.1673-0658.2006.05.030 [14] 田益农, 李军, 盘欢. 木薯新品种GR891的选育 [J]. 广西农业科学, 1998, 29(3):124−125.TIAN Y N, LI J, PAN H. Breeding of a new cassava variety GR891 [J]. Guangxi Agricultural Science, 1998, 29(3): 124−125.(in Chinese) [15] 曹升, 陈江枫, 黄富宇, 等. 广西木薯产业现状分析及其发展建议 [J]. 南方农业学报, 2021, 52(6):1468−1476. doi: 10.3969/j.issn.2095-1191.2021.06.005CAO S, CHEN J F, HUANG F Y, et al. Development status and countermeasures of cassava industry in Guangxi [J]. Journal of Southern Agriculture, 2021, 52(6): 1468−1476.(in Chinese) doi: 10.3969/j.issn.2095-1191.2021.06.005 [16] 叶剑秋, 黄洁, 陈松笔, 等. 木薯新品种华南12号的选育 [J]. 热带作物学报, 2014, 35(11):2121−2128. doi: 10.3969/j.issn.1000-2561.2014.11.004YE J Q, HUANG J, CHEN S B, et al. Breeding of a new cassava cultivar SC No. 12 [J]. Chinese Journal of Tropical Crops, 2014, 35(11): 2121−2128.(in Chinese) doi: 10.3969/j.issn.1000-2561.2014.11.004 [17] 中华人民共和国农业部.热带作物品种区域试验技术规程 木薯: NY/T 2446—2013[S].北京: 中国标准出版社, 2013. [18] 赵大伟, 郭元友, 邓国军, 等. 木薯间作糯玉米的高产高效模式分析 [J]. 热带农业科学, 2017, 37(4):1−6,12.ZHAO D W, GUO Y Y, DENG G J, et al. Analysis of high yield and efficient intercropping patterns of cassava with waxy corn [J]. Chinese Journal of Tropical Agriculture, 2017, 37(4): 1−6,12.(in Chinese) [19] 韦本辉, 甘秀芹, 刘斌, 等. 桂木薯1号等木薯新品种的选育及栽培技术 [J]. 南方农业学报, 2016, 47(7):1088−1094. doi: 10.3969/j:issn.2095-1191.2016.07.1088WEI B H, GAN X Q, LIU B, et al. Breeding and cultivation techniques of Guimushu 1 and other cassava varieties [J]. Journal of Southern Agriculture, 2016, 47(7): 1088−1094.(in Chinese) doi: 10.3969/j:issn.2095-1191.2016.07.1088 [20] 卢昕, 李超萍, 时涛, 等. 国内木薯主产区细菌性枯萎病病原鉴定 [J]. 广东农业科学, 2013, 40(21):84−87,2. doi: 10.3969/j.issn.1004-874X.2013.21.023LU X, LI C P, SHI T, et al. Pathogen identification of cassava bacterial blight from several main cultivation area in China [J]. Guangdong Agricultural Sciences, 2013, 40(21): 84−87,2.(in Chinese) doi: 10.3969/j.issn.1004-874X.2013.21.023 [21] 陈青, 卢芙萍, 黄贵修, 等. 木薯害虫普查及其安全性评估 [J]. 热带作物学报, 2010, 31(5):819−827. doi: 10.3969/j.issn.1000-2561.2010.05.023CHEN Q, LU F P, HUANG G X, et al. General survey and safety assessment of cassava pests [J]. Chinese Journal of Tropical Crops, 2010, 31(5): 819−827.(in Chinese) doi: 10.3969/j.issn.1000-2561.2010.05.023