茄子果实生长发育过程中果皮主要色素含量的变化及相关基因表达分析

谭枫; 肖凯; 刘晓慧; 黄倩茹; 张爱冬; 吴雪霞

doi:10.19303/j.issn.1008-0384.2023.02.005

茄子果实生长发育过程中果皮主要色素含量的变化及相关基因表达分析

doi: 10.19303/j.issn.1008-0384.2023.02.005

谭枫^{1, 2,},
肖凯²,
刘晓慧^{1, 2},
黄倩茹²,
张爱冬²,
吴雪霞^2, ,

1.
上海海洋大学水产与生命学院，上海　201306
2.
上海市农业科学院园艺研究所，上海　201403

基金项目: 上海市“科技创新行动计划”农业领域项目（21N51900100）；国家大宗蔬菜产业技术体系项目（CARS-25）；上海市农业科学院卓越团队项目[沪农科卓（2022）019]

详细信息

作者简介:
谭枫（1998−），男，硕士研究生，研究方向：茄子分子植物育种（E-mail：961631817@qq.com）

通讯作者:
吴雪霞（1978−），女，研究员，研究方向：茄子种质创新、新品种选育和分子生物学技术（E-mail：wuxuexia@sina.com）

中图分类号: S641
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出版历程
- 收稿日期: 2022-04-05
- 修回日期: 2022-12-20
- 网络出版日期: 2023-03-28
- 刊出日期: 2023-02-28

Formation and Gene Expression of Pigments in Eggplant Pericarp

TAN Feng^{1, 2
,},
XIAO Kai²,
LIU Xiaohui^{1, 2},
HUANG Qianru²,
ZHANG Aidong²,
WU Xuexia^{2
, ,}

1.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai　201306, China
2.
Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai　201403, China

摘要

摘要: 目的探讨茄子果实生长发育过程中果皮主要色素含量变化及色素合成过程中相关基因的表达，为茄子生长发育过程中果皮颜色变化机理研究奠定基础。方法以2份高代自交系茄子（绿色果皮启董和紫黑色果皮8果）为试验材料，分别于授粉后15、20、25、30、35 d进行花青素相对含量、叶绿素和类胡萝卜素含量的测定，同时对花青素生物合成结构基因PAL、CHS、CHI、F3H、DFR、ANS及转录因子MYB75、JAF13、WD40，叶绿素合成关键基因HEMA1, 类胡萝卜素合成关键基因PSY1进行表达分析。结果启董茄子的花青素相对含量、叶绿素和类胡萝卜素含量均呈先升高后降低的趋势，分别在授粉后20 、25 、20 d达到峰值；8果茄子的花青素相对含量呈先升高再降低的变化趋势，在25 d时达到峰值，叶绿素和类胡萝卜素含量一直升高。与启董相比，8果茄子的花青素相对含量较高，叶绿素含量在15～25 d较低，类胡萝卜素含量在30～35 d较高。启董中花青素合成结构基因和转录因子MYB75、JAF13、WD40在不同生长发育时期的表达相较于8果总体偏低且变化趋势相对稳定。8果中花青素合成相关结构基因的表达均在20 d达到顶峰，随后开始降低，转录因子MYB75、WD40表达量整体呈现先升高后降低的趋势，这与花青素相对含量的变化趋势一致。启董和8果中HEMA1、PSY1的表达量均与叶绿素、类胡萝卜素含量的变化趋势存在差异。结论生长发育过程中，主要色素的含量及变化趋势在绿色果皮茄子启董和紫黑色果皮茄子8果之间差异明显，并且花青素、叶绿素和类胡萝卜素相关基因的表达模式不同，可进一步通过遗传学方法挖掘导致果色差异的主要基因。
- 茄子 /
- 果皮颜色 /
- 花青素 /
- 叶绿素 /
- 类胡萝卜素
Abstract: Objective Changes in contents of main pigments and the expressions of related genes in pericarp of eggplant fruit during growth and development were studied concerning the color formation. Method Contents of anthocyanin, chlorophyll, and carotenoid in the fruits of two high-generation eggplant inbred lines, green Qidong and purple-black 8 guo, were measured at 15, 20, 25, 30, and 35 d after pollination. Expressions of the structural genes PAL, CHS, CHI, F3H, DFR, and ANS and the transcription factors MYB75, JAF13, and WD40 related to anthocyanin synthesis, as well as the key gene, HEMA1, in chlorophyll synthesis and PSY1 in carotenoid synthesis were analyzed. Result The contents of 3 categories of pigments in Qidong increased initially followed by a decline during the test period with peaks on the 20 d on anthocyanin, 25 d on chlorophyll, and 20 d on carotenoid. For 8 guo, the anthocyanin peaked on the 25 d, but chlorophyll and carotenoid continuously increased throughout the fruit development. 8 guo had higher relative contents of anthocyanin, lower chlorophyll from 15 d to 25 d, and higher carotenoid between 30 d and 35 d than Qidong. The expressions of anthocyanin synthesis structural genes and transcription factors MYB75, JAF13 and WD40 in Qidong were generally lower and relatively stable than those in 8 guo at different growth and development stages. The expression of anthocyanin synthesis structural genes in 8 guo reached a maximum on the 20 d and then began to decrease, while the expression of transcription factors MYB75 and WD40 rose at first and then decreased, which was similar to the trend observed on the anthocyanin. The expressions of HEMA1 and PSY1 in Qidong and 8 guo differed in the changing patterns of chlorophyll and carotenoid. Conclusion During growth and development, the two eggplant varieties of different colored skins underwent significantly different changes on the 3 pigments. The expressions of the pigment-related genes differed significantly as well, which could be studied from a genetic perspective to reveal the underline mechanisms of the observed color formation and differentiation.
- Solanum melongena L. /
- pericarp color /
- anthocyanin /
- chlorophyll /
- carotenoid

HTML全文

图 1 启董和8果果实不同发育时期

Figure 1. Qidong and 8 guo fruits at developmental stages

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图 2 果实发育时期单果平均质量、横径和纵径的变化

不同字母表示两个品种同一指标差异显著（P＜0.05）。下同。

Figure 2. Related properties of eggplant at developmental stages

Different letters indicate significant difference on same index between two varieties at 0.05 level. Same for below.

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图 3 果实发育过程中果皮色素含量的变化

Figure 3. Changes on pigment contents in pericarp during fruit development

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图 4 色素合成相关基因及转录因子的荧光定量引物验证

Figure 4. Validation on fluorescence quantitative primers of genes and pigment synthesis-related transcription factors

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图 5 色素合成相关基因及转录因子的相对表达量

Figure 5. Relative expressions of genes and pigment synthesis-related transcription factors

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表 1 茄子果皮中色素合成相关基因qRT-PCR引物

Table 1. qRT-PCR primer of pigment synthesis-related gene in eggplant pericarp

引物名称Primer name	序列（5′ to 3′）Sequence (5′ to 3′)
APRT-FAPRT-RPSY1-FPSY1-RHEMA1-FHEMA1-RPAL-FPAL-RCHS-FCHS-RCHI-F	GTGACCGTGCACTTGTGGTAGATGATGGCAACTCAATTACACATGCACACTTAACCGCAGCTAGTAGATTCCCCAGGAGGCACAAGAGATTTTGCATACGCCGTTGATGATCTTAGTCGTGGTTGTCTCGAGGCTGAACATCCTATTTCTGAAATCGCAATGGCCTCTTACCGCCACGAGATAGGTTGATGACATGGCAATATGTCAAGTGCTTGTGTTTAAGCAGCAACACTGTGGAGGACATTGTCAGAAGCAGTGTTGGATTCC
CHI-R	GATAGCCCACTTCCTGGTTTTTCAT
F3H-FF3H-RDFR-FDFR-RANS-FANS-RMYB75-FMYB75-RJAF13-FJAF13-RWD40-FWD40-R	GGTGAACTCGGATAGCAGCAGATTAATGTTATGGGCTCATCCATTATTGCCCCTTTTCTACCCGAAGCACTCTAGATTTCACCATTGGTCAACTGTCCTGCTACCTGAGACTGTCAATGAGGCTGCTATTTAGATTCTTCGGCAGGAACATAACAAGTGACAAGCAAACTACCGATGCACATTGTTCTTGTTGCATGGAGTTTCAATATGATCGATAAGGAAGTCTCGATTGTTCCAACTGCTACTGTTTTCGATGCCTGTGGCTGAGTTGGAAAGCGTTGTGCAGCAGACCACTGAATT

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