基于形态标记与SRAP标记的莱豆种质资源遗传多样性分析

郭媛贞; 黄强; 叶新如; 陈芝; 黄枝; 陈海玲; 颜墩炜; 薛珠政

doi:10.19303/j.issn.1008-0384.2023.02.006

基于形态标记与SRAP标记的莱豆种质资源遗传多样性分析

doi: 10.19303/j.issn.1008-0384.2023.02.006

1.
莆田市农业科学研究所，福建　莆田　351100
2.
福建省农业科学院作物研究所，福建　福州　350013

基金项目: 福建省科技计划引导性项目（2020N0044）

详细信息

作者简介:
郭媛贞（1968−），女，硕士，副研究员，研究方向：蔬菜育种、栽培（E-mail：ptnksgyz@163.com）

通讯作者:
薛珠政（1971−），男，研究员，研究方向：蔬菜育种与栽培技术研究（E-mail：369654817@qq.com ）

中图分类号: S643
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-01
- 修回日期: 2022-11-21
- 网络出版日期: 2023-03-28
- 刊出日期: 2023-02-28

Morphological and SRAP Markers-based Genetic Diversity Determination on Phaseolus lunatus L. Germplasms

1.
Putian Institute of Agricultural Science, Putian, Fujian　351100, China
2.
Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

摘要

摘要: 目的探明莱豆种质资源遗传多样性和亲缘关系，为莱豆种质资源优良基因深度发掘和新品种选育提供科学依据。方法利用形态标记和SRAP分子标记两种方法对22份莱豆资源的26个数量性状和 18个质量性状进行测定、分析。结果筛选出的28对SRAP引物扩增多态性条带158条，平均多态性比率为77.75%。两种标记方法聚类结果显示，根据莱豆荚果大小可以将22份莱豆资源分为三大类群体。其中，“上横山10-2-6”和“下横山10-3-3”亲缘关系较近，推测可能存在频繁的基因交流。结论 22份莱豆资源遗传多样性丰富，形态标记和SRAP分子标记两种聚类方法基本支持根据荚果大小划分莱豆资源，为莱豆种质资源的创新利用奠定基础。
- 莱豆 /
- 形态标记 /
- SRAP分子标记 /
- 遗传多样性 /
- 亲缘关系
Abstract: Objective Genetic diversity and relationship of Phaseolus lunatus L. germplasms were determined using the morphological and SRAP markers. Method Twenty-six quantitative and 18 quality traits of 22 lima bean germplasms were analyzed based on the morphological as well as the SRAP molecular markers. Result Twenty-eight pairs of SRAP primers were selected to obtain 158 amplified polymorphic bands with an average polymorphism ratio of 77.75%. The two methods applying different markers clustered the 22 varieties into 3 categories. Genetically, Shanghengshan10-2-6 and Xiahengshan 10-3-3 were relatively close. They might have gone through numerous gene exchanges in the past. Conclusion The 22 lima bean germplasms were rich in genetic diversity. The morphological and SRAP molecular markers-based clustering essentially agreed with the classification by the pod size.
- Phaseolus lunatus L. /
- morphological markers /
- SRAP molecular markers /
- genetic diversity /
- genetic relationship

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图 1 莱豆农艺性状的聚类分析

Figure 1. Cluster analysis on agronomic traits of P. lunatus

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图 2 SRAP引物对莱豆的扩增结果

Figure 2. Amplified primer

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图 3 22份莱豆聚类图

Figure 3. Dendrogram of 22 P. lunatus germplasms

下载: 全尺寸图片幻灯片

表 1 供试莱豆种质资源

Table 1. P. lunatus germplasms studied

编号 Code	材料名称 Name	原产地 Orign	编号 Code	材料名称 Name	原产地 Orign
1	铁灶本	莆田市城厢区霞林办铁灶村	12	DJ-11-3-1-1	莆田市仙游县大济镇
2	莆1046	莆田市荔城区新度镇渠桥村	13	FT-08-2-5-3	莆田市仙游县枫亭镇
3	JD-1603	莆田市荔城区黄石镇清前村	14	DW17-1-5	莆田市仙游县度尾镇
4	莆莱1号	莆田市农科所选育	15	14LD-5-2-7	莆田市仙游县赖店镇
5	龙莱1号	龙岩市龙津作物所选育	16	DW15-6-1	莆田市仙游县度尾镇
6	QC12-1-17-3	莆田市荔城区新度镇青垞村	17	小莱豆16-5-6	莆田市城厢区常太镇
7	QC12-2-6-5	莆田市荔城区新度镇青垞村	18	小莱豆16-21-11	莆田市城厢区常太镇
8	横山10-1—1	莆田市荔城区新度镇横山村	19	中莱16-12-5	莆田市新度镇渠桥村
9	上横山10-2-6	莆田市荔城区新度镇横山村	20	武平黑花籽扁豆	龙岩市武平县
10	下横山10-3-3	莆田市荔城区新度镇横山村	21	PLQQ-08-2-6	莆田市荔城区新度镇渠桥村
11	新周13-6-2-7	莆田市仙游县园庄镇新周村	22	PLQQ-08-5-3	莆田市荔城区新度镇渠桥村

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表 2 SRAP分析所用的引物序列

Table 2. Primer sequences used in SRAP analysis

编号Code	上游引物序列（5′-3′）Upstream primer sequences	下游引物序列（5′-3′）Downstream primer sequences
me1em20	TGAGTCCAAACCGGATA	GACTGCGTACGAATTCAG
me2em13	TGAGTCCAAACCGGAGC	GACTGCGTACGAATTGGT
me2em20	TGAGTCCAAACCGGAGC	GACTGCGTACGAATTCAG
me4em2	TGAGTCCAAACCGGACC	GACTGCGTACGAATTTGC
me4em4	TGAGTCCAAACCGGACC	GACTGCGTACGAATTTGA
me4em11	TGAGTCCAAACCGGACC	GACTGCGTACGAATTTCG
me5em2	TGAGTCCAAACCGGAAG	GACTGCGTACGAATTTGC
me5em8	TGAGTCCAAACCGGAAG	GACTGCGTACGAATTCTG
me5em13	TGAGTCCAAACCGGAAG	GACTGCGTACGAATTGGT
me6em2	TGAGTCCAAACCGGTAA	GACTGCGTACGAATTTGC
me6em3	TGAGTCCAAACCGGTAA	GACTGCGTACGAATTGAC
me7em12	TGAGTCCAAACCGGTCC	GACTGCGTACGAATTGTC
me8em2	TGAGTCCAAACCGGTGC	GACTGCGTACGAATTTGC
me8em4	TGAGTCCAAACCGGTGC	GACTGCGTACGAATTTGC
me8em11	TGAGTCCAAACCGGTGC	GACTGCGTACGAATTTCG
me10em2	TGGGGACAACCCGGCTT	GACTGCGTACGAATTTGC
me10em3	TGGGGACAACCCGGCTT	TGAGTCCAAACCGGAAT
me10em4	TGGGGACAACCCGGCTT	TGAGTCCAAACCGGACC
me11em4	TGGGGACAACCCGGTTC	TGAGTCCAAACCGGACC
me11em7	TGGGGACAACCCGGTTC	GACTGCGTACGAATTATG
me12em4	TGGGGACAACCCGGTAG	GACTGCGTACGAATTTGA
me13em1	TGGGGACAACCCGGTCA	GACTGCGTACGAATTAAT
me13em3	TGGGGACAACCCGGTCA	TGAGTCCAAACCGGAAT
me13em9	TGGGGACAACCCGGTCA	GACTGCGTACGAATTACG
me13em14	TGGGGACAACCCGGTCA	GACTGCGTACGAATTCAG
me13em15	TGGGGACAACCCGGTCA	GACTGCGTACGAATTCTG
me14em1	TGGGGACAACCCGGTGT	GACTGCGTACGAATTAAT
me14em3	TGGGGACAACCCGGTGT	GACTGCGTACGAATTGAC

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表 3 莱豆种质资源数量性状

Table 3. Quantifiable traits of P. lunatus

品种编号Variety code	第一对真叶First pair of true leaves		顶端小叶Top lobule				主蔓粗Main stem diamete/cm	鲜荚fresh pods				鲜豆百粒重hundred-grain weight of frsh bean/g	干荚Dry pods				干豆百粒重hundred-grain weight of dry bean/g
品种编号Variety code	真叶长True leaf length/cm	真叶宽True leaf width/cm	叶长Leaf length/cm	叶宽Leaf width/cm	叶柄长Petiole length/cm	叶柄粗Petiole thickness/mm	主蔓粗Main stem diamete/cm	荚长Pod length/cm	荚宽Pod width/cm	荚重Pod weight/g	荚粒数pod seednumber	鲜豆百粒重hundred-grain weight of frsh bean/g	荚长Pod length/cm	荚宽Pod length/cm	荚重Pod weight/g	荚粒数pod seed number	干豆百粒重hundred-grain weight of dry bean/g
1	9.39	9.10	11.18	7.70	9.10	2.33	1.23	10.74	2.79	12.66	2.61	312.21	10.78	2.50	4.92	2.40	155.08
2	7.75	6.43	10.82	7.02	9.47	2.22	1.39	10.14	2.75	13.45	2.62	349.17	11.15	2.32	5.55	2.60	163.73
3	8.48	8.08	10.72	6.22	10.32	2.00	1.30	10.69	2.56	12.81	2.53	313.80	15.69	2.24	5.08	2.50	155.24
4	8.79	7.31	12.11	6.87	11.27	2.04	1.47	11.44	2.92	19.17	3.00	374.30	11.33	2.41	7.56	2.50	175.80
5	8.74	8.18	10.37	6.58	9.98	2.00	1.63	10.14	2.63	14.42	2.50	356.62	9.78	2.49	5.86	2.60	167.58
6	8.57	8.83	10.38	6.12	8.95	1.84	1.26	10.77	2.72	11.49	2.35	311.38	11.68	2.37	5.62	2.90	150.83
7	8.78	9.19	10.02	5.67	8.92	1.83	1.33	10.44	2.69	10.69	2.45	330.77	11.61	2.51	5.62	3.00	136.77
8	7.21	8.07	12.27	6.92	11.21	1.97	1.51	11.88	2.66	15.06	2.88	373.22	11.63	2.38	6.80	3.00	179.93
9	10.48	9.54	10.44	6.32	9.59	2.51	1.34	11.50	2.55	12.50	2.80	320.50	10.29	2.07	4.43	2.50	136.68
10	10.17	9.20	11.61	7.28	9.93	1.99	1.35	10.42	2.94	11.19	2.40	333.05	10.10	2.38	4.05	2.40	129.67
11	9.41	8.53	12.15	6.67	10.11	1.99	1.69	10.57	2.70	11.38	2.55	333.33	11.19	2.39	5.56	2.80	160.96
12	8.77	9.03	11.62	6.47	11.36	1.97	1.89	11.18	2.76	12.24	2.63	364.39	11.33	2.60	6.36	3.00	170.63
13	8.01	7.30	10.68	6.36	10.20	1.94	1.31	11.06	2.58	13.11	2.80	307.26	10.50	2.26	5.24	2.50	165.52
14	8.40	7.77	11.75	6.78	10.32	1.88	1.66	11.34	3.00	14.17	2.42	363.45	11.12	2.42	5.19	2.50	157.92
15	9.43	8.01	12.45	7.09	11.12	1.93	1.37	12.56	2.82	17.17	2.90	335.03	11.63	2.28	5.37	2.45	161.52
16	5.28	8.26	11.31	6.25	9.23	1.90	1.63	11.20	2.66	14.09	2.60	351.20	10.07	2.24	7.29	2.40	136.75
17	10.23	7.04	6.50	5.67	6.25	1.48	0.92	7.25	1.47	4.71	2.85	104.61	7.53	1.47	2.61	2.85	55.19
18	9.79	7.58	6.94	6.08	9.37	1.69	1.03	7.23	1.44	4.67	3.05	99.61	7.39	1.45	2.66	2.80	57.20
19	9.04	7.16	6.78	5.54	6.33	1.40	1.01	7.09	1.66	4.32	2.70	93.97	7.30	1.65	2.29	2.75	54.67
20	9.47	7.99	9.24	6.79	8.89	1.71	1.02	7.56	1.93	6.47	3.30	128.35	6.81	1.55	2.26	2.70	57.59
21	8.93	8.62	10.88	6.35	12.61	2.28	1.77	12.06	2.74	17.62	2.80	417.11	11.73	2.49	5.93	2.60	167.46
22	8.98	8.38	11.19	7.10	12.54	2.15	1.85	13.10	2.77	19.62	3.09	411.34	12.02	2.33	6.72	3.00	169.70

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表 4 28对引物的扩增结果

Table 4. Amplicon of 28 primer pairs

引物组合Primer combination	总带数Total bands	差异带Difference band	基本带Basic bands	多态性Polymorphism/ %
ME2EM13	9	9	0	100.0
ME4EM4	4	2	2	50.0
ME5EM8	8	8	0	100.0
ME5EM13	9	8	1	88.89
ME6EM2	7	5	2	71.43
ME6EM3	10	10	0	100.0
ME7EM12	5	4	1	80.0
ME8EM2	6	3	3	50.0
ME10EM2	5	5	0	100.0
ME10EM3	3	2	1	66.67
ME10EM4	3	3	0	100.0
ME11EM4	6	5	1	83.33
ME11EM7	6	6	0	100.0
ME8EM11	5	4	1	80.0
ME12EM4	6	4	2	66.67
ME13EM1	8	5	3	62.5
ME13EM3	5	4	1	80.0
ME13EM9	4	4	0	100.0
ME13EM14	4	4	0	100.0
ME13EM15	4	1	3	25.0
ME14EM1	4	2	2	50.0
ME14EM3	6	4	2	66.67
ME4EM2	5	4	1	80.0
ME4EM11	5	3	2	60.0
ME5EM2	5	4	1	80.0
ME8EM4	7	6	1	85.71
ME2EM20	5	5	0	100.0
ME13EM5	4	2	2	50.0
合计Total	158	126	32
平均 Mean	5.64	4.5	1.14	77.75

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