脉冲电场对金边龙舌兰光合电子传递链和释放负离子能力影响

刘津; 翁海勇; 程致元; 吴仁烨

doi:10.19303/j.issn.1008-0384.2024.07.007

脉冲电场对金边龙舌兰光合电子传递链和释放负离子能力影响

doi: 10.19303/j.issn.1008-0384.2024.07.007

刘津^{1, 2,},
翁海勇³,
程致元¹,
吴仁烨^{1, 2, ,}

1.
福建农林大学农学院，福建　福州　350002
2.
农业农村部海峡两岸农业技术合作中心，福建　福州　350002
3.
福建农林大学机电工程学院，福建　福州　350002

基金项目: 福建省自然科学基金项目（2021J01077）；福建农林大学科技创新专项基金（CXZX2019030G、CXZX2019031）

详细信息

作者简介:
刘津（2000 —），男，硕士，主要从事植物负离子释放研究，E-mail：liujin20000606@163.com

通讯作者:
吴仁烨（1983 —），男，博士，副教授，主要从事植物负离子释放研究，E-mail：renye.wu@fafu.edu.cn

中图分类号: S563.8
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- 被引次数: 0
出版历程
- 收稿日期: 2024-04-18
- 修回日期: 2024-05-25
- 网络出版日期: 2024-10-25
- 刊出日期: 2024-07-01

Effects of Pulsed Electric Field on Photosynthetic Electron Transport and Negative Air Ion Releasing of Agave americana

LIU Jin^{1, 2
,},
WENG Haiyong³,
CHENG Zhiyuan¹,
WU Renye^{1, 2
, ,}

1.
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Ministry of Agriculture and Rural Affairs Cross-strait Agricultural Technology Cooperation Center, Fuzhou, Fujian　350002, China
3.
College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

摘要

摘要: 目的探明脉冲电场刺激对金边龙舌兰（ Agave americana var. Marginata）光合电子传递链和释放负离子能力的影响。方法采用0 kV（对照，CK）、5 kV（T1）和7 kV（T2）3个不同脉冲电场处理刺激金边龙舌兰，比较和分析不同刺激下金边龙舌兰的叶绿素含量、快速叶绿素荧光诱导动力曲线（Rapid Chlorophyll Fluorescence Induction Kinetics Curve， OJIP曲线）、叶绿素荧光参数和负离子释放浓度的变化情况。结果（1）脉冲电场技术刺激金边龙舌兰后，两处理组叶绿素含量分别增长6.30%、6.70%，两处理组与CK组之间存在显著差异；（2）两处理组OJIP曲线，偏转（I）和最高峰（P）值均高于原点（O）和拐点（J）值，I-P段的上升趋势较O-J段平缓。同时，J-band均大于0，T2处理组峰值高于T1处理组；（3）叶绿素荧光参数均呈波动的变化趋势，其中最大荧光强度（F_m）、单位面积捕获能量（TR_o/CS_o）等整体呈现上升的趋势，PSⅡ光合系统潜在活性（F_v/F_o）、PSⅡ受体侧电子传递体数（S_m）等整体呈现下降的趋势，最大光量子效率（F_v /F_m）、J点相对可变荧光强度（V_j）等整体变化不大。吸收光能为基础的性能指数（PI_abs）均出现减小的趋势，其中T1处理组减小18.60%，T2处理组减小14.1%。CK处理组综合性能参数（PI_total）大于T1处理组和T2处理组；（4）T1处理组，脉冲电场刺激4 h相较于脉冲刺激3 h时，NAI浓度增长87.60%。T2处理组，脉冲电场刺激4 h后，相较于脉冲刺激3 h时，NAI浓度增长62.09%，其中在脉冲1～4 h，两处理组之间、二者与CK组之间NAI释放浓度存在显著差异。结论脉冲电场技术对金边龙舌兰光合电子传递链产生影响，进而对其释放负离子能力等产生影响。
- 金边龙舌兰 /
- 脉冲电场 /
- 负离子 /
- OJIP曲线 /
- 叶绿素含量 /
- 叶绿素荧光参数
Abstract: Objective To investigate the effects of pulsed electric field on the photosynthetic electron transport and ability to release negative air ions (NAIs) of Agave americana were investigated. Method Chlorophyll content, rapid chlorophyll fluorescence kinetic functions (OJIP curve), chlorophyll fluorescence parameters, and NAI concentration of A. americana var. Marginata exposed to a pulsed electric field at 0 kV (CK), 5 kV (T1), or 7 kV (T2) were determined. Result (1) T1 and T2 significantly increased the chlorophyll content over CK by 6.30% and 6.70%, respectively. (2) The OJIP curves had higher deflection (I) and peak (P) than the origin (O) and inflection point (J), a flatter upward trend on I-P than on O-J, and a greater than 0 J-band with the peak of T2 higher than that of T1. (3) T1 and T2 also caused the chlorophyll fluorescence parameters to generally rise on the peak fluorescence intensity (F_m) and energy captured by unit reaction center (TR_o/CS_o), fall on the PSⅡ light, system potential activity (F_v/F_o), and number of electron transmitters on PSⅡ acceptor side (S_m), and maintain a stable maximum light quantum efficiency (F_v/F_m) as well as fluorescence at J-step (V_j). The performance index based on light energy absorption (PI_abs) decreased with an 18.60% reduction by T1 and 14.1% by T2. The comprehensive performance parameter (PI_total) under CK was greater than that under T1 or T2. (4) After a 4h treatment, the NAI concentration increased 87.60% under T1 and 62.09% under T2 from an hour previously. And, during that time, the concentrations differed significantly between T1 and T2, as well as in comparison with CK. Conclusion Exposure to a pulsed electric field significantly affected the photosynthetic electron transport and NAIs releasing of A. americana. The findings disclosed a means to identify potentially applicable germplasms and a physical treatment to enrich the unique biological function of the plant.
- Agave americana var. Marginata /
- pulsed electric field /
- negative air ions /
- OJIP curves /
- chlorophyll content /
- chlorophyll fluorescence parameters

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图 1 经脉冲电场刺激后金边龙舌兰叶片快速叶绿素荧光动力学曲线（OJIP）分析

a：5 kV （T1处理组）；b：7 kV（T2处理组）。图2、3同。

Figure 1. OJIP curves on leaves of A. americana treated by pulsed electric field

a: 5 kV (T1); b: 7 kV (T2). Same for Figs. 2 and 3.

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图 2 不同脉冲电场刺激金边龙舌兰叶片不同时间∆W_J（J-band）的差异分析

Figure 2. ∆W_J (J-band) of A. americana treated by pulse electric field for varied durations

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图 3 脉冲电场刺激金边龙舌兰叶片不同时间段叶绿素荧光参数动态变化

Figure 3. Dynamic changes on chlorophyll fluorescence parameters in leaves of A. americana treated by pulsed electric field for varied durations

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图 4 脉冲电场刺激后金边龙舌兰释放空气负离浓度的动态变化

Figure 4. Dynamic changes on NAI concentration after pulsed electric field treatments

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表 1 经脉冲电场刺激处理后金边龙舌兰叶片叶绿素含量变化

Table 1. Changes on chlorophyll content in leaves of A. americana treated by pulsed electric field

处理 Treatment	叶片SPAD值($ \overline{X}\pm\mathit{\mathit{\mathrm{\mathit{S D}}}} $) SPAD value($ \overline{X}\pm\mathrm{\mathit{S D}} $)
处理 Treatment	0 h	1 h	2 h	3 h	4 h
0 kV（CK）	71.92±0.79a	71.12±1.69b	70.05±1.58b	70.39±1.16b	72.94±0.81b
5 kV（T1）	72.54±0.86a	75.72±0.74a	74.46±2.65a	76.50±0.85a	77.11±0.69a
7 kV（T2）	72.44±0.98a	75.73±0.63a	75.22±2.30a	75.51±0.71a	77.28±1.81a
同列数据后不同小写字母表示同一时间不同处理组间差异显著（P＜0.05），表2、3同。 Different lowercase letters in the same column indicate the significant (P＜0.05) difference among treatments.Same for Table 2 and 3.

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表 2 脉冲电场刺激后金边龙舌兰叶绿素荧光参数的动态变化

Table 2. Dynamic changes on chlorophyll fluorescence parameters in leaves of A. americana treated by pulsed electric field ($\overline X \pm {{S D}}$)

项目 Item	处理 Treatment	叶绿素荧光参数值 ($ \overline X \pm {{S D}}$) Chlorophyll fluorescence parameter values ($\overline X \pm {{S D}}$)
项目 Item	处理 Treatment	0 h	1 h	2 h	3 h	4 h
	0 kV（CK）	5.25±0.14b	5.17±0.68a	5.41±0.23a	5.22±0.27a	5.39±0.15ab
Fv/Fo	5 kV（T1）	5.53±0.09a	5.36±0.26a	5.26±0.27a	5.14±0.28a	5.10±0.23b
	7 kV（T2）	5.42±0.30ab	5.42±0.29a	5.40±0.24a	5.41±0.19a	5.30±0.24a
	0 kV（CK）	27.38±5.78a	27.44±4.37a	28.78±4.82a	28.56±5.26a	28.46±4.91a
Sm	5 kV（T1）	29.07±5.24a	29.73±4.78a	29.73±0.01a	28.32±4.29a	27.72±4.17a
	7 kV（T2）	27.47±3.09a	28.00±3.25a	27.12±3.49a	27.96±4.90a	26.77±3.73a
	0 kV（CK）	0.52±0.02a	0.53±0.01a	0.52±0.02a	0.50±0.02b	0.51±0.02a
φEo	5 kV（T1）	0.52±0.01a	0.51±0.01a	0.51±0.01a	0.52±0.00a	0.50±0.01a
	7 kV（T2）	0.53±0.02a	0.52±0.02a	0.52±0.02a	0.52±0.02a	0.51±0.02a
	0 kV（CK）	9.05±1.51a	8.89±2.15a	9.24±1.65a	9.16±1.65a	9.47±1.50a
PIabs	5 kV（T1）	9.77±0.30a	9.22±0.64a	8.81±0.41a	8.42±0.28a	8.24±0.53a
	7 kV（T2）	10.75±1.4a	10.25±11a	10.09±1.27a	9.83±1.98a	9.42±1.51a
	0 kV（CK）	3.20±0.70a	2.94±0.97b	3.12±0.71a	2.95±0.68b	3.22±0.57a
PItotal	5 kV（T1）	3.25±1.31a	3.23±0.65a	3.13±0.58a	3.00±0.58a	2.87±1.81a
	7 kV（T2）	3.29±0.81a	3.19±0.72b	3.15±0.87a	3.10±0.94a	2.92±0.83a

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表 3 脉冲电场刺激后金边龙舌兰释放空气负离浓度的动态变化($ \overline X \pm {{S D}} $)

Table 3. Dynamic changes on NAI released by A. americana after pulse electric field treatments ($ \overline X \pm {{S D}} $)

不同处理组 Treatment group	负离子浓度变化 Negative ion concentration changes/(个·cm⁻³)
不同处理组 Treatment group	0 h	1 h	2 h	3 h	4 h
0 kV（CK）	521.36±17.86a	406.25±32.60b	364.45±12.35b	539.33±14.29b	400.78±10.25b
5 kV（T1）	526.17±19.67a	41986.22±5293.44a	126217.45±119339.75a	222001.80±256322.90a	416466.58±328669.54a
7 kV（T2）	592.93±21.17a	32412.12±25969.57ab	182844.66±93041.30a	131039.77±44299.84a	212401.10±91149.66ab

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