Biological Functions of Transcription Factor OmpR in <i>Serratia marcescens</i> FZSF02

JIA Xianbo; LIU Fangchen; ZHAO Ke; LIN Junjie; FANG Yu; CHEN Jichen

doi:10.19303/j.issn.1008-0384.2021.12.014

Volume 36 Issue 12

Dec. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(12): 1491-1498

JIA X B, LIU F C, ZHAO K, et al. Biological Functions of Transcription Factor OmpR in Serratia marcescens FZSF02 [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1491−1498 doi: 10.19303/j.issn.1008-0384.2021.12.014

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JIA X B, LIU F C, ZHAO K, et al. Biological Functions of Transcription Factor OmpR in Serratia marcescens FZSF02 [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1491−1498 doi: 10.19303/j.issn.1008-0384.2021.12.014

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Biological Functions of Transcription Factor OmpR in Serratia marcescens FZSF02

doi: 10.19303/j.issn.1008-0384.2021.12.014

1.
Institute of Soil and Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350001, China
3.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350001, China

Received Date: 2021-10-08
Rev Recd Date: 2021-11-12

Available Online: 2021-12-30

Publish Date: 2021-12-28

Abstract

Abstract

Objective Biological functions of the regulatory protein ompR in the two component EnvZ/OmpR system, including prodigiosin-producing ability and other biological characteristics, of Serratia marcescens FZSF02 were studied. Methods Homologous recombination was used to construct ompR-knockout S. marcescens FZSF02. Effect of OmpR on the prodigiosin-producing ability was examined by LB agar plate incubation and qPCR. Methods of crystal violet staining, agar plate incubation, and others were applied to determine the biofilm-forming, mobility, and stress adaptation abilities of the transcription factor protein under various environmental stresses. Results OmpR was a protein with high conserved amino acid sequences. An ompR- deleted strain, FZSF02 ∆ompR, was successfully obtained by homologous recombination and confirmed by PCR. As a result, FZSF02 ∆ompR lost prodigiosin-producing ability that possessed by the wild strain. The transcriptional levels of pigA, pigF, and pigN of the prodigiosin biosynthesis gene cluster in FZSF02 ∆ompR were respectively 3.8%, 2.0% and 2.1% of the wild type strain. The biofilm formation of FZSF02 ∆ompR declined 37.5% (at 37 ℃) and 15.1% (at 27 ℃) from its wild counterpart. On the other hand, OmpR exhibited no significant effect on the growth, mobility, or response to the environmental stress. Conclusion OmpR was a newly reported gene that specifically regulated the prodigiosin biosynthesis in S. marcescens. It also significantly affected the biofilm formation but not on the growth, mobility, or stress response.
- Serratia marcescens,
- OmpR,
- prodigiosin,
- biological characteristics

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References(29)

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