Identification and Characterization of Phosphate-solubilizing Endophytes in <i>Coix lacryma</i>-jobi L.

PU Fengya; GU Shujie; HE Yonghong; CHEN Songlin; YANG Zhiqing

doi:10.19303/j.issn.1008-0384.2022.007.016

Volume 37 Issue 7

Jul. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(7): 946-953

PU F Y, GU S J, HE Y H, et al. Identification and Characterization of Phosphate-solubilizing Endophytes in Coix lacryma-jobi L. [J]. Fujian Journal of Agricultural Sciences，2022，37（7）：946−953 doi: 10.19303/j.issn.1008-0384.2022.007.016

Citation:

PU F Y, GU S J, HE Y H, et al. Identification and Characterization of Phosphate-solubilizing Endophytes in Coix lacryma-jobi L. [J]. Fujian Journal of Agricultural Sciences，2022，37（7）：946−953 doi: 10.19303/j.issn.1008-0384.2022.007.016

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Identification and Characterization of Phosphate-solubilizing Endophytes in Coix lacryma-jobi L.

doi: 10.19303/j.issn.1008-0384.2022.007.016

PU Fengya^1
,,
GU Shujie¹,
HE Yonghong²,
CHEN Songlin¹,
YANG Zhiqing^{1, 3, 4
,
,}

1.
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan　650201, China
2.
College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan　650201, China
3.
Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan　650201, China
4.
National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming, Yunnan　650201, China

Received Date: 2022-03-01
Rev Recd Date: 2022-05-09

Available Online: 2022-08-07

Publish Date: 2022-07-28

Abstract

Abstract

Objective Screening bacteria with phosphate-solubilizing ability in Coix were identified and provide excellent strain resource for bio-fertilization agent. Methods Endophytic bacteria were isolated and purified from specimens the roots, stems, and leaves of 25-d-old seedlings of Coix lacryma-jobi L. Abilities of the isolates in dissolving organophosphate and inorganic phosphate as well as in secreting phosphatase were determined. In a pot experiment, effects of two selected isolates on the growth of Coix seedlings were examined. Results Eight strains of bacteria capable of dissolving organophosphate and 5 of degrading inorganic phosphate were isolated. In the 5-d culture supernatants, the highest concentration of phosphorous converted from organophosphate at 37.20 mg·L⁻¹ was reached by R24, and that from inorganic phosphate at 62.93 mg·L⁻¹ by L21. The enhancing effects on plant height, stem girth, and tillering number of the potted Coix seedlings by each and mixture in equal proportion of the two supernatants ranked in the order of L21＞R24＞mixed bacterial liquid[ V （R24）∶ V （L21）=1∶1]. The 16S rDNA sequence phylogenetic analysis identified R24 as Bacillus pumilus and L21 as B. velezensis. Conclusion The phosphobacteria L21 and R24 displayed a significant effect in promoting the growth of Coix seedlings.
- Coix,
- endophytes,
- phosphobacteria,
- phosphate-solubilizing ability,
- identification

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References(22)

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