A Preliminary Study on Genetic and Serological Clustering of Pathogenic <i>Pseudomonas plecoglossicida</i> in <i>Larimichthys crocea</i>

XU Binfu; CHEN Xiujin; CHI Hongshu; XU Mengting; CHEN Jia; LIN Nengfeng; GONG Hui

doi:10.19303/j.issn.1008-0384.2022.009.001

Volume 37 Issue 9

Sep. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(9): 1109-1116

XU B F, CHEN X J, CHI H S, et al. A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1109−1116 doi: 10.19303/j.issn.1008-0384.2022.009.001

Citation:

XU B F, CHEN X J, CHI H S, et al. A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1109−1116 doi: 10.19303/j.issn.1008-0384.2022.009.001

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A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea

doi: 10.19303/j.issn.1008-0384.2022.009.001

XU Binfu^1
,,
CHEN Xiujin²,
CHI Hongshu¹,
XU Mengting¹,
CHEN Jia³,
LIN Nengfeng^{1
,
,},
GONG Hui^{1
,
,}

1.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Fujian Center for Disease Control and Prevention, Fuzhou, Fujian　350002, China
3.
State Key Laboratory of Large Yellow Croaker Breeding, Ningde, Fujian　352103, China

Received Date: 2022-06-02
Accepted Date: 2022-06-02
Rev Recd Date: 2022-09-02

Available Online: 2022-10-05

Publish Date: 2022-09-30

Abstract

Abstract

Objective Clustering of strains of Pseudomonas plecoglossicida, a major pathogen that causes the serious bacterial visceral white spot disease on Larimichthys crocea in winter and spring, was studied for disease control and epidemic prevention. Method The 16S rDNA and gyrB of 7 suspected P. plecoglossicida strains collected from different temporal and spatial sources were cloned and sequenced with evolutionary trees constructed. O and H antigen serums of these strains were prepared for agglutination tests. Result The 7 pathogens could be clustered into a same branch based on their sequences. Their O and H antigen sera agglutinated with those of the standard P. plecoglossicida reference without significant differences on titers, but not with that of P. fluoresenes, Vibrio alginolyticus, or V. harveyi. Conclusion It appeared that the 7 collected strains of P. plecoglossicida were genetically closely related and might have a common origin.
- Larimichthys crocea,
- Pseudomonas plecoglossicida,
- 16S rDNA,
- gyrB,
- bacterial and serum agglutination,
- cluster analysis

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References(19)

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