Effects of Exogenous Melatonin on Cold Resistance of Green Pepper Seedlings

LIAN Dongmei; LAI Zhengfeng; LI Zhou; LIN Bizhen; YAO Yunfa; ZHANG Shaoping; HONG Jianji

doi:10.19303/j.issn.1008-0384.2022.010.006

Volume 37 Issue 10

Oct. 2022

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(10): 1281-1287

LIAN D M, LAI Z F, LI Z, et al. Effects of Exogenous Melatonin on Cold Resistance of Green Pepper Seedlings [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1281−1287 doi: 10.19303/j.issn.1008-0384.2022.010.006

Citation:

LIAN D M, LAI Z F, LI Z, et al. Effects of Exogenous Melatonin on Cold Resistance of Green Pepper Seedlings [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1281−1287 doi: 10.19303/j.issn.1008-0384.2022.010.006

Citation:

PDF( 743 KB)

Effects of Exogenous Melatonin on Cold Resistance of Green Pepper Seedlings

doi: 10.19303/j.issn.1008-0384.2022.010.006

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian　363005, China

Received Date: 2022-08-05
Rev Recd Date: 2022-09-03

Available Online: 2022-10-21

Publish Date: 2022-10-30

Abstract

Abstract

Objective Physiological mechanism and exogenous melatonin (MT) application to improve cold tolerance of green pepper seedlings were investigated. Method Seedlings of green pepper cultivar, Britney, were planted in matrix pots and sprayed with a MT solution of 0 (CK), 25, 50, 75, or 100 µmol·L⁻¹. Effects on the growth, antioxidant enzyme activity, osmotic regulatory substances, and photosynthetic characteristics of the seedlings under chilling at 4 ℃ were determined. Result Comparing to CK, spraying MT increased the contents of catalase (CAT), superoxide dismutase (SOD), and proline (Pro), as well as the net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s), maximum quantum yield (F_v/F_m), maximum photosynthetic potential (F_v/F_o), electron transport activity (F_m/F_o), leaf SPAD, while decreased malondialdehyde (MDA), relative electrical conductivity, and intercellular CO₂ concentration in leaf. At 50 µmol·L⁻¹ level, the MT spray showed the greatest effect in alleviating cold injury on the seedlings. The treatment also allowed the underground dry weight, CAT, SOD, MDA, relative conductivity, P_n, C_i, leaf SPAD, F_v/F_m, F_v/F_o, and F_m/F_oto reach significant heights and Pro, T_r, and G_s at extremely significant levels. Conclusion The exogenous MT spray mitigated injury by chilling temperature and improved cold tolerance of the green pepper seedlings through mechanisms such as a heightened antioxidant enzyme activity and photosynthetic rate as well as suppressed membrane lipid peroxidation and regulated osmotic substances. The optimum MT application on the green peeper plants was determined to be at 50 µmol·L⁻¹.
- Green pepper,
- seedlings,
- melatonin,
- cold stress,
- physiological mechanism

FullText(HTML)

References(35)

References

[1]	李天来. 设施蔬菜栽培学[M]. 北京: 中国农业出版社, 2011.
[2]	CARUSO G, STOLERU V V, MUNTEANU N C, et al. Quality performances of sweet pepper under farming management [J]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2018, 47(2): 458−464. doi: 10.15835/nbha47111351
[3]	FRATIANNI F, D'ACIERNO A, COZZOLINO A, et al. Biochemical characterization of traditional varieties of sweet pepper (Capsicum annuum L. ) of the Campania region, southern Italy [J]. Antioxidants (Basel, Switzerland), 2020, 9(6): 556.
[4]	杨若鹏, 赵欣, 李许华, 等. 珍珠岩引发对盐胁迫下青椒种子萌发和幼苗生长的影响 [J]. 云南农业大学学报(自然科学), 2018, 33(3):468−473. YANG R P, ZHAO X, LI X H, et al. Effects of perlite priming on the seed germination and seedling growth of pepper under salt stress [J]. Journal of Yunnan Agricultural University (Natural Science), 2018, 33(3): 468−473.(in Chinese)
[5]	姚运法, 林一心, 赖正锋, 等. 诏安县设施青椒冻害调查及预防措施 [J]. 福建热作科技, 2021, 46(4):45−46. YAO Y F, LIN Y X, LAI Z F, et al. Investigation about freezing injury and preventative measures of greenpepper in greenhouse at zhao'an County [J]. Fujian Science & Technology of Tropical Crops, 2021, 46(4): 45−46.(in Chinese)
[6]	吴杨洋, 郝莹超, 苏振华, 等. 甜椒耐寒种质耐低温IL系的筛选及低温胁迫下生理应答差异 [J]. 分子植物育种, 2019, 17(4):1249−1256. WU Y Y, HAO Y C, SU Z H, et al. Screening of low temperature introgression lines and the differences in physiological response under cold stress of sweet pepper hardy germplasm [J]. Molecular Plant Breeding, 2019, 17(4): 1249−1256.(in Chinese)
[7]	张天翔, 林宗铿, 曹明华, 等. 不同甜椒品种抗寒性研究初报 [J]. 福建农业学报, 2014, 29(2):160−164. ZHANG T X, LIN Z K, CAO M H, et al. Preliminary study on cold resistance of different sweet pepper varieties [J]. Fujian Journal of Agricultural Sciences, 2014, 29(2): 160−164.(in Chinese)
[8]	罗立津, 徐福乐, 翁华钦, 等. 脱落酸对甜椒幼苗抗寒性的诱导效应及其机理研究 [J]. 西北植物学报, 2011, 31(1):94−100. LUO L J, XU F L, WENG H Q, et al. Inducing effects and its biological mechanisms of ABA on the chilling resistance of sweet pepper seedlings [J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(1): 94−100.(in Chinese)
[9]	徐福乐, 罗立津, 高灿红. 植物生长调节剂对甜椒的抗寒性诱导效应研究 [J]. 农药学学报, 2011, 13(1):33−39. XU F L, LUO L J, GAO C H. Inducing effects of plant growth regulators on the chilling resistance of sweet pepper(Capsicum annuum) [J]. Chinese Journal of Pesticide Science, 2011, 13(1): 33−39.(in Chinese)
[10]	WU X X, DING H D, CHEN J L, et al. Exogenous spray application of 24-epibrassinolide induced changes in photosynthesis and anti-oxidant defences against chilling stress in eggplant (Solanum melongena L. ) seedlings [J]. The Journal of Horticultural Science and Biotechnology, 2015, 90(2): 217−225. doi: 10.1080/14620316.2015.11513175
[11]	巩彪, 史庆华. 园艺作物褪黑素的研究进展 [J]. 中国农业科学, 2017, 50(12):2326−2337. GONG B, SHI Q H. Review of melatonin in horticultural crops [J]. Scientia Agricultura Sinica, 2017, 50(12): 2326−2337.(in Chinese)
[12]	MANCHESTER L C, COTO-MONTES A, BOGA J A, et al. Melatonin: an ancient molecule that makes oxygen metabolically tolerable [J]. Journal of Pineal Research, 2015, 59(4): 403−419. doi: 10.1111/jpi.12267
[13]	刘德帅, 姚磊, 徐伟荣, 等. 褪黑素参与植物抗逆功能研究进展 [J]. 植物学报, 2022, 57(1):111−126. LIU D S, YAO L, XU W R, et al. Research progress of melatonin in plant stress resistance [J]. Chinese Bulletin of Botany, 2022, 57(1): 111−126.(in Chinese)
[14]	吴雪霞, 朱宗文, 张爱冬, 等. 外源褪黑素对低温胁迫下茄子幼苗生长及其光合作用和抗氧化系统的影响 [J]. 西北植物学报, 2017, 37(12):2427−2434. WU X X, ZHU Z W, ZHANG A D, et al. Effects of exogenous melatonin on the growth, photosynthesis and antioxidant system in eggplant (Solanum melongena L. ) seedlings under low temperature stress [J]. Acta Botanica Boreali-Occidentalia Sinica, 2017, 37(12): 2427−2434.(in Chinese)
[15]	李贺, 姜欣悦, 陈忠诚, 等. 外源褪黑素对低温胁迫下大豆V₁期幼苗光合荧光及抗氧化系统的影响 [J]. 中国油料作物学报, 2020, 42(4):640−648. LI H, JIANG X Y, CHEN Z C, et al. Effects of exogenous melatonin on photosynthetic fluorescence and antioxidant system of soybean V₁ seedlings under low temperature [J]. Chinese Journal of Oil Crop Sciences, 2020, 42(4): 640−648.(in Chinese)
[16]	朱春权, 魏倩倩, 项兴佳, 等. 褪黑素和茉莉酸甲酯基质育秧对水稻耐低温胁迫的调控作用 [J]. 作物学报, 2022, 48(8):2016−2027. ZHU C Q, WEI Q Q, XIANG X J, et al. Regulation effects of seedling raising by melatonin and methyl jasmonate substrate on low temperature stress tolerance in rice [J]. Acta Agronomica Sinica, 2022, 48(8): 2016−2027.(in Chinese)
[17]	王彬, 田正凤, 应彬彬, 等. 高温胁迫对樟树光合性能的影响 [J]. 浙江农林大学学报, 2019, 36(1):47−53. WANG B, TIAN Z F, YING B B, et al. Photosynthetic abilities in Cinnamomum camphora with high temperature stress [J]. Journal of Zhejiang A & F University, 2019, 36(1): 47−53.(in Chinese)
[18]	许耀照, 张芬琴, 陈修斌, 等. 低温胁迫对彩椒幼苗生长指标及光合特性的影响 [J]. 山西农业大学学报(自然科学版), 2019, 39(1):68−72. XU Y Z, ZHANG F Q, CHEN X B, et al. Effects of low temperature stress on growth index and photosynthetic characteristics of pepper seedlings [J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2019, 39(1): 68−72.(in Chinese)
[19]	刘凯歌, 龚繁荣, 宋云鹏, 等. 低温弱光对甜椒幼苗生长和生理生化指标的影响及其与品种耐性的关系 [J]. 北方园艺, 2020(3):8−14. LIU K G, GONG F R, SONG Y P, et al. Effects of low temperature and weak light on the growth and physiological and biochemical indexes of sweet pepper seedlings and their relationship to varieties tolerance [J]. Northern Horticulture, 2020(3): 8−14.(in Chinese)
[20]	和秋兰, 张航, 王正维, 等. 外源褪黑素对低温胁迫下马铃薯幼苗抗氧化系统的影响 [J]. 华北农学报, 2022, 37(1):103−111. HE Q L, ZHANG H, WANG Z W, et al. Effects of exogenous melatonin on antioxidant system of potato seedlings under low temperature stress [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 103−111.(in Chinese)
[21]	SHAHEEN R, HOOD-NOWOTNY R C. Effect of drought and salinity on carbon isotope discrimination in wheat cultivars [J]. Plant Science, 2005, 168(4): 901−909. doi: 10.1016/j.plantsci.2004.11.003
[22]	CHEN Q, QI W B, REITER R J, et al. Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea [J]. Journal of Plant Physiology, 2009, 166(3): 324−328. doi: 10.1016/j.jplph.2008.06.002
[23]	NOORI M, AZAR A M, SAIDI M, et al. Evaluation of water deficiency impacts on antioxidant enzymes activity and lipid peroxidation in some tomato (Solanum lycopersicum L. ) Lines [J]. Indian Journal of Agricultural Research, 2018, 52(3): 228−235.
[24]	王小华, 庄南生. 脯氨酸与植物抗寒性的研究进展 [J]. 中国农学通报, 2008, 24(11):398−402. WANG X H, ZHUANG N S. Advances in research on proline and cold resistance of plant [J]. Chinese Agricultural Science Bulletin, 2008, 24(11): 398−402.(in Chinese)
[25]	TANG L, KWON S Y, KIM S H, et al. Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature [J]. Plant Cell Reports, 2006, 25(12): 1380−1386. doi: 10.1007/s00299-006-0199-1
[26]	施海涛. 植物逆境生理学实验指导[M]. 北京: 科学出版社, 2016.
[27]	ZHANG N, ZHAO B, ZHANG H J, et al. Melatonin promotes water-stress tolerance, lateral root formation, and seed germination in cucumber (Cucumis sativus L. ) [J]. Journal of Pineal Research, 2013, 54(1): 15−23. doi: 10.1111/j.1600-079X.2012.01015.x
[28]	BAWA G, FENG L Y, SHI J Y, et al. Evidence that melatonin promotes soybean seedlings growth from low-temperature stress by mediating plant mineral elements and genes involved in the antioxidant pathway [J]. Functional Plant Biology:FPB, 2020, 47(9): 815−824. doi: 10.1071/FP19358
[29]	李涛. 群体密度对高等植物光合功能的影响及调控机制[D]. 北京: 北京林业大学, 2015. LI T. Effects of planting density on photosynthetic function and regulative mechanisms in higher plants[D]. Beijing: Beijing Forestry University, 2015. (in Chinese)
[30]	荆培培, 任红茹, 杨洪建, 等. 盐胁迫对2个不同盐敏感性水稻品种(系)叶片光合特性与产量的影响 [J]. 作物杂志, 2020(1):67−75. JING P P, REN H R, YANG H J, et al. Effects of saline stress on leaf photosynthesis characteristics and grain yield of two rice cultivars(lines) [J]. Crops, 2020(1): 67−75.(in Chinese)
[31]	SU L Y, DAI Z W, LI S H, et al. A novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence [J]. BMC Plant Biology, 2015, 15: 82. doi: 10.1186/s12870-015-0459-8
[32]	MARTA B, SZAFRAŃSKA K, POSMYK M M. Exogenous melatonin improves antioxidant defense in cucumber seeds (Cucumis sativus L.) germinated under chilling stress [J]. Frontiers in Plant Science, 2016, 7: 575.
[33]	ZHANG M C, HE S Y, ZHAN Y C, et al. Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean [J]. PLoS One, 2019, 14(12): e0226542. doi: 10.1371/journal.pone.0226542
[34]	张立业, 邱佳奇, 王晓香, 等. 外源褪黑素对低温胁迫下红树莓叶片生理特性的影响 [J]. 果树学报, 2022, 39(3):416−425. ZHANG L Y, QIU J Q, WANG X X, et al. Effects of exogenous melatonin on physiological characteristics of leaves under low-temperature stress in red raspberry [J]. Journal of Fruit Science, 2022, 39(3): 416−425.(in Chinese)
[35]	尉欣荣, 张智伟, 周雨, 等. 褪黑素对低温和干旱胁迫下多年生黑麦草幼苗生长和抗氧化系统的调节作用 [J]. 草地学报, 2020, 28(5):1337−1345. YU X R, ZHANG Z W, ZHOU Y, et al. Effects of melatonin on growth and antioxidant system of perennial ryegrass seedlings under cold and drought stresses [J]. Acta Agrestia Sinica, 2020, 28(5): 1337−1345.(in Chinese)