Role of Tail Fiber Protein in Attaching <i>Riemerella anatipestifer </i>to Phage

LIU Na; HUANG Yu; CHEN Hongmei; JIANG Nansong; LIU Rongchang; FU Guanghua; FU Qiuling; LIANG Qizhang; WAN Chunhe; LI Ang; CHENG Longfei

doi:10.19303/j.issn.1008-0384.2024.01.001

Volume 39 Issue 1

Jan. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(1): 1-6

LIU N, HUANG Y, CHEN H M, et al. Role of Tail Fiber Protein in Attaching Riemerella anatipestifer to Phage [J]. Fujian Journal of Agricultural Sciences，2024，39（1）：1−6 doi: 10.19303/j.issn.1008-0384.2024.01.001

Citation:

LIU N, HUANG Y, CHEN H M, et al. Role of Tail Fiber Protein in Attaching Riemerella anatipestifer to Phage [J]. Fujian Journal of Agricultural Sciences，2024，39（1）：1−6 doi: 10.19303/j.issn.1008-0384.2024.01.001

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Role of Tail Fiber Protein in Attaching Riemerella anatipestifer to Phage

doi: 10.19303/j.issn.1008-0384.2024.01.001

LIU Na^1
,,
HUANG Yu^{2, 3, 4},
CHEN Hongmei^{2, 3, 4},
JIANG Nansong^{2, 3, 4},
LIU Rongchang^{2, 3, 4},
FU Guanghua^{2, 3, 4},
FU Qiuling^{2, 3, 4},
LIANG Qizhang^{2, 3, 4},
WAN Chunhe^{2, 3, 4},
LI Ang^{1
,
,},
CHENG Longfei^{2, 3, 4
,
,}

1.
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
3.
Fujian Industry Technology Innovation Research Academy of Livestock and Poultry Diseases Prevention and Control, Fuzhou, Fujian　350013, China
4.
Fujian Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian　350013, China

Received Date: 2023-09-17
Rev Recd Date: 2023-11-14

Available Online: 2024-01-25

Publish Date: 2024-01-28

Abstract

Abstract

Objective Role of tail fiber protein (TFP) plays in adhering Riemerella anatipestifer by CRP2 phage was investigated. Method The putative TFP gene of ORF70 was cloned. The recombinant expression vector pET28a(+)-TFP was constructed and induced to express in E. coli BL21(DE3). The recombinant protein was purified by Ni-NTA affinity chromatography. ELISA was used to determine the binding effect of the recombinant protein with the outer membrane protein (OMP) of host bacteria. A competitive adsorption test was performed to determine the adhering effect of the CRP2 protein. Result The recombinant plasmids of pET28a(+)-TFP was successfully constructed and expressed in E. coli. The purified recombinant protein rTFP could bind to the OMP of or suppress the CRP2 adsorption to the host bacteria. Conclusion The TFP encoded by ORF70, one of the receptor binding proteins of CRP2 phage, was confirmed to adhere to the host bacteria OMP facilitating the subsequent bactericidal effect.
- Riemerella anatipestifer,
- phage,
- tail fiber protein,
- adsorption

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References(21)

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