Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian

WANG Pan; YU Wentao; LV Shuiyuan; ZHU Yanyu; LAN Zhenliang; LI Qingying; LI Guolai; GUI Wenjing; YE Naixing

doi:10.19303/j.issn.1008-0384.2024.01.009

Volume 39 Issue 1

Jan. 2024

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(1): 66-74

WANG P, YU W T, LV S Y, et al. Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian [J]. Fujian Journal of Agricultural Sciences，2024，39（1）：66−74 doi: 10.19303/j.issn.1008-0384.2024.01.009

Citation:

WANG P, YU W T, LV S Y, et al. Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian [J]. Fujian Journal of Agricultural Sciences，2024，39（1）：66−74 doi: 10.19303/j.issn.1008-0384.2024.01.009

Citation:

PDF( 2318 KB)

Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian

doi: 10.19303/j.issn.1008-0384.2024.01.009

1.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Technology Centre of Fuzhou Customs/Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Fuzhou, Fujian　350001, China
3.
Fujian Lufeng Tea Industry Co., Ltd., Yongtai, Fujian　350700, China
4.
Zishan Tianming Tea Professional Cooperative in Yongtai , Yongtai , Fujian　350704, China

Received Date: 2023-09-08
Rev Recd Date: 2023-11-14

Available Online: 2024-01-06

Publish Date: 2024-01-28

Abstract

Abstract

Objective Genetic diversity and population structure of tea germplasms in Yongtai County were analyzed for resource utilization. Method The EST-SNP molecular marker technology was applied to analyze the genetic relationships and diversity of 77 tea germplasms collected from Yongtai and surrounding areas. Result Forty-eight SNP loci for identifying the germplasms were selected. The average polymorphism information index of the loci was 0.407 with an average observed heterozygosity of 0.303 and an expected one of 0.271, as well as the average fixed index of −0.092 and the frequency of secondary alleles of 0.268. The principal coordinate, cluster, and structure population structure analyses revealed that the interactions within a same population of the tea plants were stronger than among different populations at Yongtai, and that the tea germplasms found in Wutong significantly differed from those in Fukou, Danyun, and other places with significant genetic distance but close to those from Youxi and Datian. Using the 48 selected high-quality SNP loci, a library of the DNA fingerprints on 54 germplasms from Yongtai was established to provide a means for accurate germplasm identification. Conclusion Yongtai County was rich in the resource of varieties of tea plants, which could be classified geographically into those from Wutong and those from Fukou, Danyun and other localities in the county. The cultivars from Wutong were more closely related to those found in Datian and Youxi, whereas those from Fukou, Danyun, etc. more to those existed in the north part of the county.
- Yongtai,
- Camellia sinensis,
- germplasms,
- genetic diversity,
- relationship,
- SNP

FullText(HTML)

References(33)

References

[1]	张文驹, 戎俊, 韦朝领, 等. 栽培茶树的驯化起源与传播 [J]. 生物多样性, 2018, 26(4):357−372. doi: 10.17520/biods.2018006 ZHANG W J, RONG J, WEI C L, et al. Domestication origin and spread of cultivated tea plants [J]. Biodiversity Science, 2018, 26(4): 357−372.(in Chinese) doi: 10.17520/biods.2018006
[2]	阚能才. 茶树起源与川渝野生茶树分布研究 [J]. 西南农业学报, 2013, 26(1):382−385. doi: 10.3969/j.issn.1001-4829.2013.01.080 KAN N C. Study on geographical distribution of wild tea trees in Sichuan and Chongqing, and origin of tea tree [J]. Southwest China Journal of Agricultural Sciences, 2013, 26(1): 382−385.(in Chinese) doi: 10.3969/j.issn.1001-4829.2013.01.080
[3]	陈橼. 茶业通史[M]. 2版. 北京: 中国农业出版社, 2008.
[4]	LIN Y, YU W T, ZHOU L, et al. Genetic diversity of oolong tea (Camellia sinensis) germplasms based on the nanofluidic array of single-nucleotide polymorphism (SNP) markers [J]. Tree Genetics & Genomes, 2019, 16(1): 3.
[5]	许长同, 郭玉琼, 张延辉, 等. 永泰古茶树研究与开发利用初报 [J]. 东南园艺, 2018, 6(4):7−10. doi: 10.3969/j.issn.2095-5774.2018.04.003 XU C T, GUO Y Q, ZHANG Y H, et al. A preliminary report on research, development and utilization of ancient tea tree in Yongtai County [J]. Southeast Horticulture, 2018, 6(4): 7−10.(in Chinese) doi: 10.3969/j.issn.2095-5774.2018.04.003
[6]	吴觉农, 吕允福, 张承春. 我国西南地区是世界茶树的原产地 [J]. 茶叶, 1979, 5(1):5−11. WU J N, LYU Y F, ZHANG C C. Southwest China is the origin of tea trees in the world [J]. Journal of Tea, 1979, 5(1): 5−11.(in Chinese)
[7]	叶伟森. 茶树种质资源的多样性及茶树景观分析 [J]. 分子植物育种, 2023, 21(15):5154−5159. YE W S. Diversity of tea germplasm resources and analysis of tea tree landscapes [J]. Molecular Plant Breeding, 2023, 21(15): 5154−5159.(in Chinese)
[8]	何环珠, 林文雄, 闵庆文, 等. 闽南古茶树资源价值与保护策略探讨 [J]. 生态与农村环境学报, 2022, 38(12):1508−1513. HE H Z, LIN W X, MIN Q W, et al. Discussion on the conservation and utilization of ancient tea tree resources in southern Fujian [J]. Journal of Ecology and Rural Environment, 2022, 38(12): 1508−1513.(in Chinese)
[9]	SAPKOTA D, ZHANG D P, PARK S, et al. Genotyping of jujube (Ziziphus spp. ) germplasm in new Mexico and southwestern texas [J]. Plants, 2023, 12(13): 2405. doi: 10.3390/plants12132405
[10]	LIU C G, YU W T, CAI C P, et al. Genetic diversity of tea plant (Camellia sinensis (L. ) Kuntze) germplasm resources in Wuyi Mountain of China based on single nucleotide polymorphism (SNP) markers [J]. Horticulturae, 2022, 8(10): 932. doi: 10.3390/horticulturae8100932
[11]	王小萍, 刘飞, 李明红, 等. 基于GBS测序的古蔺野生茶树遗传多样性与群体遗传结构分析 [J]. 西南农业学报, 2023, 36(6):1141−1149. WANG X P, LIU F, LI M H, et al. Genetic diversity and structure analysis of Gulin wild tea resources based on GBS technology [J]. Southwest China Journal of Agricultural Sciences, 2023, 36(6): 1141−1149.(in Chinese)
[12]	樊晓静, 于文涛, 王泽涵, 等. 基于EST-SNP的福鼎大白茶及其衍生品种的遗传关系分析 [J/OL]. 分子植物育种, 2022-01-13, http://kns.cnki.net/kcms/detail/46.1068.S.20220112.1947.014.html. FAN X J, YU W T, WANG Z H, et al. Genetic analysis of camellia sinensis ‘Fuding Dabaicha’ and Its derived cultivars using EST-SNP markers [J/OL]. Molecular Plant Breeding, 2022-01-13, http://kns.cnki.net/kcms/detail/46.1068.S.20220112.1947.014.html.
[13]	樊晓静, 于文涛, 蔡春平, 等. 利用SNP标记构建茶树品种资源分子身份证 [J]. 中国农业科学, 2021, 54(8):1751−1772. doi: 10.3864/j.issn.0578-1752.2021.08.014 FAN X J, YU W T, CAI C P, et al. Construction of molecular ID for tea cultivars by using of single-nucleotide polymorphism(SNP) markers [J]. Scientia Agricultura Sinica, 2021, 54(8): 1751−1772.(in Chinese) doi: 10.3864/j.issn.0578-1752.2021.08.014
[14]	王泽涵, 于文涛, 樊晓静, 等. 利用SNP标记构建漳州南部茶树种质资源的分子身份证 [J]. 江苏农业科学, 2022, 50(18):284−289. WANG Z H, YU W T, FAN X J, et al. Molecular ID of tea plant germplasm resources in Southern Zhangzhou by SNP markers [J]. Jiangsu Agricultural Sciences, 2022, 50(18): 284−289.(in Chinese)
[15]	朱晨, 田采云, 张舒婷, 等. 福建省53份茶树种质资源SSR-PCR反应体系的优化 [J]. 安徽农业科学, 2018, 46(28):88−91. doi: 10.3969/j.issn.0517-6611.2018.28.027 ZHU C, TIAN C Y, ZHANG S T, et al. Optimization of SSR-PCR reaction system for 53 tea (Camellia sinensis) germplasm resources in Fujian Province [J]. Journal of Anhui Agricultural Sciences, 2018, 46(28): 88−91.(in Chinese) doi: 10.3969/j.issn.0517-6611.2018.28.027
[16]	陈潇敏, 章进汕, 金珊, 等. 福建大田茶树品种资源生化成分特征分析与评价 [J]. 南方农业学报, 2022, 53(2):381−390. doi: 10.3969/j.issn.2095-1191.2022.02.011 CHEN X M, ZHANG J S, JIN S, et al. Analysis of biochemical components of tea variety resources in Datian, Fujian [J]. Journal of Southern Agriculture, 2022, 53(2): 381−390.(in Chinese) doi: 10.3969/j.issn.2095-1191.2022.02.011
[17]	王泽涵, 于文涛, 方德音, 等. 基于EST-SNP的福建云霄茶树种质资源遗传多样性分析 [J]. 福建农业学报, 2021, 36(12):1431−1438. WANG Z H, YU W T, FANG D Y, et al. EST-SNP marker-based genetic analysis on tea germplasms of Yunxiao in Fujian [J]. Fujian Journal of Agricultural Sciences, 2021, 36(12): 1431−1438.(in Chinese)
[18]	FANG W P, MEINHARDT L W, TAN H W, et al. Varietal identification of tea (Camellia sinensis) using nanofluidic array of single nucleotide polymorphism (SNP) markers [J]. Horticulture Research, 2014, 1: 14035. doi: 10.1038/hortres.2014.35
[19]	LIN Y, YU W T, CAI C P, et al. Rapid varietal authentication of oolong tea products by microfluidic-based SNP genotyping[J]. Food Research International, 2022, 162(Pt A): 111970.
[20]	陈亮, 杨亚军, 虞富莲. 中国茶树种质资源研究的主要进展和展望 [J]. 植物遗传资源学报, 2004, 5(4):389−392. CHEN L, YANG Y J, YU F L. Tea germplasm research in China: Recent progresses and prospects [J]. Journal of Plant Genetic Resources, 2004, 5(4): 389−392.(in Chinese)
[21]	张序, 张秀姣, 马永鹏, 等. 基于GBS简化基因组技术的宽杯杜鹃遗传多样性分析 [J]. 植物研究, 2021, 41(3):429−435. ZHANG X, ZHANG X J, MA Y P, et al. Genetic diversity assessment of Rhododendron sinofalconeri with genotyping by sequencing(GBS) [J]. Bulletin of Botanical Research, 2021, 41(3): 429−435.(in Chinese)
[22]	翟秀明, 李解, 唐敏, 等. 重庆30份茶树种质资源农艺性状与生化成分多样性 [J]. 浙江农业学报, 2021, 33(7):1244−1255. ZHAI X M, LI J, TANG M, et al. Diversity analysis of 30 tea (Camelia sinensis) germplasm resources in Chongqing based on agronomic traits and biochemical components [J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1244−1255.(in Chinese)
[23]	唐璐, 李长乐, 葛悦, 等. 茶树地方群体种资源叶片表型及生化组分多样性分析 [J]. 茶叶科学, 2023, 43(4):473−488. TANG L, LI C L, GE Y, et al. Diversity analysis of leaf phenotype and biochemical components in tea local population resources [J]. Journal of Tea Science, 2023, 43(4): 473−488.(in Chinese)
[24]	胡亮, 孙伟, 马月辉. 藏系绵羊群体遗传多样性及遗传结构分析 [J]. 畜牧兽医学报, 2019, 50(6):1145−1153. HU L, SUN W, MA Y H. Study on genetic diversity and genetic structure of Tibetan sheep populations [J]. Chinese Journal of Animal and Veterinary Sciences, 2019, 50(6): 1145−1153.(in Chinese)
[25]	杨军, 孔祥瑞, 王让剑. 基于EST-SSR标记的福建云霄县和尤溪县野生茶树遗传多样性分析 [J]. 茶叶学报, 2022, 63(1):11−19. doi: 10.3969/j.issn.1007-4872.2022.01.003 YANG J, KONG X R, WANG R J. EST-SSR marker-based genetic diversity analysis on wild tea germplasms from Yunxiao and Youxi Counties in Fujian [J]. Acta Tea Sinica, 2022, 63(1): 11−19.(in Chinese) doi: 10.3969/j.issn.1007-4872.2022.01.003
[26]	黄寿辉, 温立香, 彭静茹, 等. 广西部分地区野生茶树遗传关系EST-SSR标记分析 [J]. 广西植物, 2019, 39(6):821−830. doi: 10.11931/guihaia.gxzw201809022 HUANG S H, WEN L X, PENG J R, et al. Genetic relationship analysis of wild tea tree germplasm resources in part of Guangxi based on EST-SSR markers [J]. Guihaia, 2019, 39(6): 821−830.(in Chinese) doi: 10.11931/guihaia.gxzw201809022
[27]	崔蕾, 谢从新, 李艳和, 等. 斑点叉尾鮰4个群体遗传多样性的微卫星分析 [J]. 华中农业大学学报, 2012, 31(6):744−751. doi: 10.3969/j.issn.1000-2421.2012.06.015 CUI L, XIE C X, LI Y H, et al. Analysis of genetic diversity among four different channel catfish populations by using microsatellite markers [J]. Journal of Huazhong Agricultural University, 2012, 31(6): 744−751.(in Chinese) doi: 10.3969/j.issn.1000-2421.2012.06.015
[28]	SVED J A. The stability of linked systems of loci with a small population size [J]. Genetics, 1968, 59(4): 543−563. doi: 10.1093/genetics/59.4.543
[29]	HARTL D L, CLARK A G. Principles of population genetics[M]. 3rd ed. Sunderland, Mass. : Sinauer Associates, 1997.
[30]	BROWN A H D. Plant population genetics, breeding, and genetic resources[M]. Sunderland, Mass. : Sinauer Associates, 1990.
[31]	BRUNI I, DE MATTIA F, LABRA M, et al. Genetic variability of relict Rhododendron ferrugineumL. populations in the Northern Apennines with some inferences for a conservation strategy [J]. Plant Biosystems - an International Journal Dealing with All Aspects of Plant Biology, 2012, 146(1): 24−32. doi: 10.1080/11263504.2011.557093
[32]	杨如兴. 图解茶树高效栽培与病虫害防治[M]. 北京: 中国农业出版社, 2022.
[33]	ANGELONI F, OUBORG N J, LEIMU R. Meta-analysis on the association of population size and life history with inbreeding depression in plants [J]. Biological Conservation, 2011, 144(1): 35−43. doi: 10.1016/j.biocon.2010.08.016