Isolation and Identification of a New Duck Adenovirus B2 with Insertion and Deletion Mutations

GUO Bolun; LI Yaru; ZHENG Xin; JIANG Dandan; ZENG Li; CHEN Shaoying; MA Yanmei; CHEN Shilong

doi:10.19303/j.issn.1008-0384.2024.02.001

Volume 39 Issue 2

Feb. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(2): 125-130

GUO B L, LI Y R, ZHENG X, et al. Isolation and Identification of a New Duck Adenovirus B2 with Insertion and Deletion Mutations [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：125−130 doi: 10.19303/j.issn.1008-0384.2024.02.001

Citation:

GUO B L, LI Y R, ZHENG X, et al. Isolation and Identification of a New Duck Adenovirus B2 with Insertion and Deletion Mutations [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：125−130 doi: 10.19303/j.issn.1008-0384.2024.02.001

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Isolation and Identification of a New Duck Adenovirus B2 with Insertion and Deletion Mutations

doi: 10.19303/j.issn.1008-0384.2024.02.001

GUO Bolun^{1, 2
,},
LI Yaru^{1, 2},
ZHENG Xin^{1, 2},
JIANG Dandan^{2, 3},
ZENG Li^{2, 3},
CHEN Shaoying^{2, 3},
MA Yanmei^{1
,
,},
CHEN Shilong^{2, 3
,
,}

1.
College of Animal Sciences, Fujian Agricultural and Forestry University/Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, Fuzhou, Fujian　350002, China
2.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian　350013, China
3.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian　350003, China

Received Date: 2024-01-01
Rev Recd Date: 2024-02-01

Available Online: 2024-03-28

Publish Date: 2024-02-28

Abstract

Abstract

Objective A previously unknown strain of duck adenovirus B2 (DAdV B2) was isolated from a ducking of pale liver disease in Fujian and identified for epidemiological reference. Methods The virus associated with the disease was detected by PCR and isolated for identification by genome sequencing and a challenge test. Result The DAdV B2/BG48 virus infected LMH cells in the duck became swollen like a ballon, and eventually, died and disintegrated. The MDEF cells inoculated with the isolate changed from a spindle-shape to ball-like and clustered together with intercellular spaces in between. Distinctively, BG48 genome had a 3 bp insertion in the pX region, a 33 bp insertion in the ORF19B domain, and 42 bp deletion at the junction between ORF64 and ORF67. A G-base was found on the 133^rd spot after the starting code of ORF67 and no truncated mutation in the terminal nor other codes compared with CH-GD-12-2014, or BG27 and BG18 previously identified. In a challenge test, BG48 induced a 50% morbidity and 0 mortality on 2-day-old Muscovy ducks with similar clinical symptoms and pathological changes between the artificially infected and the naturally diseased birds. Conclusion A novel DAdV B2 strain, BG48, from a Muscovy duck of pale liver disease was isolated and identified. The virus was uniquely characterized by multiple insertions and deletion mutations in the gene suggesting a potential of causing complex epidemic.
- Pale liver disease,
- duck adenovirus B2,
- isolation and identification,
- Insertion and deletion,
- ORF67

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References(17)

References

[1]	陈仕龙, 俞博, 肖世峰, 等. 鸭2型腺病毒感染导致番鸭“肝白化病” 的研究 [J]. 福建农业科技, 2017, (8):1−3. CHEN S L, YU B, XIAO S F, et al. Primary study on duck adenovirus 2 infection resulted in Muscovy ducklings' pale liver disease [J]. Fujian Agricultural Science and Technology, 2017(8): 1−3. (in Chinese)
[2]	SHI X J, ZHANG X Y, SUN H W, et al. Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China [J]. Poultry Science, 2022, 101(1): 101564. doi: 10.1016/j.psj.2021.101564
[3]	BOUQUET J F, MOREAU Y, MCFERRAN J B, et al. Isolation and characterisation of an adenovirus isolated from Muscovy ducks [J]. Avian Pathology:Journal of the W V P A, 1982, 11(2): 301−307. doi: 10.1080/03079458208436102
[4]	MAREK A, KAJÁN G L, KOSIOL C, et al. Complete genome sequences of pigeon adenovirus 1 and duck adenovirus 2 extend the number of species within the genus Aviadenovirus [J]. Virology, 2014, 462/463: 107−114. doi: 10.1016/j.virol.2014.04.033
[5]	ZHANG X H, ZHONG Y J, ZHOU Z H, et al. Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in 2014 [J]. Virology, 2016, 493: 12−21. doi: 10.1016/j.virol.2016.03.004
[6]	CHEN S L, LIN F Q, JIANG B, et al. Isolation and characterization of a novel strain of duck aviadenovirus B from Muscovy ducklings with acute hepatitis in China [J]. Transboundary and Emerging Diseases, 2022, 69(5): 2769−2778. doi: 10.1111/tbed.14428
[7]	YIN L J, ZHOU Q, MAI K J, et al. Epidemiological investigation of duck adenovirus 3 in Southern China, during 2018-2020 [J]. Avian Pathology:Journal of the W V P A, 2022, 51(2): 171−180. doi: 10.1080/03079457.2022.2034737
[8]	CHU L L, YE S Q, WANG J Y, et al. An insertion and deletion mutant of adenovirus in Muscovy ducks [J]. Archives of Virology, 2022, 167(9): 1879−1883. doi: 10.1007/s00705-022-05474-y
[9]	徐彬. 鸭腺病毒3型(DAdV-3)对鸡致病特性的鉴定[D]. 合肥: 安徽农业大学, 2023. XU B. Identification of pathogenic properties of duck adenovirus type 3(DAdV-3)in chickens[D]. Hefei: Anhui Agricultural University, 2023. (in Chinese)
[10]	江丹丹, 林昶, 黄志坚, 等. 鸭腺病毒B血清1型和血清2型双重PCR检测方法的建立及应用 [J]. 中国预防兽医学报, 2022, 44(8):855−860. JIANG D D, LIN C, HUANG Z J, et al. Establishment and application of duplex PCR assay for detection of duck adenovirus B serotype 1 and serotype 2 [J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(8): 855−860. (in Chinese)
[11]	ROWE W P, HUEBNER R J, GILMORE L K, et al. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture [J]. Proceedings of the Society for Experimental Biology and Medicine, 1953, 84(3): 570−573. doi: 10.3181/00379727-84-20714
[12]	赵蕾, 史爱华, 李林, 等. Ⅰ群4型禽腺病毒DC株在LMH细胞的增殖工艺研究 [J]. 中国家禽, 2018, 40(20):18−22. ZHAO L, SHI A H, LI L, et al. Proliferation of group Ⅰ fowl adenovirus serotype 4 DC strain in LMH cells [J]. China Poultry, 2018, 40(20): 18−22. (in Chinese)
[13]	ANDERSON C W, YOUNG M E, FLINT S J. Characterization of the adenovirus 2 virion protein, mu [J]. Virology, 1989, 172(2): 506−512. doi: 10.1016/0042-6822(89)90193-1
[14]	LEE T W R, LAWRENCE F J, DAUKSAITE V, et al. Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins[J]. The Journal of General Virology, 2004, 85(Pt 1): 185-196.
[15]	ZHAO M L, DUAN X Y, WANG Y Q, et al. A novel role for PX, a structural protein of fowl adenovirus serotype 4 (FAdV4), as an apoptosis-inducer in leghorn male hepatocellular cell [J]. Viruses, 2020, 12(2): 228. doi: 10.3390/v12020228
[16]	李小凤, 韦悠, 罗思思, 等. X蛋白(pX)在血清4型禽腺病毒感染LMH细胞后对Toll样受体的影响 [J]. 西南农业学报, 2023, 36(12):2814−2821. LI X F, WEI Y, LUO S S, et al. Effect of X protein (pX) on Toll-like receptors after LMH cell infection with fowl adenovirus serotype 4 [J]. Southwest China Journal of Agricultural Sciences, 2023, 36(12): 2814−2821. (in Chinese)
[17]	PEI Y L, CORREDOR J C, KRELL P J, et al. Fowl adenovirus 9 ORF19, a lipase homolog, is nonessential for virus replication and is suitable for foreign gene expression [J]. Virus Research, 2019, 260: 129−134. doi: 10.1016/j.virusres.2018.12.001