Identification of S Genes and <i>SFB4'</i> in <i>Cerasus avium</i> L.

ZHANG Haonan; LI Peihua; LIANG Dong; WANG Jin; LV Xiulan

doi:10.19303/j.issn.1008-0384.2024.04.006

Volume 39 Issue 4

Apr. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(4): 418-426

ZHANG H N, LI P H, LIANG D, et al. Identification of S Genes and SFB4' in Cerasus avium L. [J]. Fujian Journal of Agricultural Sciences，2024，39（4）：418−426 doi: 10.19303/j.issn.1008-0384.2024.04.006

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ZHANG H N, LI P H, LIANG D, et al. Identification of S Genes and SFB4' in Cerasus avium L. [J]. Fujian Journal of Agricultural Sciences，2024，39（4）：418−426 doi: 10.19303/j.issn.1008-0384.2024.04.006

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Identification of S Genes and SFB4' in Cerasus avium L.

doi: 10.19303/j.issn.1008-0384.2024.04.006

College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan　611130, China

Received Date: 2023-11-03
Rev Recd Date: 2024-03-20

Available Online: 2024-05-17

Publish Date: 2024-04-28

Abstract

Abstract

Objective Varieties of sweet cherry cultivars from Sichuan Province were analyzed for self-compatibility by the presence of mutant S gene. Method Using the universal and specific primers of S genotype, DNA from the leaves of 39 Cerasus avium L. specimens collected in Sichuan were amplified by PCR. The amplified fragments containing SFB4 and/or SFB4' were sequenced for comparison and further analysis. Result Six genes of S genotype were identified from the 39 sweet cherry specimens with 27 of them having S3 genes, 19 having S9, 12 having S1, 11 having S4, 7 having S6, and 3 having S2. In the 11 specimens containing S4 genes, the ORF sequences of SFBs were amplified. By comparing SFB4 and SFB4', 4 base deletions at 903 bp were observed on the mutant. Subsequently, with a specially designed primer for SFB4', the sequencing indicated the SFB in 7 of the specimens to be SFB4'. Conclusion Six S genes were identified in the 39 C. avium specimens collected in Sichuan. The S4 from 7 of the specimens was the mutant gene, SFB4'.
- Sweet cherry,
- self-incompatibility,
- S-RNase,
- SFB

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References(22)

References

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