Preparation and <i>In Vitro</i> Inhibitory Effects of Specific Tripple Anti-vibrio Egg Yolk Antibody

ZHANG Linpeng; HONG Yucong; SUN Kaihui; ZHAO Shuyan; LIU Lihan; LIN Li; QIN Zhendong

doi:10.19303/j.issn.1008-0384.2024.08.002

Volume 39 Issue 8

Aug. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(8): 888-897

ZHANG L P, HONG Y C, SUN K H, et al. Preparation and In Vitro Inhibitory Effects of Specific Tripple Anti-vibrio Egg Yolk Antibody [J]. Fujian Journal of Agricultural Sciences，2024，39（8）：888−897 doi: 10.19303/j.issn.1008-0384.2024.08.002

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ZHANG L P, HONG Y C, SUN K H, et al. Preparation and In Vitro Inhibitory Effects of Specific Tripple Anti-vibrio Egg Yolk Antibody [J]. Fujian Journal of Agricultural Sciences，2024，39（8）：888−897 doi: 10.19303/j.issn.1008-0384.2024.08.002

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Preparation and In Vitro Inhibitory Effects of Specific Tripple Anti-vibrio Egg Yolk Antibody

doi: 10.19303/j.issn.1008-0384.2024.08.002

1.
College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering/Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou, Guangdong　510225, China
2.
Guangdong Provincial Key Laboratory of Aquatic Larvae Feed, Guangdong Yuequn Ocean Biotechnology Co., Ltd., Jieyang, Guangdong　515500, China

Received Date: 2024-02-29
Rev Recd Date: 2024-06-06

Available Online: 2024-11-13

Publish Date: 2024-08-28

Abstract

Abstract

Objective In vitro inhibitory effects of the prepared specific anti-vibrio antibody on Vibrio vulnificus, V. fluvialis, and V. cholerae were studied for the development of a bioagent to prevent and treat infections caused by the viruses. Method Trivalent inactivated vaccine against the three specific viruses were prepared to immunize hens, and eggs collected for producing the specific IgY. Potency, purity, and specificity of the IgY were determined. Antiviral effects of the antibody were tested by immunofluorescence electron microscopy, scanning electron microscopy, and an in vitro test. Result Indirect ELISA detected the specific IgY with peak titers as high as 1:51200 that sustained for 4–5 weeks when the target viruses were used as antigens. The SDS-PAGE analysis on the IgY purified by means of water dilution and salt precipitation showed a gradual rise in purity, reduction in protein impurities, and increase in visible heavy and light bands. The immunoblotting of the specific IgY showed its binding to the corresponding antigens with appearance of the protein bands. The immunofluorescence electron microscope displayed apparent agglutination on the bindings to the three viruses. And furthermore, a scanning electron microscopic image of the viruses treated with the specific IgY detailed the adhesion of the antibody to and structural disruption of the virus cells. Finally, significant in vitro, dose-dependent inhibitory effects of the specific IgY on the growth and proliferation of target vibrio strains were observed. Conclusion The prepared specific triple anti-vibrio egg yolk antibody exhibited significant in vitro inhibitory effects on V. vulnificus, V. fluvialis, and V. cholerae. It seemed plausible that the application could become an effective alternative to antibiotic treatment in preventing and controlling the diseases caused by these viruses.
- egg yolk antibody,
- Vibrio vulnificus,
- Vibrio fluvialis,
- Vibrio cholerae,
- inhibition

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References(47)

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