Physicochemical Properties and Microbial Community of Soil and Crop Yield under Rice-Tobacco-Milk Vetch Rotation Cropping

WU Tianyi; GU Gang; ZHANG Binghui; ZHANG Zongping; CHI Guosheng; WANG Ruiqi; LI Tong; CHEN Rui; XIE Xiaofang

doi:10.19303/j.issn.1008-0384.2024.08.012

Volume 39 Issue 8

Aug. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(8): 984-992

WU T Y, GU G, ZHANG B H, et al. Physicochemical Properties and Microbial Community of Soil and Crop Yield under Rice-Tobacco-Milk Vetch Rotation Cropping [J]. Fujian Journal of Agricultural Sciences，2024，39（8）：984−992 doi: 10.19303/j.issn.1008-0384.2024.08.012

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WU T Y, GU G, ZHANG B H, et al. Physicochemical Properties and Microbial Community of Soil and Crop Yield under Rice-Tobacco-Milk Vetch Rotation Cropping [J]. Fujian Journal of Agricultural Sciences，2024，39（8）：984−992 doi: 10.19303/j.issn.1008-0384.2024.08.012

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Physicochemical Properties and Microbial Community of Soil and Crop Yield under Rice-Tobacco-Milk Vetch Rotation Cropping

doi: 10.19303/j.issn.1008-0384.2024.08.012

1.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Tobacco Science, Fujian Provincial Tobacco Monopoly Bureau, Fuzhou, Fujian　350003, China
3.
Guangze Branch of Nanping Tobacco Company, Nanping, Fujian　353000, China
4.
Fujian Provincial Key Laboratory of Crop Design and Breeding, Fuzhou, Fujian　350002, China

Received Date: 2024-03-19
Rev Recd Date: 2024-05-16

Available Online: 2024-11-13

Publish Date: 2024-08-28

Abstract

Abstract

Objective Regulatory functions of milk vetch on soil fertility and microbial communities were studied to determine the potential of incorporating the shrub plant in rotation cropping with tobacco and rice for further land use improvement. Method Soil samples were collected from a field practicing tobacco-rice-milk vetch rotation cropping for 5 years and one of tobacco-rice as control. Physicochemical analysis on the soil using spectrophotometry and metagenomic sequencing on the microbial community were conducted. Result With milk vetch added to the tobacco-rice rotation cropping, the field soil increased significantly on the available nitrogen and phosphorus, rose slightly on the organic matter, total nitrogen, and total potassium, maintained a same level of total phosphorus, and reduced significantly on the available potassium. The yield of tobacco rose 2.74% and that of rice 4.67% in 2023. And the microbial diversity became significantly enriched by 8.67% and 3.10% but declined by 11.57% over control on the dominant kingdoms of Proteobacteria, Nitrospira, and Acidobacteria, respectively. The microbes in the soil were largely associated with carbohydrate, energy, and amino acid metabolisms. Conclusion By incorporating milk vetch in the rotation cropping of tobacco and rice, aside from the increased yields on the crops, the physiochemical properties of field soil were significantly improved as well.
- Milk vetch,
- rotation cropping,
- microorganisms,
- metagenomic,
- soil physicochemical properties

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References(42)

References

[1]	唐治喜, 高菊生, 宋阿琳, 等. 用宏基因组学方法研究绿肥对水稻根际微生物磷循环功能基因的影响 [J]. 植物营养与肥料学报, 2020, 26(9):1578−1590. doi: 10.11674/zwyf.20052 TANG Z X, GAO J S, SONG A L, et al. Impact of green manure on microbial phosphorus cycling genes in rice rhizosphere as investigated by metagenomics [J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(9): 1578−1590. (in Chinese) doi: 10.11674/zwyf.20052
[2]	李科江, 张素芳, 贾文竹, 等. 半干旱区长期施肥对作物产量和土壤肥力的影响 [J]. 植物营养与肥料学报, 1999, 5(1):21−25. doi: 10.3321/j.issn:1008-505X.1999.01.004 LI K J, ZHANG S F, JIA W Z, et al. Effect of long term fertilization on crop yield and soil fertility in semi arid area [J]. Journal of Plant Nutrition and Fertilizers, 1999, 5(1): 21−25. (in Chinese) doi: 10.3321/j.issn:1008-505X.1999.01.004
[3]	高菊生, 徐明岗, 董春华, 等. 长期稻-稻-绿肥轮作对水稻产量及土壤肥力的影响 [J]. 作物学报, 2013, 39(2):343−349. doi: 10.3724/SP.J.1006.2013.00343 GAO J S, XU M G, DONG C H, et al. Effects of long-term rice-rice-green manure cropping rotation on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2013, 39(2): 343−349. (in Chinese) doi: 10.3724/SP.J.1006.2013.00343
[4]	叶协锋, 杨超, 李正, 等. 绿肥对植烟土壤酶活性及土壤肥力的影响 [J]. 植物营养与肥料学报, 2013, 19(2):445−454. doi: 10.11674/zwyf.2013.0222 YE X F, YANG C, LI Z, et al. Effects of green manure in corporation on soil enzyme activities and fertility in tobacco-planting soils [J]. Plant Nutrition and Fertilizer Science, 2013, 19(2): 445−454. (in Chinese) doi: 10.11674/zwyf.2013.0222
[5]	王秀呈. 稻—稻—绿肥长期轮作对水稻土壤及根系细菌群落的影响[D]. 北京: 中国农业科学院, 2015. WANG X C. Effects of long-term rice-rice-green manure rotation on bacterial communities in rice soil and root system[D]. Beijing: Chinese Academy of Agricultural Sciences, 2015. (in Chinese)
[6]	方宇, 王飞, 贾宪波, 等. 绿肥配施减量化肥对土壤固氮菌群落的影响 [J]. 农业环境科学学报, 2018, 37(9):1933−1941. doi: 10.11654/jaes.2018-0509 FANG Y, WANG F, JIA X B, et al. Effect of green manure and reduced chemical fertilizer load on the community of soil nitrogen-fixing bacteria [J]. Journal of Agro-Environment Science, 2018, 37(9): 1933−1941. (in Chinese) doi: 10.11654/jaes.2018-0509
[7]	林多胡, 顾荣申. 中国紫云英[M]. 福州: 福建科学技术出版社, 2000.
[8]	KIM D J, CHUNG D S, BAI S C C, et al. Effects of soil selenium supplementation level on selenium contents of green tea leaves and milk vetch [J]. Preventive Nutrition and Food Science, 2007, 12(1): 35−39. doi: 10.3746/jfn.2007.12.1.035
[9]	ASAGI N, UENO H. Nitrogen dynamics in paddy soil applied with various 15N-labelled green manures [J]. Plant and Soil, 2009, 322(1): 251−262.
[10]	周影, 魏启舜, 管永祥, 等. 播种量对晚播紫云英生长及养分积累的效应 [J]. 土壤, 2020, 52(3):482−486. ZHOU Y, WEI Q S, GUAN Y X, et al. Effects of different sowing rates on growth and nutrient accumulation of later-sown Chinese milk vetch [J]. Soils, 2020, 52(3): 482−486. (in Chinese)
[11]	谢志坚, 周春火, 贺亚琴, 等. 21世纪我国稻区种植紫云英的研究现状及展望 [J]. 草业学报, 2018, 27(8):185−196. doi: 10.11686/cyxb2017360 XIE Z J, ZHOU C H, HE Y Q, et al. A review of Astragalus sinicus in paddy fields in South China since 2000s [J]. Acta Prataculturae Sinica, 2018, 27(8): 185−196. (in Chinese) doi: 10.11686/cyxb2017360
[12]	黄晶, 刘淑军, 张会民, 等. 水稻产量对双季稻-不同冬绿肥轮作及环境的响应 [J]. 生态环境学报, 2016, 25(8):1271−1276. HUANG J, LIU S J, ZHANG H M, et al. The response of rice yields on long-term double cropping rice with different winter green manure rotation and environment [J]. Ecology and Environmental Sciences, 2016, 25(8): 1271−1276. (in Chinese)
[13]	XIE Z J, TU S X, SHAH F, et al. Substitution of fertilizer-N by green manure improves the sustainability of yield in double-rice cropping system in South China [J]. Field Crops Research, 2016, 188: 142−149. doi: 10.1016/j.fcr.2016.01.006
[14]	谢志坚, 涂书新, 徐昌旭, 等. 紫云英还田对单季稻田氨挥发的影响 [J]. 核农学报, 2017, 31(8):1576−1584. doi: 10.11869/j.issn.100-8551.2017.08.1576 XIE Z J, TU S X, XU C X, et al. Effects of Chinese milk vetch on ammonia volatilization from single season rice fields in South China [J]. Journal of Nuclear Agricultural Sciences, 2017, 31(8): 1576−1584. (in Chinese) doi: 10.11869/j.issn.100-8551.2017.08.1576
[15]	马守田, 冯荣成, 张黛静, 等. 有机物料替代部分氮肥对小麦光合特性及产量的影响 [J]. 河南农业科学, 2015, 44(2):48−51. MA S T, FENG R C, ZHANG D J, et al. Effects of replacing part of nitrogen fertilizer by organic materials on photosynthetic traits and yield of wheat [J]. Journal of Henan Agricultural Sciences, 2015, 44(2): 48−51. (in Chinese)
[16]	周国朋, 谢志坚, 曹卫东, 等. 稻草高茬-紫云英联合还田改善土壤肥力提高作物产量 [J]. 农业工程学报, 2017, 33(23):157−163. doi: 10.11975/j.issn.1002-6819.2017.23.020 ZHOU G P, XIE Z J, CAO W D, et al. Co-incorporation of high rice stubble and Chinese milk vetch improving soil fertility and yield of rice [J]. Transactions of the Chinese Society of Agricultural Engineering, 2017, 33(23): 157−163. (in Chinese) doi: 10.11975/j.issn.1002-6819.2017.23.020
[17]	程会丹, 鲁艳红, 聂军, 等. 减量化肥配施紫云英对稻田土壤碳、氮的影响 [J]. 农业环境科学学报, 2020, 39(6):1259−1270. doi: 10.11654/jaes.2019-1356 CHENG H D, LU Y H, NIE J, et al. Effects of reducing chemical fertilizer combined with Chinese milk vetch on soil carbon and nitrogen in paddy fields [J]. Journal of Agro-Environment Science, 2020, 39(6): 1259−1270. (in Chinese) doi: 10.11654/jaes.2019-1356
[18]	刘小粉, 贺小思, 易柏宁, 等. 有机肥绿肥配施对水稻土有机碳组分和水稻产量的影响 [J]. 中国土壤与肥料, 2020, (5):147−151. doi: 10.11838/sfsc.1673-6257.20045 LIU X F, HE X S, YI B N, et al. Effect of applying organic fertilizer and green manure on organic carbon fractions and rice yield in a paddy soil [J]. Soil and Fertilizer Sciences in China, 2020(5): 147−151. (in Chinese) doi: 10.11838/sfsc.1673-6257.20045
[19]	杨曾平, 高菊生, 郑圣先, 等. 长期冬种绿肥对红壤性水稻土微生物特性及酶活性的影响 [J]. 土壤, 2011, 43(4):576−583. YANG Z P, GAO J S, ZHENG S X, et al. Effects of long-term winter planting-green manure on microbial properties and enzyme activities in reddish paddy soil [J]. Soils, 2011, 43(4): 576−583. (in Chinese)
[20]	张珺穜, 曹卫东, 徐昌旭, 等. 种植利用紫云英对稻田土壤微生物及酶活性的影响 [J]. 中国土壤与肥料, 2012, (1):19−25. doi: 10.3969/j.issn.1673-6257.2012.01.004 ZHANG J T, CAO W D, XU C X, et al. Effects of incorporation of milk vetch(Astragalus sinicus)on microbial populations and enzyme activities of paddy soil in Jiangxi [J]. Soil and Fertilizer Sciences in China, 2012(1): 19−25. (in Chinese) doi: 10.3969/j.issn.1673-6257.2012.01.004
[21]	肖嫩群, 张洪霞, 成壮, 等. 紫云英还田量对烟田土壤微生物及酶的影响 [J]. 中国生态农业学报, 2010, 18(4):711−715. doi: 10.3724/SP.J.1011.2010.00711 XIAO N Q, ZHANG H X, CHENG Z, et al. Effect of incorporation of Astragalus sinicus on microbe and enzyme dynamics in tobacco cultivated soils [J]. Chinese Journal of Eco-Agriculture, 2010, 18(4): 711−715. (in Chinese) doi: 10.3724/SP.J.1011.2010.00711
[22]	FALKOWSKI P G, FENCHEL T, DELONG E F. The microbial engines that drive Earth’s biogeochemical cycles [J]. Science, 2008, 320(5879): 1034−1039. doi: 10.1126/science.1153213
[23]	万水霞, 唐杉, 王允青, 等. 紫云英还田量对稻田土壤微生物数量及活度的影响 [J]. 中国土壤与肥料, 2013, (4):39−42. WAN S X, TANG S, WANG Y Q, et al. Effect of returning quantity of Astragalus sinicus to soil on quantity and activity of microbial in paddy soil [J]. Soil and Fertilizer Sciences in China, 2013(4): 39−42. (in Chinese)
[24]	ZHANG X X, ZHANG R J, GAO J S, et al. Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria [J]. Soil Biology and Biochemistry, 2017, 104: 208−217. doi: 10.1016/j.soilbio.2016.10.023
[25]	李增强, 张贤, 王建红, 等. 紫云英施用量对土壤活性有机碳和碳转化酶活性的影响 [J]. 中国土壤与肥料, 2018, (4):14−20. doi: 10.11838/sfsc.20180403 LI Z Q, ZHANG X, WANG J H, et al. Effects of Chinese milk vetch (Astragalus sinicus L. ) application rate on soil labile organic carbon and C-transformation enzyme activities [J]. Soil and Fertilizer Sciences in China, 2018(4): 14−20. (in Chinese) doi: 10.11838/sfsc.20180403
[26]	朱强, 张静, 郭再华, 等. 稻草和紫云英联合还田下施氮水平对水稻产量及土壤氮素形态的影响 [J]. 植物营养与肥料学报, 2020, 26(12):2177−2183. doi: 10.11674/zwyf.20384 ZHU Q, ZHANG J, GUO Z H, et al. Effects of different nitrogen inputs on rice yield and soil nitrogen forms under incorporation of rice straw and Chinese milk vetch [J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(12): 2177−2183. (in Chinese) doi: 10.11674/zwyf.20384
[27]	WANG Y F, LIU X M, BUTTERLY C, et al. pH change, carbon and nitrogen mineralization in paddy soils as affected by Chinese milk vetch addition and soil water regime [J]. Journal of Soils and Sediments, 2013, 13(4): 654−663. doi: 10.1007/s11368-012-0645-3
[28]	童跃伟, 屈利利, 符庆响, 等. 大别山南坡森林植物群落物种多样性及其与海拔因子的关系 [J]. 生态学报, 2024, 44(12):5307−5317. TONG Y W, QU L L, FU Q X, et al. Species diversity of forest plant communities on the southern slope of the Dabie Mountains and its relationship with altitude factors [J]. Acta Ecologica Sinica, 2024, 44(12): 5307−5317. (in Chinese)
[29]	颜志雷, 方宇, 陈济琛, 等. 连年翻压紫云英对稻田土壤养分和微生物学特性的影响 [J]. 植物营养与肥料学报, 2014, 20(5):1151−1160. doi: 10.11674/zwyf.2014.0511 YAN Z L, FANG Y, CHEN J C, et al. Effect of turning over Chinese milk vetch(Astragalus sinicus L.) on soil nutrients and microbial properties in paddy fields [J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(5): 1151−1160. (in Chinese) doi: 10.11674/zwyf.2014.0511
[30]	聂良鹏, 郭利伟, 郑春风, 等. 种植翻压紫云英配施化肥对稻田土壤理化性状和水稻产量的影响 [J]. 中国农学通报, 2021, 37(27):65−69. doi: 10.11924/j.issn.1000-6850.casb2020-0767 NIE L P, GUO L W, ZHENG C F, et al. Planting and incorporation of Chinese milk vetch coupled with chemical fertilizer application: Effects on the physical and chemical characters of paddy soil and rice yield [J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 65−69. (in Chinese) doi: 10.11924/j.issn.1000-6850.casb2020-0767
[31]	王慧, 周国朋, 常单娜, 等. 湘北双季稻区种植翻压紫云英的氮肥减施效应 [J]. 植物营养与肥料学报, 2022, 28(1):33−44. doi: 10.11674/zwyf.2021292 WANG H, ZHOU G P, CHANG D N, et al. Nitrogen reduction effects in double rice by planting and returning Chinese milk vetch to the field in Northern Hunan Province [J]. Journal of Plant Nutrition and Fertilizers, 2022, 28(1): 33−44. (in Chinese) doi: 10.11674/zwyf.2021292
[32]	常单娜, 王慧, 周国朋, 等. 赣北地区稻-稻-紫云英轮作体系减施化肥对水稻产量、氮素吸收及土壤供氮能力的影响 [J]. 植物营养与肥料学报, 2023, 29(8):1449−1460. doi: 10.11674/zwyf.2022707 CHANG D N, WANG H, ZHOU G P, et al. Yield and nitrogen uptake of rice and soil nitrogen supply capacity under fertilizer reduction in a rice-rice-Chinese milk vetch rotation system, northern Jiangxi Province, China [J]. Journal of Plant Nutrition and Fertilizers, 2023, 29(8): 1449−1460. (in Chinese) doi: 10.11674/zwyf.2022707
[33]	张济世, 张琳, 丁丽, 等. 紫云英还田与化肥减量配施对土壤氮素供应和水稻生长的影响 [J]. 植物营养与肥料学报, 2022, 28(10):1793−1803. doi: 10.11674/zwyf.2022186 ZHANG J S, ZHANG L, DING L, et al. Effects of Chinese milk vetch incorporation and chemical fertilizer reduction on soil nitrogen supply and rice growth [J]. Journal of Plant Nutrition and Fertilizers, 2022, 28(10): 1793−1803. (in Chinese) doi: 10.11674/zwyf.2022186
[34]	吕玉虎, 郭晓彦, 李本银, 等. 翻压不同量紫云英配施减量化肥对土壤肥力和水稻产量的影响 [J]. 中国土壤与肥料, 2017, (5):94−98. doi: 10.11838/sfsc.20170516 LÜ Y H, GUO X Y, LI B Y, et al. Effects of the incorporation of various amounts of Chinese milk vetch (Astragalus sinicus L. ) and reducing chemical fertilizer on soil fertility and rice yield [J]. Soil and Fertilizer Sciences in China, 2017(5): 94−98. (in Chinese) doi: 10.11838/sfsc.20170516
[35]	何春梅, 钟少杰, 严建辉, 等. 紫云英翻压对葡萄产量品质与果园土壤理化性状及微生物量的影响 [J]. 福建农业学报, 2018, 33(11):1151−1157. HE C M, ZHONG S J, YAN J H, et al. Effect of Chinese milk vetch(Astragalus sinicus L. ) as a green manure on grape productivity and quality, nutrient contents, and microbiologic properties of vineyard soils [J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1151−1157. (in Chinese)
[36]	林文星, 马鹏生, 王芳, 等. 基于宏基因组学研究分析农药对宁夏枸杞地微生物的影响 [J]. 北方园艺, 2023, (1):98−105. LIN W X, MA P S, WANG F, et al. Analysis of the effects of pesticides on microbial diversity of Lycium chinense l. in Ningxia based on macrogenomics [J]. Northern Horticulture, 2023(1): 98−105. (in Chinese)
[37]	PHILIPPOT L, RAAIJMAKERS J M, LEMANCEAU P, et al. Going back to the roots: The microbial ecology of the rhizosphere [J]. Nature Reviews Microbiology, 2013, 11(11): 789−799. doi: 10.1038/nrmicro3109
[38]	刘进, 冀瑞卿, 李冠霖, 等. 红松和蒙古栎菌根系变形菌门细菌的群落多样性[J/OL]. 吉林农业大学学报, 2023: 1−9. LIU J, JI R Q, LI G L, et al. Diversity of Proteobacteria in the ectomycorrhizosphere of Pinus koraiensis and Quercus mongolica[J/OL]. Journal of Jilin Agricultural University, 2023: 1−9. (in Chinese)
[39]	钟珍梅. 圆叶决明对果园红壤可溶性氮及细菌群落动态变化的影响[D]. 福州: 福建农林大学, 2019. ZHONG Z M. Effects of Cassia rotundifolia on the dynamic changes of soluble nitrogen and bacterial community in red soil of orchard[D]. Fuzhou: Fujian Agriculture and Forestry University, 2019. (in Chinese)
[40]	赵峥, 朱元宏, 周德平, 等. 不同轮作模式对稻田土壤肥力和微生物群落结构的影响[J]. 农业环境科学学报, 2024,43(4): 874−885. ZHAO Z, ZHU Y H, ZHOU D P, et al. Effects of different rotation patterns on soil fertility and microbial community composition in a paddy field system[J]. Journal of Agro-Environment Science, 2024, 43(4): : 874−885. (in Chinese)
[41]	王新月. 磷石膏污染农田土壤中镉、氟和磷的交互作用研究[D]. 雅安: 四川农业大学, 2022. WANG X Y. Study on the interaction of cadmium, fluorine and phosphorus in agricultural soil polluted by phosphogypsum[D]. Yaan: Sichuan Agricultural University, 2022. (in Chinese)
[42]	万水霞, 朱宏斌, 唐杉, 等. 紫云英与化肥配施对稻田土壤养分和微生物学特性的影响 [J]. 中国土壤与肥料, 2015, (3):79−83. doi: 10.11838/sfsc.20150314 WAN S X, ZHU H B, TANG S, et al. Effects of Astragalus sinicus manure and fertilizer combined application on soil nutrient and microbiological characteristics [J]. Soil and Fertilizer Sciences in China, 2015(3): 79−83. (in Chinese) doi: 10.11838/sfsc.20150314