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HE Wei, LIAN Ling, XIONG Hai-qing, CAI Qiu-hua, ZHU Yong-sheng, XIE Hong-guang, WANG Ying-heng, ZHANG Jian-fu, XIE Hua-an. Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 924-930.
Citation:
HE Wei, LIAN Ling, XIONG Hai-qing, CAI Qiu-hua, ZHU Yong-sheng, XIE Hong-guang, WANG Ying-heng, ZHANG Jian-fu, XIE Hua-an. Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 924-930.
HE Wei, LIAN Ling, XIONG Hai-qing, CAI Qiu-hua, ZHU Yong-sheng, XIE Hong-guang, WANG Ying-heng, ZHANG Jian-fu, XIE Hua-an. Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 924-930.
Citation:
HE Wei, LIAN Ling, XIONG Hai-qing, CAI Qiu-hua, ZHU Yong-sheng, XIE Hong-guang, WANG Ying-heng, ZHANG Jian-fu, XIE Hua-an. Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 924-930.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China;
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding for Hybrid Rice of South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China;
3.
Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;
4.
National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China
Based on gene chip of japonica rice cv. Yunyin induced with Magnaporthe grisea, one of the candidate genes up-regulated remarkably by Sichuan43's isolation was cloned. Sequence and phylogenetic tree analyses suggest that the gene is closest relative to Oryza sativa L. AF529237.2 and Tritieum aestivum X51608.1 with 99.0% and 88.0% identity, and may relate to phosphoribulo kinase which is a critical enzyme in calvin cycle. Subsequently, the overexpression, RNAi expression and transient expression vector were constructed to obtain corresponsive transgenic lines, respectively. All these transgenic lines generated were used to assess the function of the gene in the stress response.
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[3]
RUMPHO M E,POCHAREDDY S,WORFUL J M,et al. Molecular Characterization of the Calvin Cycle Enzyme Phosphoribulokinase in the Stramenopile Alga Vaucheria litorea and the Plastid Hosting Mollusc Elysia chlorotica[J]. Molecular Plant,2009,2(6):1384-1396.
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