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Volume 24 Issue 4
Nov.  2011
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WEI Xiu-qing, CHEN Xiao-jing. Comparison of chromosome spreading methods and karyotype of Passiflora edulis Sims[J]. Fujian Journal of Agricultural Sciences, 2009, 24(4): 328-332.
Citation: WEI Xiu-qing, CHEN Xiao-jing. Comparison of chromosome spreading methods and karyotype of Passiflora edulis Sims[J]. Fujian Journal of Agricultural Sciences, 2009, 24(4): 328-332.

Comparison of chromosome spreading methods and karyotype of Passiflora edulis Sims

  • Received Date: 2009-06-25
  • Rev Recd Date: 2009-08-03
  • Publish Date: 2009-08-15
  • Effects of different pretreatments methods,dissociation,enzymolysis time and slide making on the metaphase cells,chromosome contraction and dispersion degree of P. edulis Sims were compared.The best metaphase cells were prepared after the pretreatment with 8-Hydroxyquinoline for 2.5 h at room temperature and the subsequent enzyme digestion by a mixture containing 2% cellulase and 0.5% pectinase for 70 min at 37 ℃.The chromosomes dispersed well with clean background and centromeric dots and satellite were clear.The karyotype formula of P. edulis Sims was 2n=2x=18=14m(2sat)+4sm,belonging to 2A type,and a pair of satellites were located on the forth pair chromosomes.
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  • [1]
    陈晓静,吕柳新.西番莲属2个主要栽培类型的核型分析[J].福建农业大学学报:自然科学版,1994,21(3):30-33.
    [2]
    陈晓静.福建3个产地水仙的核型分析[J].植物资源与环境学报,2004,13(4):28-31.
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