Somatic Incompatibility and Genetic Differences among Strains of <i>Ganoderma sinense</i>

JIANG Xiaohan; YANG Chunyan; WANG Chezhao; LIU Xinrui

doi:10.19303/j.issn.1008-0384.2024.05.006

Volume 39 Issue 5

May 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(5): 550-556

JIANG X H, YANG C Y, WANG C Z, et al. Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：550−556 doi: 10.19303/j.issn.1008-0384.2024.05.006

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JIANG X H, YANG C Y, WANG C Z, et al. Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense [J]. Fujian Journal of Agricultural Sciences，2024，39（5）：550−556 doi: 10.19303/j.issn.1008-0384.2024.05.006

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Somatic Incompatibility and Genetic Differences among Strains of Ganoderma sinense

doi: 10.19303/j.issn.1008-0384.2024.05.006

College of Life Sciences/Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2024-03-18
Rev Recd Date: 2024-04-10

Available Online: 2024-06-26

Publish Date: 2024-05-28

Abstract

Abstract

Objective Somatic incompatibility (SI) and genetic differences among Ganoderma sinense strains were studied. Method DikaryonG. sinense strains were generated from 7 monokaryon parents by uni- or bi-directional nuclear migration. On a PDA medium, SI between the dikaryon strains was tested, and genetic differentiations examined using ISSR, RAPD, and SRAP molecular markers. Result Eleven dikaron strains of 5 distinctive genetic types showing a gap, a gap with line, or a wall-like SI were obtained. The combined genetic similarity coefficients of the ISSR, RAPD, and SRAP molecular markers on the strains ranged from 0.29 to 0.97. The UPMGA clustering of the strains corresponded to that of the parents. Conclusion The SI among the G. sinense strains were mainly associated with the nucleus rather than the cytoplasm. Since the SI types largely paralleled the genetic differences of the dikaron strains, SI determination could be conveniently applied to preliminarily study the genetics of a G. sinense strain.
- antagonism,
- genetic relationship,
- molecular marker

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References(21)

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