Abstract: In this study, we identified 4 key genes of JA signaling pathway (COI1、PDF1.2、MYC2-1、MYC2-2) and SA signaling pathway (PR1-1、PR1-2、NPR1-1、NPR1-2) in Flammulina velutipes, respectively. The results of NBT staining and RT-qPCR demonstrated that JA/SA signaling pathways of F. velutipes responsed to exogenous JA/SA. In addition, the expression levels of these genes of JA/SA signaling pathway significantly increased under the treatment of exogenous 50 μmol · L^-1 JA and 500 μmol · L^-1 SA for 12 h, respectively. It was worth noting that PDF1.2 could be considered as the marker gene of JA signaling pathways for its response to exogenous JA obviously. The antagonistic effect between JA and SA signaling pathways may also exist in F.velutipes and the protein NPR1 of SA signaling pathways had inhibitory effect on the gene PDF1.2 expression level of JA signaling pathways which could be affected by the relative concentration of exogenous JA/SA.