刺托竹荪菌盖碳量子点的制备及其抑菌性研究

郑梅霞; 林丽萍; 刘波; 陈峥; 朱育菁

doi:10.19303/j.issn.1008-0384.2019.04.010

刺托竹荪菌盖碳量子点的制备及其抑菌性研究

Preparation, Characterization and Antimicrobial Activity of Carbon Dots from Pilei of Dictyophora echinovolvata

摘要

摘要:
目的利用废旧生物质资源制备量子点并增加细菌病害防治的资源。
方法以刺托竹荪菌盖采用水热法合成碳量子点，并用透射电镜扫描、红外光谱、XRD和荧光分析对碳量子点进行表征。选取青枯雷尔氏菌Ralstonia solanacearum和地毯草黄单胞菌Xanthomonas axonopodis作为供试菌种，采用抑菌圈法对碳量子点的抑菌性能进行研究。
结果该方法制得的碳量子点近似球形，粒径均匀，分散性好。其发射波长依赖于激发波长，在紫外灯的照射下，出现明亮的蓝色荧光。碳量子点对青枯雷尔氏菌和地毯草黄单胞菌均有明显的抑菌作用。
结论获得了一种可用于防治植物细菌病害的量子点。

Abstract:
Objective Utilization of mushroom biomass waste to prepare carbon dots (CDs) for the prevention and control of diseases on plants was studied.
Method CDs were obtained by hydrothermal oxidation of pilei of Dictyophora echinovolvata and characterized by the transmission electron microscopy (TEM), Fourier transform infrared spectrophoto-metry (FT-IR), x-ray diffraction (XRD), and fluorescene spectrophotometry. Antibacterial properties of the CDs were determined by measurements of diameter of the bacteriostatic circle on Ralstonia solanacearum and Xanthomonas axonopodis.
Result The CDs obtained displayed an appropriate spherical morphology and uniform size with good dispersion. They emitted bright blue photoluminescence under UV excitation, exhibited an excitation-dependent PL behavior, and demonstrated excellent antibacterial activities against R. solanacearum and X. axonopodis.
Conclusion The CDs with a potential of preventing and controlling plant diseases by bacterial infection were successful prepared in laboratory.

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