金线莲3个持家基因表达稳定性分析

Expression Stabilities of Three Housekeeping Genes of Anoectochilus roxburghii

摘要: 从金线莲转录组数据中筛选了甘油醛-3-磷酸脱氢酶基因（GenBank登录号：MH899010）、肌动蛋白基因（GenBank登录号：MH899011）和α-微管蛋白基因（GenBank登录号：MH899012）3个Unigenes，以不同温度处理和不同生长期的金线莲样品为材料，分析3个基因的表达稳定性。结果表明：甘油醛-3-磷酸脱氢酶基因和α-微管蛋白基因的表达受温度变化的影响，且不同生长期表达量也存在差异；肌动蛋白基因在茎、叶中的表达量基本一致，表达稳定，不受温度变化和生长期的影响。分析温度处理和生长期金线莲功能基因表达水平可选择肌动蛋白基因作为内参基因。

Abstract: Three unigenes of GAPDH (GenBank accession number:MH899010), Actin (GenBank accession number:MH899011) and α-tubulin (GenBank accession number:MH899012) were selected from Anoectochilus roxburghii transcriptome data. Their expression stabilities under various temperatures at different plant growth stages were studied. The results indicated that the expressions of GAPDH and α-tubulin genes varied under the test conditions, but that of Actin in the A. roxburghii stems and leaves was stable and consistent. It appeared that the gene of Actin would be a plausible candidate to be used as a reference in analyzing the expression of functional genes under varied temperatures during different growth stages.