外源脱落酸对干旱胁迫下云南山茶幼苗内源激素代谢的影响

王魏沁澜; 林先玉; 晓林; 周麟; 付鲜; 屈燕

doi:10.19303/j.issn.1008-0384.2024.08.008

外源脱落酸对干旱胁迫下云南山茶幼苗内源激素代谢的影响

doi: 10.19303/j.issn.1008-0384.2024.08.008

王魏沁澜^{1, 2, 3,},
林先玉^{1, 2, 3},
晓林^{1, 2, 3},
周麟^{1, 2, 3},
付鲜^{1, 2, 3},
屈燕^{1, 2, 3, ,}

1.
西南林业大学园林园艺学院，云南　昆明　650224
2.
国家林业和草原局西南风景园林工程技术研究中心，云南　昆明　650224
3.
云南省功能性花卉资源及产业化技术工程研究中心，云南　昆明　650224

基金项目: 国家重点研发计划项目（2019YFD1001005）；云南省万人计划青年拔尖人才项目（YNWR-QNBJ-2019-211）

详细信息

作者简介:
王魏沁澜（2000 —），女，硕士研究生，研究方向为事园林植物资源开发与利用，E-mail：1532233861@qq.com

通讯作者:
屈燕（1979 —），女，教授，博士生导师，主要从事园林植物资源开发与利用研究，E-mail：quyan@swfu.edu.cn

中图分类号: S685.14
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- 被引次数: 0
出版历程
- 收稿日期: 2024-03-19
- 修回日期: 2024-04-23
- 网络出版日期: 2024-11-13
- 刊出日期: 2024-08-28

Effects of Abscisic Acid Spray on Hormone Metabolism of Camellia reticulata Seedlings under Drought Stress

WANG Weiqinlan^{1, 2, 3
,},
LIN Xianyu^{1, 2, 3},
XIAO Lin^{1, 2, 3},
ZHOU Lin^{1, 2, 3},
FU Xian^{1, 2, 3},
QU Yan^{1, 2, 3
, ,}

1.
College of Landscape and Horticulture, Southwest Forestry University, Kunming, Yunnan　650224, China
2.
Southwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration), Kunming, Yunnan　650224, China
3.
Yunnan Engineering Research Center for Functional Flower Resources and Industrialization, Kunming, Yunnan　650224, China

摘要

摘要: 目的探究喷施外源脱落酸（abscisic acid, ABA）对干旱胁迫下两年生云南山茶紫袍（Camellia reticulata ‘Zipao’）幼苗根和叶内源激素的影响。方法采用100 mg·L⁻¹脱落酸对云南山茶幼苗叶面喷施，并用PEG_6000模拟干旱法进行干旱胁迫，研究在干旱胁迫及复水处理下外源ABA对云南山茶根和叶的渗透调节物质和内源激素代谢组的影响。结果干旱及复水处理下渗透调节的关键部位是在叶。干旱胁迫下云南山茶幼苗根部促进ABA、赤霉素（gibberellins, GA）、细胞分裂素（cytokinins, CKs）含量，降低生长素（auxin, IAA）含量，而叶部促进ABA、茉莉酸（jasmonates, JA）、水杨酸（salicylic acid, SA）、IAA、CKs含量的积累。复水处理下云南山茶幼苗根部ABA、GA、CKs各激素水平逐渐下降，IAA含量逐渐上升，而叶部通过积累JA、SA、IAA、CKs含量进行调控。差异显著代谢物KEGG富集分析表明，根部代谢物显著富集在二萜生物合成通路中，叶部显著富集在植物激素信号转导途径和玉米素生物合成通路中。渗透调节物质与内源激素的相关性分析表明二者之间存在显著正相关。施用外源ABA在干旱胁迫下可分别提升云南山茶根部可溶性蛋白、可溶性糖、ABA、GA、CKs和叶部游离脯氨酸、ABA、JA的含量，进而提高耐旱力；在复水期可分别降低根部CKs和叶部JA的含量，促进根部和叶部IAA的合成，进而促进植株的恢复。结论云南山茶幼苗根和叶对干旱胁迫及复水处理的响应存在差异，施用外源ABA可提高云南山茶的耐旱力。
- 云南山茶 /
- 根 /
- 叶 /
- 干旱胁迫 /
- 脱落酸 /
- 渗透调节物质 /
- 内源激素
Abstract: Objective Effects of ABA spray on endogenous hormones in Camellia reticulata seedlings under drought stress were studied. Methods An ABA solution in the concentration of 100 mg·L⁻¹ was sprayed on the leaves of 2-year-old C. reticulata Zipao seedlings grown in potting soil under a simulated drought stress using PEG_6000. Osmoregulatory substances and hormone metabolome in the roots and leaves of the plants were measured under the treatment and subsequent rehydration. Results Osmotic regulation of the seedlings took place mainly in the leaves. Under drought stress, ABA, JA , SA ,IAA, and CKs accumulated in the leaves, while ABA, GA , and CKs increased but IAA decreased in the roots. Upon rehydration, ABA, GA, and CKs in the roots gradually declined, while IAA rose, meanwhile, the leaves started regulating with the stored JA, SA, IAA, and CKs. The KEGG enrichment analysis showed that the diterpene biosynthesis pathway in the roots and the hormone signal transduction and zein biosynthesis pathways in the leaves were significantly enhanced. There was a significant correlation between the osmoregulatory substances and endogenous hormones. The ABA spray boosted the contents of soluble protein, soluble sugar, ABA, GA, and CKs in the roots as well as those of free proline, ABA, and JA in the leaves improving the drought tolerance of the plant. With replenished water, CKs in the roots and JA in the leaves were reduced to encourage IAA synthesis, which in turn, aided the recovery of plant functions. Conclusion The responses of the roots and leaves of C. reticulata seedlings to the imposed drought and the subsequent rehydration were different. Application of foliar ABA spray could improve the draught resistance of the plants.
- Camellia reticulata /
- roots /
- leaves /
- drought stress /
- abscisic acid /
- osmoregulatory substances /
- endogenous hormones

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图 1 云南山茶根和叶渗透调节物质含量

不同小写字母表示云南山茶根和叶不同时间处理间的差异显著性（P＜0.05）。不同大写字母表示同一时间云南山茶根和叶处理间的差异显著性（P＜0.05）。图5同。

Figure 1. Contents of osmoregulatory substances in roots and leaves of C. reticulata

Data with different lowercase letters indicate significant differences at sampling times (P＜0.05); those with different capital letters indicate significant differences at same sampling time (P＜0.05). Same for Fig. 5.

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图 2 内源激素代谢物种类及占比及PCA主成分分析

Figure 2. Types, proportion, and PCA principal components of endogenous hormone metabolites

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图 3 胁迫期及复水期内源激素差异代谢物韦恩图

Figure 3. Venn diagram of hormone metabolites in plant under stress and after rehydration

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图 4 差异代谢物的KEGG富集

横坐标表示每个通路对应的富集因子，纵坐标为通路名称，点的颜色为P值，越红表示富集越显著。点的大小代表富集到的差异代谢物的个数多少。a：G0 hA_vs_G72 hA，b：Y0 hA_vs_Y72 hA，c：G72 hA_vs_GR72 hA，d：Y72 hA_vs_YR72 hA。

Figure 4. KEGG enrichment map of differential metabolites

Horizontal coordinate: rich factor corresponding to each pathway; vertical coordinate: path name; dot color: P value, the deeper the red color, the more significant the enrichment; dot size: number of differentiated metabolites enriched; a: G0hA_vs_G72hA; b: Y0hA_vs_Y72hA; c: G72hA_vs_GR72hA; d: Y72hA_vs_YR72hA.

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图 5 胁迫期及复水期2种脱落酸及1种水杨酸含量的变化

Figure 5. Contents of ABAs and SA in plant under stress and after rehydration

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图 6 胁迫期及复水期3种赤霉素含量变化及茉莉酸热图

上下调图中蓝色柱状为D1组，红色柱状为D2组，蓝色方框框选的代谢物为D1组和D2组共有的差异代谢物，未框选的即为特有差异代谢物，下同。a、b、c：3种赤霉素含量变化；d：胁迫期叶片中茉莉酸热图，e：复水期叶片中茉莉酸热图。

Figure 6. GA and JA heat map of plant under stress and after rehydration

In upper and lower diagrams, blue bar: Group D1; red bar: Group D2; metabolites in blue box: differential metabolites shared by D1 and D2; unselected metabolites: unique differential metabolites. Same for below. a, b, c: contents of 3 kinds of GA; d: heat map of JA in leaves under stress; e: heat map of JA in leaves after rehydration.

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图 7 胁迫期及复水期生长素、细胞分裂素热图及上下调图

a：胁迫期根系中生长素热图，b：胁迫期根系中细胞分裂素热图，c：胁迫期叶片中生长素热图，d：胁迫期叶片中细胞分裂素热图，e：复水期期根系中生长素热图，f：复水期根系中细胞分裂素热图，g：复水期叶片中生长素热图，h：复水期叶片中细胞分裂素热图。

Figure 7. Heat and up-down maps of IAA and cytokinin in plant under stress and after rehydration

a: IAA heat map in roots under stress; b: cytokinin heat map in roots under stress; c: IAA heat map in leaves under stress; d: cytokinin heat map in leaves under stress; e: IAA heat map in roots after rehydration; f: cytokinin heat map in roots after rehydration; g: IAA heat map in leaves after rehydration; h: cytokinin heat map in leaves after rehydration.

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表 1 试验处理组别代表符号

Table 1. Symbols for various treatments

处理组 Treatment group	组织部位 Tissue site	干旱胁迫时长 Drought stress duration				复水后24 h（R24 h） 24 h after rehydration	复水后72 h（R72 h） 72 h after rehydration
处理组 Treatment group	组织部位 Tissue site	0 h	24 h	48 h	72 h	复水后24 h（R24 h） 24 h after rehydration	复水后72 h（R72 h） 72 h after rehydration
D1	根（G1）	G0 h	G24 h	G48 h	G72 h	GR24 h	GR72 h
D1	叶（Y1）	Y0 h	Y24 h	Y48 h	Y72 h	YR24 h	YR72 h
D2	根（G2）	G0 hA	G24 hA	G48 hA	G72 hA	GR24 hA	GR72 hA
D2	叶（Y2）	Y0 hA	Y24 hA	Y48 hA	Y72 hA	YR24 hA	YR72 hA

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表 2 云南山茶幼苗根部生理指标、内源激素的相关系数

Table 2. Correlation coefficient of physiological indexes and endogenous hormones of C. reticulata roots

生理指标/激素 Physiological indicators/Hormone	生理指标/激素 Physiological indicators/Hormone	相关性 Correlation
SP	IAA	0.948^**
SP	GA	0.831^*
SP	SA	0.976^**
SP	SS	0.954^*
SS	IAA	0.917^*
SS	GA	0.844^*
SS	SA	0.907^*
IAA	GA	0.917^*
IAA	SA	0.875^*
*表示极显著相关（P＜0.01），表示显著相关（P＜0.05）。下同。 *: extremely significant correlation at P＜0.01; : significant correlation at P＜0.05. Same for below.

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表 3 云南山茶幼苗叶部生理指标、内源激素间的相关系数

Table 3. Correlation coefficient between physiological indexes and endogenous hormones of C. reticulata leaves

生理指标/激素 Physiological indicators/Hormone	生理指标/激素 Physiological indicators/Hormone	相关性 Correlation
SS	IAA	0.902^*
SS	CKs	0.914^*
SS	GA	0.944^**
SS	JA	0.886^*
SS	SA	0.958^**
IAA	CKs	0.841^*
IAA	GA	0.899^*
IAA	JA	0.944^**
GA	CKs	0.936^**
GA	JA	0.915^*
GA	SA	0.896^*
CKs	SA	0.879^*

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