Abstract: Functional components in the ethanol extraction of Brevibacillus brevis FJAT-0809-GLX were determined by means of GC/MSD.Twenty four compounds were identified.Among them,12 displayed over 90% match with their respective standards.The relative contents of ethylparaben and 1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester were 24.28% and 19.78%,respectively.Their total contents were significantly higher than that of other substances other substances found in the analysis.These two compounds were speculated to be associated with the antibacterial activity of Brevibacillus brevis FJAT-0809-GLX.The ethanol extraction of B.brevis FJAT-0809-GLX had an inhibitory effect on Escherichia coli K88,among them,the inhibition zone diameter of ethylparaben was 17.81 mm.It was also found to inhibit the activity of peroxidase(POD).According to their functions,the identified substances were classified as either antibacterial or volatile flavor compounds.