羧化壳聚糖对蚊净香草外植体诱导过程酶活性的影响

Effect of N-carboxymethylchitosan on Enzyme Activity of Pelargonium spp.cv Citronella Callus

摘要: 以蚊净香草叶柄和叶片为外植体,研究不同浓度羧化壳聚糖对其诱导过程各种酶活性的影响。结果表明,在蚊净香草叶柄初代培养基中,附加4g·L-1羧化壳聚糖使过氧化物酶活性比对照组提高8.57倍;附加2g·L-1羧化壳聚糖使吲哚乙酸氧化酶活性比对照组提高了3倍;叶片初代培养基中,附加2g·L-1羧化壳聚糖中硝酸还原酶活性是对照组的3.5倍。说明羧化壳聚糖能提高蚊净香草叶柄和叶片中相关酶活性,从而使愈伤组织诱导的效率更高。

Abstract: The petioles and leaves of Pelargonium spp.cv Citronella were used to study the effect of N-carboxymethylchitosan(NCMC) in varying concentrations on the enzymatic activities of the explants.The results indicated that the activities of POD,NR and IAA-oxidase increased to different levels in the explants due to the presence of NCMC in the medium.Specifically,the POD activity increased 8.57 times as compared to the control with the application of 4 g·L-1 NCMC,and the activities of NR and IAA-oxidase increased 3.5 times and 3 times,respectively,of the control group with the application of 2 g·L-1 NCMC.It suggested that NCMC could encourage callus formation on the petioles and leaves of Pelargonium spp.cv Citronella.