Abstract: In this paper,a strain of agaricus blazei named J1 was used as original strain.Using 60CO-UV compound mutation,a mutant strain was got that had significant differences with J1 in morphology.The result of fruiting experiment showed that the agronomic characters of the mutant strain including color of fruiting body,the average weight per fruitbody,the diameter of pileus,the thickness of pileus,the diameter of stalk,the height of stalk,and so on,was significantly different with J1,and the yield increased significantly.Biological characteristic research indicated∶ the mycelium growth rate of mutant strain and J1 has significant differences in different temperature,pH and ratio of material to water.The mycelium of mutant strain and J1 were observed by means of Scanning Electron Microscopy,and the results showed that the mycelium of mutant strain with particulate on surface was thinner than J1.The analysis of SSR marks fingerprint showed that the mutant strain and J1 had obvious differences.Series of studies showed that the mutant strain which was named "FUJI J77" is a new strain that has great potential for development.