藏灵菇发酵乳中醋酸菌的分离纯化与鉴定

Isolation and Identification of Acetobacter in Tibetan Kefir

摘要: 从藏灵菇中分离出醋酸菌株FJAT-13797, 利用形态学观察、生理生化和16SrDNA方法对其进行细菌学鉴定, 菌体形态为圆端直杆, 单个或成对, 无芽孢, 其生理生化特征与醋杆菌属 (Acetobacter) 基本一致。经16SrDNA基因序列同源性比较和系统发育分析, 菌株FJAT-13797鉴定为东方醋酸菌 (Acetobacter orientalis) 。

Abstract: One Acetobacter strain, FJAT-13797, was isolated from Tibetan kefir.The image under scanning electronic microscope showed that the bacterium was rod-shaped with round ends, and presented in single or pairs without spores.The strain showed the bio-chemical properties of Acetobacter spp.The 16S rDNA sequence analysis indicated that the strain, FJAT-13797, belonged to Acetobacter orientalis.