Isolation and Molecular Identification of Ammonia-nitrogen Degrading Bacterium,JN-2
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摘要: 研究降解污水中氨氮污染物的微生物资源和分子系统特征。从大型养殖场的活性污泥中分离到1株能降解氨氮的菌株JN-2。利用筛选培养基分离得到氨氮降解菌,对菌株进行生理生化试验和16SrDNA基因测序,并构建系统发育树。结果表明JN-2菌株在28℃和pH 7条件下,24h内可以将初始质量浓度为200mg·L-1的NH4+-N降解75.6%。对构建的菌株系统发育树进行分析,发现菌株JN-2与苍白杆菌属的同源性达100%,因此推断JN-2菌属于苍白杆菌,菌株的16SrDNA序列在GenBank的登录号为JX535021。游离菌株对养殖污水的氨氮处理效率为30.1%,固定化小球对养殖污水的氨氮处理效率为62.8%。Abstract: This paper reports the resources and molecular phylogeny of the microbes that degrade ammonia-nitrogen pollutants in sewer.An efficient strain,JN-2,was isolated from the sludge in a large livestock farm.A screening medium was used to isolate the strain.Its biochemical characteristics and 16S rDNA gene sequence were determined to construct the phylogenetic tree.The results indicated that JN-2′s NH+4-N degrading efficiency per 24 h at 28℃ and pH 7 was 75.6% of its initial concentration of 200 mg·L-1.The accession number of its 16S rDNA gene sequence in GeneBank was JX535021.The phylogenetic tree showed that JN-2 had an 100% homology to Ochrobactrum sp..The NH+4-N reduction on sewer by the free-state JN-2 was 30.1%,and by the immobilized strain,62.8%.
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