Abstract: The aim of this study was fractionate and analysis of feather peptide for feed.5 components contented in feather peptide meal were separated by AKTA purifier 100 with Sephacryl S-100.The molecular weight of them were as followed: >2 000 000,6 456.54,758.58,398.11,186.21.Resultsof fractionation showed the fifth component were Asp,Thr,Ser,Glu.The fourth component were dipeptide or tripeptide,which content Asp,Thr,Ser,Gly,Ala.Amino acid composition of the third component,six peptide or seven peptide,were as followed: Asp,Thr,Ser,Glu,Gly,Ala.Peptide length of component 2,whose amino acid composition is the same as component 1,was close to antibacterial peptide.All these showed feather peptide meal was one kind of potential protein source for feed as it was rich in small peptides.