水稻OsAPX基因的克隆及过表达载体的构建

Cloning and Construction of Over-expression Vector of OsAPX Gene

摘要: 通过RT-PCR法从粳稻日本晴中克隆到ascorbate peroxidase(APX)基因,该基因的cDNA长为768bp,包含完整的CDS序列。将克隆到的片段连接到植物表达载体pBI121的相应位置,构建1个APX基因的过表达载体pBI121-APX。利用农杆菌EHA105介导该植物表达载体在籼稻“多系1号”和“航1号”中的遗传转化,以期成功获得转基因植株,为后期研究APX基因在水稻耐储藏方面所发挥的功能打下基础。

Abstract: A full length cDNA of ascorbate peroxidase(APX) gene was cloned from Oryza sativa L.Japonica Nipponbare with RT-PCR.It was 768 bp including the CDS,and was connected to the plant expression binary vector,pBI121,to construct an over-expression vector of APX gene.The pBI121-APX vector was transformed into indica rice,Duoxi No.1 and Hang No.1,mediating by Agrobacterium tumefaciens EHA105.With this,transgenic plants could be obtained for further study on the function of APX gene in rice storability.