Abstract: The metabolites of Ralstonia solanacearum with various pathogenic were analysed by metabonomic method based on LC/Q-TOF MS.Significant metabolic markers were used for classification pathogenic of Ralstonia solanacearum by PCA analysis,and PLS-DA prediction model were established.The result suggest that metabonomic analysis could be used for indentify virulent Ralstonia solanacearum strain and avirulent strain and Tn-5mutant strains.Furthermore,there were 12metabolites showed similar trend and had significant differences between virulent strain and avirulent strains(both wide-type and Tn-5mutant),suggesting that the 12metabolites are probably related to the pathogincity of Ralstonia solanacearum.