Abstract: Based on morphological,physiological and biochemical characteristics,combined with analysis of 16S rDNA sequences,the strain TL2was identified as Bacillus subtilis.The inhibitive efficacy of Bacillus subtilis TL2 to ten kinds of fungal pathogens were tested by dual culture.The result showed that Bacillus subtilis TL2 has significant inhibition to ten pathogenic fungi after 5dwith dual culture.The best average inhibitive rate of Bacillus subtilis TL2to Phytophthora capsici was 65.12%and the lowest average inhibitive rate of Bacillus subtilis TL2to Botryosphaeria dothidea was 24.14%.