Abstract: The gross morphologic and histopathologie changes of 110 day-old female sheldrake ducks were analyzed after experimentally infected with Tembusu virus. The most prominent gross changes were hyperemia, hemorrhage and degeneration in ovarian follicle. Histopathologic changes were hyperemia, hemorrhage of ovarian follicle, necrosis of granulosa cells, and infiltration of heterophils in ovary and lysis of egg yolk. Other lesions were proliferation of connective tissue and bile duct in interstitium of live, infiltration of lymphocyte in the live, lung, kidney, pancreas and glandular stomach. The results showed that infection of Tembusu virus in sheldrake ducks is correlated highly with ovarian lesions, which showing hyperemia, hemorrhage and degeneration in ovarian follicle, and infiltration of heterophils in the ovary. This study of gross morphologic and histopathologic changes of infected ducks was helpful for clinical rapid diagnosis of the duck Tembusu virus disease.