Abstract: Selected biological characteristics of Pleurotus centralis Yu,Chen Yao,sp.nov.ined.2012,collected from the natural forest located in Putian,Fujian Province.The results showed that the range of temperature for the growth of P.centralis was 10~35℃,and the optimum was 25℃;the range of temperature for mycelial growth was 10~35℃,the optimum was 25℃;the lethal temperature for the spores and the mycelium was 45℃ 25min and 50℃15min.The mycelial growth of P.centralis could go under the range of pH 4~10,the optimum being 6.Sucrose,lactose,soluble starch,glucose,fructose and maltose were suitable for the mycelia growth and maltose was the best.Beef anointed,peptone,yeast extract,NH4NO3,KNO3and NH4Cl could be used as nitrogen sources and peptone was the best.For the mycelial growth of P.centralis,the range of C/N was 15:1~60:1,and the C/N of 25:1~35:1was the most suitable.The fruit body formation need illumination;the difference is not remarkable between the continuous illumination and alternation of dark(12h)and illumination(12h).But the fruit body could not form under absolute darkness.