草鱼致病性类志贺邻单胞菌的分子鉴定及快速检测

Molecular Identification and Rapid Detection of Pathogenic Plesiomonas shigelloides from Ctenopharyngodon idellus

摘要: 应用原核生物16SrRNA基因通用引物对草鱼致病菌株LCCiL90625NA进行16SrRNA基因的克隆、序列分析。核酸序列同源性分析表明, 该序列与Plesiomonas shigelloides菌株PIC3的16SrRNA基因序列 (NCBI登录号:GQ359957) 同源性最高, 为99.7%。同时, 通过与弧菌科代表菌株16SrRNA基因构建的发育进化树表明, 该菌株与已登录的类志贺邻单胞菌聚为一类。设计引物扩增Plesiomonas shigelloides 23SrRNA基因的特异性片段进一步证实分离菌株为类志贺邻单胞菌, 同时证明该引物可以用于类志贺邻单胞菌的快速检测。

Abstract: A Gram negative pathogenic bacterium named strain LCCiL90625NA was isolated from the liver samples of diseased Ctenopharyngodon idellus and was indentified by phylogenetic analysis of 16SrRNA.A phylogenetic tree was constructed based on 16SrDNA sequences of strain LcCiL90625NA and other related bacteria species in the GenBank.It revealed that strain LcCiL90625NA shared a high similarity of 99.7% with Plesiomonasshigelloides and formed a cluster with strain PIC3 (accession number GQ359957) .The isolated bacterium strain LcCiL90625NA was recognized as Plesiomus shigelloides.PCR primers were designed to amplity a 280-bp flagment from the Plesiomusshigelloides23SrRNA gene.The results indicated that this method might serve as an efficient tool for rapid and sensitive identification of Plesiomus shigelloides.