Abstract: A pair of primers were designed and synthesized according to the nanostructure protein (NS1) gene of avian Tenbusu virus WR strain available in GenBank.The NS1gene was amplified by RT-PCR and cloned into the prokaryotic expression vector pGEX-4T-1, then induced into E.coli with IPTG.The SDS-PAGE analysis of expressed protein demonstrated that the recombinant NS1protein of 62kD had got a high expression, which laid a good foundation for developing in molecular diagnosis and the function of the NS1gene for avian Tenbusu virus.