Abstract: The effects of NH+ 4 and NO3 levels and BA, NAA on vitro proliferation and rooting of Ophiopogon japonicus (L.F) Kew-Gawl were compared with vitro culture, Ophiopogon japonicus (L.F) Kew-Gawl to study the conditions of Ophiopogon japonicus (L.F) Kew-Gawl.The results showed that the coefficient of japonicus proliferation was 2.60under the optimum ratio of NH+ 4 to NO3 was 2.2∶1with BA.Further, the proliferation with BA was higher than that without BA at the same condition.In addition, the root proportions of different nitrogen conditions were also different.When the ratio of NH+ 4 to NO3 was 1.7∶1, the situation was the best for rooting without NAA.The trend of rooting was almost the same between with NAA and without NAA.But it was not rooting when the ratio of NH+ 4 to NO3 was 2∶1.