Abstract: The biological characteristics of plant vaccin strain (nonpathogenic Fusarium oxysporum strain FJAT9290) were investigated by compare the effects of incubation time, and nutritional conditions on its growth with a pathogenic strain as a control.The results showed that there was no significant difference of the mycelium dry weight between strain FJAT-9290with the pathogenic strain FJAT-282, the sporulation of strain FJAT-9290was 1.47×106 cfu·mL-1 on the 6th day, while the strain FJAT-282is 108.33×106 cfu·mL-1, and changes of pH values had no significant effect on the culture growth.Furthermore, the concentration of reducing sugar in culture filtrate of strain FJAT-9290decreased faster than strain FJAT-282;starch was conducive to the growth of mycelium of strain FJAT-9290, glucose was conducive to its sporulation, xylose was not conducive to the growth of mycelium of strain FJAT-282, fructose was conducive to its sporulation;organic nitrogen was benefit to the growth of mycelium, ammonium salt was not conducive to the growth of hypha, but also to the sporulation.In addition, nitrogen sources exercised a greater influence on colony morphology.