外源Ca2+对枇杷苗叶细胞钙分布及抗氧化系统的影响
Effects of Exogenous Ca2+ on the Intracellular Calcium Distribution and Antioxidant Enzymes Activities of Eriobotryajaponica Seedlings and Leaves
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摘要: 为探索一定浓度外源Ca2+在缓解枇杷冻害中所起到的积极作用, 以盆栽枇杷苗为试材, 先采用不同浓度CaCl2溶液喷施叶片, 并用焦锑酸钙沉淀法检验处理效果, 后采用人工降温的方法进行低温处理, 并对-3℃1.5h处理条件下的枇杷叶组织抗寒生理的相关指标进行测定。结果表明:0.0550mmol·L-1浓度的CaCl2溶液, 均能不同程度地降低枇杷苗MDA含量和提高SOD、POD、CAT及APX活性。其中, 以10mmol·L-1CaCl2溶液处理的SOD活性最高, 高于对照26.70%, 且达到显著水平;以25mmol·L-1 CaCl2溶液处理的APX活性最高, 高于对照6.39%, 但未达到显著水平。结论:在低温来临之前果园喷水防寒时, 喷施适宜浓度的CaCl2溶液有利于提高枇杷苗的抗寒性, 从而可以在一定程度缓解低温伤害。Abstract: CaCl2solution of different concentrattion were sprayed on the leaves of potted Eriobotryajaponica seedlings, the effects were verified by the calcium pyroantimonate precipitation method.Then, the seedlings were artificially treated at-3℃for 1.5h, and the cold resistance-relating physiological indices of the leaf tissues were measured.The results showed that CaCl2solutions of 0.05-50mmol·L-1 could all managed to decrease the MDA contents and to increase the SOD, POD, CAT and APX activities.The SOD activity of the control group was significantly elevated by 26.70% by using 10mmol·L-1 CaCl2solution.Although the APX activity of the control was maximally increased by 6.392% via 25 mmol·L-1 CaCl2solution, the upgrowth was still not significant.Thus, spraying appropriate CaCl2solution besides water in orchards prior to cold-air outbreaks was conducive to augmenting the cold-resistance indices of Eriobotryajaponica seedlings, thereby minimizing the low-temperature damages.