Abstract: The DNA fingerprints of xincui bitter gourd and its parents were analyzed by RAPD to obtain the genes differentially expressed.1out of the 120screened primers could amplify the characteristic polymorphic bands of F1generation with its female parent after repeated experiments.With the only one primer possessing high stability and repeatability, 6000individuals of Xincui bitter gourd from Hexi Corridor in Gansu province were identified, and our results ensured the promotion to Xincui bitter gourd, effectively.