Abstract: Aeromonas hydrophila MOMP-ISCOMs vaccine was prepared, and the vaccine was given to European eel by oral or injection.The European eel skin and kidney cells were separated and isolated after immunization 14 and 28days respectively, in which the antibody secreting cells (ASC) , were analyzed by ELISPOT technique.The results showed that the two immune ways could affect the number of immune cells in skin and kidneys in different amount of non-specific immune cells increased rapidly in oral immunization group, and specific immune cells increased more obviously in injection group.