Abstract: Peduncle axillary buds of the Little Cherry, Oncidium, were induced as explants.The orthogonal design was used to evaluate the effects of the culture medium containing inorganic substances, 6-BA, NAA and LH on the propagation of Oncidiumbuds.The result showed that 6-BA, the base medium, NAA and LH affected significantly on the bud multiplication.The optimal medium was the Improved Formulation No.1, which consisted of the base medium, 6-BA 3.0mg·L-1, NAA 0.1mg·L-1 and LH0.5g·L-1, and the average propagation coefficient was 6.53 in 45days.The breeding efficiency was effectively improved.The result appeared to provide a basis for the commercialization of the seedling propagation.